Ultra-slow oscillations in cortical networks in vitro

Mok, Siew-Ying; Nádasdy, Zoltán; Lim, Yang Mooi; Goh, Sing-Yau

doi:10.1016/j.neuroscience.2012.01.009

Cited by 18 publications

(31 citation statements)

References 30 publications

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“…Although the presence of synchronised array-wide activity has been reported previously in hiPSCderived neuronal cultures 10,27 , this reported behaviour did not progress further than the short SB firing that we observed at 40 DPP and did not evolve to the bi-stable period of oscillatory firing we report here. Indeed, this extended behaviour actually compares favourably to what has previously been described by several groups using similar MEA systems to study networks in dissociated rodent primary neurons 3,5,28,29 . In particular, the extended MAP/LAP oscillations we observed in hiPSC-derived neurons at 50DPP matches to those reported in rodent studies at ~20DPP 7,29 .…”

Section: Discussionsupporting

confidence: 77%

L-type voltage-gated calcium channel regulation of in vitro human cortical neuronal networks

Plumbly

Brandon

Deeb

et al. 2018

Preprint

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The combination of in vitro multi-electrode arrays (MEAs) and the neuronal differentiation of stem cells offers the capability to study human neuronal networks from patient or engineered human cell lines.Here, we use MEA-based assays to probe synaptic function and network interactions of hiPSC-derived neurons. Neuronal network behaviour first emerges at approximately 30 days of culture and is driven by glutamate neurotransmission. Over a further 30 days, inhibitory GABergic signalling shapes network behaviour into a synchronous regular pattern of burst firing activity and low activity periods. Gene mutations in L-type voltage gated calcium channel subunit genes are strongly implicated as genetic risk factors for the development of schizophrenia and bipolar disorder. We find that, although basal neuronal firing rate is unaffected, there is a dose-dependent effect of L-type voltage gated calcium channel inhibitors on synchronous firing patterns of our hiPSC-derived neural networks. This demonstrates that MEA assays have sufficient sensitivity to detect changes in patterns of neuronal interaction that may arise from hypo-function of psychiatric risk genes. Our study highlights the utility of in vitro MEA based platforms for the study of hiPSC neural network activity and their potential use in novel compound screening.

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Section: Discussionsupporting

confidence: 77%

L-type voltage-gated calcium channel regulation of in vitro human cortical neuronal networks

Plumbly

Brandon

Deeb

et al. 2018

Preprint

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“…Similar burst motifs were observed in experiment conducted by Mok et al [5] and Volman et al [17]. Based on the activity pattern of the single-unit activity and the burst motifs [5], we postulate that there are two or more distinct sub-networks in the cortical culture.…”

Section: Sub-networksupporting

confidence: 86%

“…Ultra-slow oscillations with frequencies less than 0.01Hz were reported in other experiments [5][6][7][8]. A recent study by Mok et al [5] reported on ultra-slow oscillations in MEA cultures of rat cortical neurons. The ultra-slow oscillations were characterized by large synchronized bursts at the peaks and smaller bursts at the troughs.…”

Section: Introductionmentioning

confidence: 69%

“…Slow oscillations with frequency less than 1Hz were detected in various in vivo experiments [2][3][4]. Ultra-slow oscillations with frequencies less than 0.01Hz were reported in other experiments [5][6][7][8]. A recent study by Mok et al [5] reported on ultra-slow oscillations in MEA cultures of rat cortical neurons.…”

Section: Introductionmentioning

confidence: 90%

“…In this study, numerical simulations of a network of IF neurons were conducted in an attempt to simulateultra-slow oscillations observed by Mok et al [5]. All equations in this simulation were solved using the Runge-Kutta 4th order method [18].…”

Section: Simulationmentioning

confidence: 99%

See 2 more Smart Citations

Simulation of Ultra-slow Oscillations Using the Integrate and Fire Neuron Model

Ng¹,

Ying²,

Cheng³

et al. 2012

ENG

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The Integrate and Fire (IF) neuron model wasusedto simulate ultra-slow oscillations that were observed in cortical cultures. Simulation of a network with 2 sub-networks is conducted in this study. We introduced an additional equation that governs the generation and dissipation of an inhibitory property to each of the sub-network.Sub-networks that fire at different rate are generated from the simulation. The network activity from the simulation oscillates at frequencies that are comparable to ultra-slow oscillations observed in cortical cultures.

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A simple model of cortical culture growth: burst property dependence on network composition and activity

Kawasaki

Stiber

2014

Biol Cybern

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This paper describes large-scale simulations of growth, network formation, and behavior in cultures of dissociated cortical cells. A neuron model that incorporates synaptic facilitation/depression and neurite outgrowth/retraction was used to construct virtual cultures of 10,000 cells whose spiking behavior and evolution were investigated in closed-loop simulations. This approach allows us to perform detailed analysis of the effects of model parameters on burst shape and timing, their changes, and the interrelationship among these behaviors, gross network structure, and model parameters. We examined the effects of two parameters--network composition (fraction of excitatory cells) and neuron excitability (activity level corresponding to neurite outgrowth equilibrium)--on network structure and behavior. Our results suggest that much of the burst shape and timing observed in vitro can be explained by a model that includes only closed-loop neurite outgrowth and dynamic synapses; features such as LTP/LTD, random connectivity, long-distance connections, and detailed neurite topology are not necessary.

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Ultra-slow oscillations in cortical networks in vitro

Cited by 18 publications

References 30 publications

L-type voltage-gated calcium channel regulation of in vitro human cortical neuronal networks

L-type voltage-gated calcium channel regulation of in vitro human cortical neuronal networks

Simulation of Ultra-slow Oscillations Using the Integrate and Fire Neuron Model

A simple model of cortical culture growth: burst property dependence on network composition and activity

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