Ultra-Stable oligonucleotide-gold and -silver nanoparticle conjugates prepared by a facile silica reinforcement method

Poly(ethylene glycol)- and Carboxylate-Functionalized Gold Nanoparticles Using Polymer Linkages: Single-Step Synthesis, High Stability, and Plasmonic Detection of Proteins

“…PEG-OCH 3 -terminated gold nanoparticles were synthesized using the Turkevich–Frens method and the ligand exchange reaction with HS-PEG-OCH 3 . − A HAuCl 4 aqueous solution (50 mL, 0.25 mM) was refluxed. A sodium citrate aqueous solution (0.50 mL, 0.034 M) was added to the solution and vigorously mixed for 2 min.…”

“…Stability Test. PEG-OCH 3 -terminated gold nanoparticles were synthesized using the Turkevich−Frens method 40 and the ligand exchange reaction with HS-PEG-OCH 3 . 26−28 A HAuCl 4 aqueous solution (50 mL, 0.25 mM) was refluxed.…”

“…These molecules are involved in many enzymatic reaction buffers, , and thus, Au nanoparticles functionalized via a Au–S bond undergo rapid and irreversible aggregation. Mattoussi et al successfully used multiple thiol ligands, such as di- and tetrathiols, to greatly enhance the colloidal stability under extreme conditions. , Recently, Lee et al reported a synthetic method of enhancing the chemical stability of monothiol-modified oligonucleotide–Au nanoparticle conjugates using a thin silica coating …”

“…38,39 Recently, Lee et al reported a synthetic method of enhancing the chemical stability of monothiol-modified oligonucleotide−Au nanoparticle conjugates using a thin silica coating. 40 Although these approaches have been successfully employed in bioapplications, there is further demand for highly stable colloids with various functionalities on their surface that can work with any biological media. Polymer surfactants are known to be effective for the confinement of the nanoparticle surface and for control of the particle morphology.…”