Ultra-structure characterization of self-etching treated cementum surfaces

Abstract

Objectives: to evaluate the effect of different conditioning treatments on surface roughness and topography of dental cementum. Study Design: Extracted human canines were used for the present study. The mesial surface from the cervical third of the roots were ground flat with wet 600-grit silicon carbide paper. They were polished (up to 1/4 µm diamond paste) and treated as follows: 1) No treatment, 2) 35% H3PO4 during 15 s, 3) Clearfil SE Bond primer (SEB), 4) One-Up Bond F (OUB). The adhesive systems were applied following manufacturer's instructions. SEB primer and OUB were removed from surfaces by washing and ultrasonic agitation with ascending ethanol solutions. Digital images of treated surfaces (5x5 and 15x15 µm) were obtained by means of an atomic force microscope (AFM) analysis. The average surface roughness (Ra nanometers) of the scanned areas was assessed. Data were analyzed by ANOVA and SNK multiple comparisons tests (p<0.05). Results: phosphoric acid treatment produced the highest mean roughness value, at all scan sizes. At 5x5 µm AFM images, for self-etch adhesive systems no differences in roughness were detected. At 15x15 µm, when One-Up Bond F was employed the lowest value was obtained. Conclusions: When phosphoric acid treatment was applied, cementum surface roughness increased and a strong demineralization with exposed collagen fibers could be observed.

In vitro biocompatibility of a dentine substitute cement on human MG63 osteoblasts cells: Biodentine™ versus MTA®

“…The results of the AFM analysis revealed that the difference in chemical composition between Biodentine TM and MTA â , such as the absence of calcium aluminate and other components in Biodentine TM (Table 1), did not modify surface roughness and topography of dental cements. Aguilera et al (2011) demonstrated that surface treatments with H 3 PO 4 did not change surface roughness of dental cements. Surface analyses of Biodentine TM and MTA â using AFM revealed an intricate pattern of submicron-sized concavities and nanoscale surface roughness.…”

“…Aguilera et al . () demonstrated that surface treatments with H 3 PO 4 did not change surface roughness of dental cements. Surface analyses of Biodentine™ and MTA ® using AFM revealed an intricate pattern of submicron‐sized concavities and nanoscale surface roughness.…”

Stem cells of the lamina propria of human oral mucosa and gingiva develop into mineralized tissues in vivo

“…, Aguilera et al. ). These observations indicate the biological nature of the mineralization process of these matrices.…”

Effect of aluminum chloride hemostatic agent on microleakage of class V composite resin restorations bonded with all-in-one adhesive

“…It has been demonstrated that if surface contaminants are not removed a proper bond will not be achieved and debonding will occur. In such a case, bacteria, ions and molecules penetrate through the gaps between the tooth structure and restorative materials, resulting in microleakage due to capillarity (7). …”

“…Recently new all-in-one adhesive systems (7th generation one-step self-etch) have been introduced, which have simplified the bonding process. These systems do not require separate etching, rinsing and drying steps; as a result, the possibility of dentin desiccation is eliminated and the time needed for a restorative procedure decreases (6,7). In addition, these systems are not as technique-sensitive as total-etch adhesives, which is considered an advantage.…”