Ultrabright and Multicolorful Fluorescence of Amphiphilic Polyethyleneimine Polymer Dots for Efficiently Combined Imaging and Therapy

Sun, Yun; Cao, Weipeng; Li, Shengliang; Jin, Shubin; Hu, Kelei; Hu, Liming; Huang, Yuanyu; Gao, Xueyun; Wu, Yan; Liang, Xing‐Jie

doi:10.1038/srep03036

Cited by 81 publications

(79 citation statements)

References 32 publications

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“…Details of the synthesis and characterizations have been reported in the experimental section. We have developed blue fluorescent polymer dots (PDs) using amphiphilic polymer solvent evaporation technique 17 . Synthesis details and characterizations have been reported in the experimental section.…”

Section: Resultsmentioning

confidence: 99%

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Designing a multicolor long range nanoscopic ruler for the imaging of heterogeneous tumor cells

et al. 2016

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Tumor heterogeneity is one of the biggest challenges in cancer treatment and diagnosis. Multicolor optical ruler is essential to address heterogeneous tumor cells complexity. Driven by the need, current article reports the design of multicolor long range nanoscopic ruler for screening tumor heterogeneity by accurately identifying epithelial cells and cancer stem cells (CSCs) simultaneously. Nanoscopic surface energy transfer (NSET) ruler has been developed using blue fluorescence polymer dots (PDs) and red fluorescence gold cluster dots (GCDs) as multicolor fluorescence donor and plasmonic gold nanoparticle (GNP) acts as an excellent acceptor. Reported experimental results demonstrated that the multicolor nanoscopic ruler’s working window is above 35 nm distances, which is more than three times farther than that of Förster resonance energy transfer (FRET) distance limit. Theoretical modeling using Förster dipole–dipole coupling and dipole to nanoparticle surface energy transfer have been used to discuss the possible mechanism for multicolor nanoscopic ruler’s long-range capability. Using RNA aptamer that are specific for the target cancer cells, experimental data demonstrate that nanoscopic ruler can be used for screening epithelial and CSCs simultaneously from whole blood sample with 10 Cells/mL detection capability. Experimental data show that nanoscopic ruler can distinguish targeted cells from non-targeted cells.

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Section: Resultsmentioning

confidence: 99%

“…Last few years, due to very good photostability and bio-compatibility, polymer dots (PDs) and gold cluster dots (GCDs) have emerged as bright fluorescence imaging probes 17–29 . Since the nanoparticles have huge surface area, it allows chemical conjugation of multiple acceptors like polymer dots and gold dots together onto its surface.…”

Section: Introductionmentioning

confidence: 99%

Designing a multicolor long range nanoscopic ruler for the imaging of heterogeneous tumor cells

et al. 2016

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“…93 pH-dependent variation of the PD structure rigidity was found to influence the fluorescence signal. Antitumor efficacy was assessed after intravenous injection of the PDs in MCF-7 tumor-bearing mice (compared to free Ptx) and ex vivo fluorescence confirmed the tumor accumulation.…”

Section: Miscellaneousmentioning

confidence: 94%

Optical Imaging and Chemotherapy

Bort

Mura

Couvreur

2016

Nanotheranostics for Personalized Medicine

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Nanoscale drug delivery systems which combine in a single tool imag ing and therapeutic functions, (i.e., nanotheranostics) have attracted a great deal of interest due to the possibility to monitor in real-time both distribution/drug release and the effectiveness of the treatment thus, allowing an immediate adjustment of the therapeutic protocol. Among the various modalities, this chapter will focus on the optical imaging combined to the delivery of anticancer drugs and the main achievement obtained in this fi eld will be discussed. Nanotheranostics displaying multi-imaging properties will be also presented.Nanotheranostics for Personalized Medicine Downloaded from www.worldscientific.com by NANYANG TECHNOLOGICAL UNIVERSITY on 06/21/16. For personal use only.

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“…38 The fluorescence characteristics of FBNPs were further analyzed by fluorescence spectrophotometry. As shown in Fig.…”

Section: Fluorescence Characterization Of Cross-linked Npsmentioning

confidence: 99%

pH-responsive biocompatible fluorescent polymer nanoparticles based on phenylboronic acid for intracellular imaging and drug delivery

Cao

et al. 2014

Nanoscale

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To address current medical challenges, there is an urgent need to develop drug delivery systems with multiple functions, such as simultaneous stimuli-responsive drug release and real-time imaging. Biocompatible polymers have great potential for constructing smart multifunctional drug-delivery systems through grafting with other functional ligands. More importantly, novel biocompatible polymers with intrinsic fluorescence emission can work as theranostic nanomedicines for real-time imaging and drug delivery. Herein, we developed a highly fluorescent nanoparticle based on a phenylboronic acid-modified poly(lactic acid)-poly(ethyleneimine)(PLA-PEI) copolymer loaded with doxorubicin (Dox) for intracellular imaging and pH-responsive drug delivery. The nanoparticles exhibited superior fluorescence properties, such as fluorescence stability, no blinking and excitation-dependent fluorescence behavior. The Dox-loaded fluorescent nanoparticles showed pH-responsive drug release and were more effective in suppressing the proliferation of MCF-7 cells. In addition, the biocompatible fluorescent nanoparticles could be used as a tool for intracellular imaging and drug delivery, and the process of endosomal escape was traced by real-time imaging. These pH-responsive and biocompatible fluorescent polymer nanoparticles, based on phenylboronic acid, are promising tools for intracellular imaging and drug delivery.

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Ultrabright and Multicolorful Fluorescence of Amphiphilic Polyethyleneimine Polymer Dots for Efficiently Combined Imaging and Therapy

Cited by 81 publications

References 32 publications

Designing a multicolor long range nanoscopic ruler for the imaging of heterogeneous tumor cells

Designing a multicolor long range nanoscopic ruler for the imaging of heterogeneous tumor cells

Optical Imaging and Chemotherapy

pH-responsive biocompatible fluorescent polymer nanoparticles based on phenylboronic acid for intracellular imaging and drug delivery

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