Ultrabright NIR-II Nanoprobe for Image-Guided Accurate Resection of Tiny Metastatic Lesions

Xu, Huihui; Yuan, Lishan; Shi, Qiankun; Tian, Ye; Hu, Fang

doi:10.1021/acs.nanolett.3c04483

Cited by 12 publications

(1 citation statement)

References 33 publications

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“…A site-specific cascade-activated strategy that offers tunable properties (such as size- and charge-switchable behavior, activatable fluorescence) in response to tumor environmental stimuli would enable specific and accurate diagnosis of hidden metastases foci. − Notably, the presence of primary site efficiently obstructs the activation of secondary site, thereby preventing premature activation by low-level indicators present in normal tissues before accumulation of imaging agents in tumors. − Therefore, the site-specific cascade-activatable fluorogenic probes allow for a fluorescence response to orthogonal indicators in situ , facilitating highly specific and sensitive diagnostic imaging of elusive lung metastases.…”

Section: Introductionmentioning

confidence: 99%

Site-Specific Cascade-Activatable Fluorogenic Nanomicelles Enable Precision and Accuracy Imaging of Pulmonary Metastatic Tumor

Chen,

Liu,

Zhang

et al. 2024

JACS Au

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The precise localization of metastatic tumors with subtle growth is crucial for timely intervention and improvement of tumor prognosis but remains a paramount challenging. To date, site-specific activation of fluorogenic probes for single-stimulusbased diagnosis typically targets an occult molecular event in a complex biosystem with limited specificity. Herein, we propose a highly specific site-specific cascade-activated strategy to enhance detection accuracy, aiming to achieve the accurate detection of breast cancer (BC) lung metastasis in a cascade manner. Specifically, cascade-activatable NIR fluorogenic nanomicelles HPNs were constructed using ultra-pH-sensitive (UPS) block copolymers as carriers and nitroreductase (NTR)-activated fluorogenic reporters. HPNs exhibit programmable cascade response characteristics by first instantaneous dissociating under in situ tumor acidity, facilitating deep tumor penetration followed by selective fluorescence activation through NTR-mediated enzymatic reaction resulting in high fluorescence ON/OFF contrast. Notably, this unique feature of HPNs enables high-precision diagnosis of orthotopic BC as well as its lung metastases with a remarkable signal-tobackground ratio (SBR). This proposed site-specific cascade activation strategy will offer opportunities for a specific diagnosis with high signal fidelity of various insidious metastatic lesions in situ across different diseases.

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Section: Introductionmentioning

confidence: 99%

Site-Specific Cascade-Activatable Fluorogenic Nanomicelles Enable Precision and Accuracy Imaging of Pulmonary Metastatic Tumor

Chen,

Liu,

Zhang

et al. 2024

JACS Au

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Organic NIR‐II Nanofluorophore with Ultrahigh Quantum Yield for Vessels Imaging and Fluorescence Image‐Guided Surgery

Zhang,

Li,

Ren

et al. 2024

Adv Funct Materials

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The second near‐infrared window (NIR‐II) fluorescence imaging has aroused more and more interest in biomedical applications due to negligible autofluorescence and deep penetration ability. However, the fluorescence quantum yield of NIR‐II fluorophores with more than 1100 nm emission wavelength is typically less than 1%, thus limiting the imaging quality of deep tissues. In this study, a novel NIR‐II organic small molecule CTB1125 is designed and synthesized. Due to an efficient intramolecular charge transfer (ICT) facilitated by a high donor‐acceptor‐donor (D‐A‐D) strength structure, CTB1125 exhibits a maximum emission wavelength of 1125 nm. Additionally, branched alkyl chains are modified on the conjugated fused ring backbone to reduce intermolecular stacking. CTB1125 is encapsulated in the amphiphilic copolymer PS2000‐PEG2000 to form CTB1125‐NPs, which exhibit ultrahigh fluorescence quantum yield up to 4.84% in aqueous solution, excellent photostability, and high signal‐to‐noise rate. Due to these outstanding properties, CTB1125‐NPs have been successfully used for clear and long‐duration NIR‐II vessel imaging with small amounts of CTB1125‐NPs, low laser power and short exposure time, and precisely guiding the surgical resection of tumors in mice through fluorescence imaging. This work provides a promising NIR‐II fluorophore with ultrahigh brightness for deep‐tissue imaging and imaging‐guided surgery.

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Rational Design of NIR‐II Emitting Conjugated Polymer Derived Nanoparticles for Image‐Guided Cancer Interventions

Gill,

Srivastava,

Tropp

2024

Adv Healthcare Materials

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Due to the reduced absorption, light scattering, and tissue autofluorescence in the NIR‐II (1000–1700 nm) region, significant efforts are underway to explore diverse material platforms for in vivo fluorescence imaging, particularly for cancer diagnostics and image‐guided interventions. Of the reported imaging agents, nanoparticles derived from conjugated polymers (CPNs) offer unique advantages to alternative materials including biocompatibility, remarkable absorption cross‐sections, exceptional photostability, and tunable emission behavior independent of cell labeling functionalities. Herein we summarize the current state of NIR‐II emitting CPNs and highlight structure‐function‐property relationships that can be used to elevate the performance of next‐generation CPNs. Methods for particle processing and incorporating cancer targeting modalities are discussed, as well as detailed characterization methods to improve interlaboratory comparisons of novel materials. We then highlight contemporary methods to specifically apply CPNs for cancer diagnostics and therapies. This review not only summarizes the current state of the field, but offers future directions and provides clarity to the advantages of CPNs over other classes of imaging agents.This article is protected by copyright. All rights reserved

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Ultrabright NIR-II Nanoprobe for Image-Guided Accurate Resection of Tiny Metastatic Lesions

Cited by 12 publications

References 33 publications

Site-Specific Cascade-Activatable Fluorogenic Nanomicelles Enable Precision and Accuracy Imaging of Pulmonary Metastatic Tumor

Site-Specific Cascade-Activatable Fluorogenic Nanomicelles Enable Precision and Accuracy Imaging of Pulmonary Metastatic Tumor

Organic NIR‐II Nanofluorophore with Ultrahigh Quantum Yield for Vessels Imaging and Fluorescence Image‐Guided Surgery

Rational Design of NIR‐II Emitting Conjugated Polymer Derived Nanoparticles for Image‐Guided Cancer Interventions

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