Ultracytochemical localization of adenylate cyclase in early human placenta

Spreca, A; Rambotti, Maria Grazia; Ferrarese, R; Pomili, Giorgio; Angelini, Antonio

doi:10.1002/ar.1092200407

Cited by 7 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar to previous observations on ultracytochemical localization of particulate GC activity (Rambotti et aI., 1987(Rambotti et aI., , 1988Spreca et al, 1989;Rambotti et al, 1990;Spreca et al,, 1991), here we provide morphological evidence that GC reaction product was detected on plasma membranes even if there is some localization of GC reaction product on intracellular membranes. Recently we detected the presence of rANF and pBNP GC capable of stimulating on intracellular membranes in rat adrenal gland (Rambotti et aL, 1993) and in rat neurohypophysis (Rambotti et al, 1994).…”

Section: Discussionsupporting

confidence: 78%

Ultracytochemical localization of particulate guanylate cyclase after stimulation with natriuretic peptides in lamb olfactory mucosa

Spreca

Rambotti

1994

Histochem J

Self Cite

View full text Add to dashboard Cite

The ultracytochemical localization of particulate guanylate cyclase has been studied in lamb olfactory mucosa after activation with rat atrial natriuretic factor (rANF), porcine brain natriuretic peptide (pBNP), porcine C-type natriuretic peptide (pCNP) or rat brain natriuretic peptide (rBNP). Particulate guanylate cyclase is the receptor for these peptides and recently two subtypes of the cyclase have been identified. These isoforms are stimulated differently by ANF, BNP and CNP. Under our experimental conditions, rANF, pCNP and pBNP were strong activators of particulate guanylate cyclase in lamb olfactory mucosa, as demonstrated by the presence of reaction product. Samples incubated in basal conditions without rANF, pCNP or pBNP, or samples incubated in presence of rBNP did not reveal any cyclase activity. The rANF-stimulated cyclase activity was localized in the apical portion of olfactory epithelium. pCNP-stimulated guanylate cyclase was detected to the lamina propria in association with secretory cells of Bowman's glands and with cells in close relation with Bowman's glands (elongated cells and myoepithelial cells). The cyclase activity stimulated by pBNP was limited to cells of Bowman's glands. The present data indicate that ANF and CNP are recognized by different receptors and that BNP and CNP bind to the same receptor.

show abstract

Section: Discussionsupporting

confidence: 78%

Ultracytochemical localization of particulate guanylate cyclase after stimulation with natriuretic peptides in lamb olfactory mucosa

Spreca

Rambotti

1994

Histochem J

Self Cite

View full text Add to dashboard Cite

show abstract

“…cAMP [38] and membrane-bound stimula tory and inhibitory guanine nucleotide regula tory proteins, Gs and Gi [39], are localized in cytotrophoblasts and syncytiotrophoblasts in early human placenta. Cholera toxin and forskolin stimulated adenylate cyclase and cAMP and hCG production [39,40], suggesting cou pling of Gs to adenylate cyclase.…”

Section: Hcg Secretory Response To Camp and Gnrh In Perifusionmentioning

confidence: 99%

Characterization of Cell Types within a Chorionic Gonadotropin-Secreting, Mechanically Dissociated Human Placental Cell Population in Perifusion

Steele

Currie²,

Yuen³

et al. 1993

Neurosignals

View full text Add to dashboard Cite

A cell perifusion system was established to examine human placental endocrine regulation by locally synthesized peptides. First-trimester and term trophoblast cells were mechanically dissociated. Cells were plated on microcarrier beads and cultured for 7–14 days. Cells on beads were loaded in chambers, perifused with culture media and effluent was assayed for chorionic gonadotropin (hCG). Mechanical dissociation of placental tissue produced cell preparations with 85–95% viability. Staining with Masson trichrome, cytokeratin and β-hCG antibodies suggested that greater than 50% of the cells were trophoblast. Perifused trophoblast cells secreted hCG in a continuous non-pulsatile fashion, independent of exogenous hormonal stimuli. hCG secretion from first-trimester trophoblast cells remained stable in static culture for 14 days. GnRH perifusion (10^–8M) for 15–120 s transiently increased hCG secretion from first-trimester trophoblast cells. Longer GnRH exposure stimulated greater hCG secretion. Each of 3 consecutive pulses of 8-bromo-cyclic adenosine-3′,5′-monophosphate (cAMP, 10^–9M) administered at 2-hour intervals stimulated transient hCG secretion from first-trimester and term placental cells. cAMP stimulated hCG secretion more potently from first-trimester than from term placental cells.

show abstract

Particulate guanylate cyclase and adenylate cyclase activities after activation with various agents in rabbit platelets. An ultracytochemical study

1991

View full text Add to dashboard Cite

The cytochemical localization of particulate guanylate cyclase and adenylate cyclase activities in rabbit platelets were studied after stimulation with various agents, at the electron microscope level. In the presence of platelet aggregating agents such as thrombin and ADP, the particulate reaction product of guanylate cyclase activity was detectable on plasma membrane and on membranes of the open canalicular system. In contrast, samples incubated with platelet-activating factor showed no activation of the cyclase activity. Atrial natriuretic factor stimulated the particulate guanylate cyclase. The ultracytochemical localization of this activated cyclase was the same as that of thrombin- or ADP-stimulated guanylate cyclase. Adenylate cyclase activity was studied in platelets incubated with prostaglandin E1 plus or minus insulin. The enzyme reaction product was found at the same sites where guanylate cyclase was detected. Therefore guanylate and adenylate cyclase activities do not seem to be preferentially localised in platelet membranes.

show abstract

Ultracytochemical localization of adenylate cyclase in early human placenta

Cited by 7 publications

References 18 publications

Ultracytochemical localization of particulate guanylate cyclase after stimulation with natriuretic peptides in lamb olfactory mucosa

Ultracytochemical localization of particulate guanylate cyclase after stimulation with natriuretic peptides in lamb olfactory mucosa

Characterization of Cell Types within a Chorionic Gonadotropin-Secreting, Mechanically Dissociated Human Placental Cell Population in Perifusion

Particulate guanylate cyclase and adenylate cyclase activities after activation with various agents in rabbit platelets. An ultracytochemical study

Contact Info

Product

Resources

About