Ultracytochemical Studies in the Dorsal Root and the Dorsal Root Ganglion Neuron of the Rat Spinal Cord

Inomata, Kenichirou; Ogawa, Kazuo

doi:10.1267/ahc.13.359

Cited by 11 publications

(7 citation statements)

References 12 publications

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“…It has been also demonstrated that AcPase accumulation of transected rat sciatic nerve and AcPase activity in the proximal stump exceeded than that in the distal stump (7, 10). Inomata, et al have reported that AcPase activity could be detected on the axolemma of neuron in dorsal root ganglion (9). From these results, it is suggested that the AcPase activity detected in processes of neurons is possibly transported from cell body to process.…”

Section: Observation Of Purkinje Cells Of Cerebellummentioning

confidence: 94%

Ultracytochemical study on nematolysosomes in neurons of the central nervous system.

Shi

Sakai

Ogawa

1992

Acta Histochem. Cytochem.

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In this study, the presence of elongated thread-like lysosomes (nematolysosomes) was demonstrated cytochemically in large pyramidal cell of cerebrum, Purkinje cell of cerebellum and motor neuron in anterior horn of spinal cord when these cells were fixed with low concentrated glutaraldehyde fixatives in PIPES buffer. The acid phosphatase (AcPase; one of lysosomal marker enzymes) activity was mainly distributed in condensed vacuoles and stacks of well-developed Golgi apparatus and in lysosomes in the neurons. However, we have also found that AcPase activity was present not only on ordinary round lysosomes but also on the nematolysosomes. The nematolysosomes with positive AcPase activity were observed in the cytoplasm of the neurons of the central nervous system. The motor neurons have more nematolysosomes which may extend into the processes. In these cells there were many microtubules and microfilaments. This may suggest that the nematolysosomes are related to the AcPase transport in the neurons.

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Section: Observation Of Purkinje Cells Of Cerebellummentioning

confidence: 94%

Ultracytochemical study on nematolysosomes in neurons of the central nervous system.

Shi

Sakai

Ogawa

1992

Acta Histochem. Cytochem.

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“…Gobel et al (7) described that a glomerulus consists of up to nine processes. In the cytochemical investigation, TMPase activity is transported from the dorsal root ganglion to the plasma membrane of the synaptic glomeruli (14)(15)(16). It is suggested that there may be some relationship between the TMPase activity and the pain mechanism in the spinal cord (15).…”

Section: Discussionmentioning

confidence: 99%

“…TMPase activity was transported from the smalltype neuron in the dorsal root ganglion to the plasma membrane of the DSA terminal in the substantia gelatinosa passes through the dorsal root (14)(15)(16).…”

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confidence: 99%

High voltage electron microscopical analysis of synaptic glomeruli in the rat substantia gelatinosa.

Nasu

Chiba

Inomata

et al. 1990

Acta Histochem. Cytochem

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“…small ganglion cells had several unique characteristics. TMPase was localized in the rat small ganglion cells which do not produce typical secretion granules (9,13). The TTN and afferent terminals, which have been supposed to be involved in synaptic transmission (12), displayed intense TMPase activity (9), but the lysosomes were TMPase-negative (9,13).…”

Section: Tppase Activitymentioning

confidence: 99%

“…Thiamine monophosphatase (TMPase) is specifically localized on the reticular part of the Golgi complex in small ganglion cells and in the plasma membrane of electron-dense sinusoid axon terminals of the substantia gelatinosa in the spinal cord (8,9,13). Small ganglion cells elicit small unmyelinated C fibers, known to be involved in the polymodal receptor.…”

mentioning

confidence: 99%

High-voltage electron microscopic observation of golgi complex in the rat small ganglion cell. Thiamine metabolism.

Tomiyasu

Nasu

Inomata

et al. 1990

Acta Histochem. Cytochem

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Thiamine mono-and pyrophosphatase (TMPase, TPPase) activities were demonstrated in the rat small ganglion cell under high-voltage electron microscopy. From a series of micrographs of the same field tilted in increments, a trans tubular network (TTN) consisting of looping and anastomosing tubules was found; this network showed relatively higher intense TMPase activity, and exhibited connections with TMPase-positive vesicle-like bodies. TPPase activity was localized in the trans Golgi saccules, and the TPPase-positive transmost saccule had a structural resemblance to the TTN.These results indicate that the TTN plays an important role in thiamine metabolism.Thiamine monophosphatase (TMPase) is specifically localized on the reticular part of the Golgi complex in small ganglion cells and in the plasma membrane of electron-dense sinusoid axon terminals of the substantia gelatinosa in the spinal cord (8, 9, 13). Small ganglion cells elicit small unmyelinated C fibers, known to be involved in the polymodal receptor. Peripheral C fibers ramify as the naked nerve endings in the peripheral organs, whereas the dorsal root C fibers mainly terminate in the substantia gelatinosa. The substantia gelatinosa is considered to serve as the central control for pain impulses (12, 23); therefore, TMPase activity has proved to be a useful marker for the study of the functions of small ganglion cells (10, 13).Thiamine pyrophosphatase (TPPase), which hydrolizes thiamine pyrophosphate (TPP) into thiamine monophosphate (TMP), has been ultracytochemically shown to be a marker enzyme for the Golgi complex (2, 16). The present study was to clarify the three-dimensional aspects of the TMPase-positive structure, and to compare the distribution of both enzymes under high-voltage electron microscopy. MATERIALS AND METHODSNormal male Wistar rats, each weighing 250-300g, were intraperitoneally anesthetized with sodium pentobarbiturate (50mg/kg) and perfused through the heart with cold 30mM PIPES buffer containing 1% paraformaldehyde and 1% glutaraldehyde, pH 7.3, for 15min. Dorsal root ganglia (DRG) in the lumbar region 127

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Ultracytochemical Studies in the Dorsal Root and the Dorsal Root Ganglion Neuron of the Rat Spinal Cord

Cited by 11 publications

References 12 publications

Ultracytochemical study on nematolysosomes in neurons of the central nervous system.

Ultracytochemical study on nematolysosomes in neurons of the central nervous system.

High voltage electron microscopical analysis of synaptic glomeruli in the rat substantia gelatinosa.

High-voltage electron microscopic observation of golgi complex in the rat small ganglion cell. Thiamine metabolism.

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