Ultrafast Determination of Antimicrobial Resistant Staphylococcus aureus Specifically Captured by Functionalized Magnetic Nanoclusters

Abstract

Sepsis, the systemic response to infection, is a life-threatening situation for patients and leads to high mortality, especially when caused by antimicrobial resistant pathogens. Prompt diagnosis and identification of the pathogenic bacteria, including their antibiotic resistance, are highly desired to yield a timely decision for treatment. Here, we aim to develop a platform for rapid isolation and efficient identification of Staphylococcus aureus, the most frequently occurring pathogen in sepsis. A peptide (VPHNPGLISLQG, SA5-1), specifically binding to S. aureus, was conjugated to the PEGylated magnetic nanoclusters, successfully enabling the specific capture and enrichment of S. aureus from blood serum. Consequently, fast detection of the antimicrobial resistance of the collected S. aureus was achieved within 30 min using a novel luminescent probe. These magnetic nanoclusters manifest a promising diagnostic prospect to combat sepsis.

A rapid and specific antimicrobial resistance detection of Escherichia coli via magnetic nanoclusters

“…All the measured MNCs suspensions were diluted to a final concentration of 0.9 g L −1 and were briefly sonicated for 30 s before each analysis. 28,35,36…”

“…All the measured MNCs suspensions were diluted to a final concentration of 0.9 g L −1 and were briefly sonicated for 30 s before each analysis. 28,35,36 TEM analysis TEM analysis was carried out by exploiting a JEOL TEM equipped with an in-column Omega-type energy filter (JEM-2200FS, Joel, Japan). [37][38][39][40][41][42][43][44] 5 μL of the sample suspension after a 100-fold dilution was depleted to a TEM grid (Carbon Film Supported Copper Grid, 200 Meshes, Electron Microscopy Sciences, USA) till the solvent was fully evaporated.…”

Applications of peptides in nanosystems for diagnosing and managing bacterial sepsis

“…PEGylation also reduces non-specific binding and potential immunogenicity, making the magnetic nanoparticles more suitable for various biomedical applications such as diagnosis and targeted drug delivery [138]. On this point, Pan et al developed a peptide-modified PEGylated-iron oxide composite nanoclusters (peptide@ PEG@MNCs) to isolate and identify S. aureus form blood samples [124]. The peptide (SA5-1: VPHNPGLISLQG) was chosen from a bacteriophage display library for its ability to selectively attach to the cell surface of S. aureus.…”

Specific capture of Pseudomonas aeruginosa for rapid detection of antimicrobial resistance in urinary tract infections

“…TEM analysis was executed through the utilization of a JEOL TEM equipped with an in-column Omega-type energy filter (JEM-2200FS, Joel, Japan) (Wu et al, 2021(Wu et al, , 2022a(Wu et al, , 2022bPan et al, 2016Pan et al, , 2017Pan et al, , 2022aGuo et al, 2022). 5 μL of the sample after a 100-fold dilution was added to a TEM grid (Kung et al, 2018(Kung et al, , 2020Tseng et al, 2020) (Carbon Film Supported Copper Grid, 200 Meshes, Electron Microscopy Sciences, USA) till the solvent was completely evaporated (Yang et al, 2022;Pan et al, 2022b;Yu et al, 2021;Kung et al, 2021).…”