Ultrafast Extraction of Proteins from Tissues Using Desorption by Impulsive Vibrational Excitation

Kwiatkowski, Marcel; Wurlitzer, M.; Omidi, Maryam; Ren, Lu; Kruber, Sebastian; Nimer, Refat M.; Robertson, Wesley D.; Horst, Andrea Kristina; Miller, R. J. Dwayne; Schlüter, Hartmut

doi:10.1002/anie.201407669

Cited by 48 publications

(57 citation statements)

References 23 publications

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“…Multiply charged ions can also be produced with matrix and vacuum alone without the use of a laser, with a reflective geometry, or with a laser in a transmission geometry . A picosecond infrared laser system was recently used to extract and liberate highly charged proteins in water . Ammonium formate and ammonium sulfate have been used to enhance the MALDI sensitivity for lipids and hydrophilic quaternary ammonium compounds, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…34 A picosecond infrared laser system was recently used to extract and liberate highly charged proteins in water. 35,36 Ammonium formate 37 and ammonium sulfate 38 have been used to enhance the MALDI sensitivity for lipids and hydrophilic quaternary ammonium compounds, respectively. Addition of concentrated nitrilotriacetic acid to the matrix solution during sample preparation was also shown to effectively reduce the MALDI matrix-induced ISE and enhance the peptide and protein identification from peptide mass fingerprinting.…”

Section: Introductionmentioning

confidence: 99%

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Characterizing and alleviating ion suppression effects in atmospheric pressure matrix‐assisted laser desorption/ionization

Cao

Liu

et al. 2019

Rapid Comm Mass Spectrometry

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RATIONALE: As a powerful ambient ion source, atmospheric pressure (AP)-MALDI enables direct analysis at atmospheric pressure/temperature and minimal sample preparation. With the increasing usage of AP-MALDI sources with Orbitrap instruments, systematic characterization of the extent of ion suppression effect (ISE) in AP-MALDI-Orbitrap mass spectrometry imaging (MSI) is desirable. Recently, a new low-pressure MALDI platform has been introduced that reportedly provided better sensitivity. While extensive research efforts have been devoted to improving spatial resolution, fewer studies focused on the characterization and sensitivity improvement of these MALDI platforms that, coupled with high-resolution Orbitraps, provide powerful strategy for MS imaging. METHODS: Here, we compared the analytical performance of AP and low-pressure (subatmospheric) MALDI sources to study the effect of pressure control in the ion source. Using a model peptide/protein mixture, we systematically evaluated the factors influencing ISE. Furthermore, the effect of laser spot size was evaluated through tissue imaging analysis of lipids and neuropeptides. The effects of ion suppression and laser spot size have also been examined by comparing the number of identified molecular species during MSI analysis. RESULTS: Several key operating parameters including source pressure, laser energy, laser repetition rate, and microscopic slide coating materials were optimized to minimize the ISE. Under the optimal conditions, the subatmospheric AP-MALDI-Orbitrap platform with high spatial and mass spectral resolution enabled significantly improved coverage of several lipid and neuropeptide families in the MS analysis of mouse brain tissue sections. CONCLUSIONS: The new SubAP-MALDI source coupled with an Orbitrap mass spectrometer was established as a viable platform for in situ endogenous biomolecular analysis with increased sensitivity compared to conventional AP-MALDI sources as evidenced by the confident identification of neuropeptides from mouse brain imaging analyses. The alleviated ISE was key to substantial performance improvement due to optimized intermediate pressure conditions and better ion collection by the ion funnel.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterizing and alleviating ion suppression effects in atmospheric pressure matrix‐assisted laser desorption/ionization

Cao

Liu

et al. 2019

Rapid Comm Mass Spectrometry

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“…The method has been particularly applicable as a surgical technique with minimized collateral damage to surrounding tissue (Amini-Nik et al, 2010;Petersen et al, 2016). Recently, we have demonstrated PIRL-DIVE ablation for the soft extraction of structurally and even functionally intact biomolecular complexes such as large protein complexes ($520 kDa) and viruses ($40 MDa) as well as unmodified protein species, directly from aqueous solution and tissue (Kwiatkowski et al, 2015(Kwiatkowski et al, , 2016Ren et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Protein crystals IR laser ablated from aqueous solution at high speed retain their diffractive properties: applications in high-speed serial crystallography

et al. 2017

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In order to utilize the high repetition rates now available at X-ray free-electron laser sources for serial crystallography, methods must be developed to softly deliver large numbers of individual microcrystals at high repetition rates and high speeds. Picosecond infrared laser (PIRL) pulses, operating under desorption by impulsive vibrational excitation (DIVE) conditions, selectively excite the OH vibrational stretch of water to directly propel the excited volume at high speed with minimized heating effects, nucleation formation or cavitationinduced shock waves, leaving the analytes intact and undamaged. The soft nature and laser-based sampling flexibility provided by the technique make the PIRL system an interesting crystal delivery approach for serial crystallography. This paper demonstrates that protein crystals extracted directly from aqueous buffer solution via PIRL-DIVE ablation retain their diffractive properties and can be usefully exploited for structure determination at synchrotron sources. The remaining steps to implement the technology for high-speed serial femtosecond crystallography, such as single-crystal localization, high-speed sampling and synchronization, are described. This proof-of-principle experiment demonstrates the viability of a new laser-based high-speed crystal delivery system without the need for liquid-jet injectors or fixed-target mounting solutions

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“…At the same time, efficient energy redistribution into thermal modes is ensured by keeping the irradiance low enough to avoid nonlinear optical excitation of higher-lying molecular states. Picosecond infrared laser (PIRL) irradiation fulfilling these criteria has been shown to be well-suited for the extraction of large bio-molecules and even intact viruses for subsequent offline analysis, [20][21][22] as an atmospheric online ion source in combination with electrospray ionization (LAESI), 23 and has been suggested as a potential tool for sample-delivery in high-speed X-ray crystallography. 24 Moreover, the absence of ionizing radiation and minimal deposition of thermal and acoustic energy into the non-ablated sample surface make DIVE a valuable tool for precise material removal in surgical applications.…”

Section: Introductionmentioning

confidence: 99%

Digital interference microscopy and density reconstruction of picosecond infrared laser desorption at the water-air interface

Busse

Kruber

Robertson

et al. 2018

Journal of Applied Physics

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Material ablation and evaporation using pulsed infrared lasers pose promising approaches for matrix-free laser desorption ionization and in laser surgery. For the best results, key parameters such as laser wavelength, pulse duration, and pulse energy need to be carefully adjusted to the application. We characterize the dynamics at the water-air interface induced by a 10 ps infrared laser tuned to the water absorption band at 3 lm, a parameter set facilitating stress confined desorption for typical absorption depths in biological samples and tissue. By driving the ablation faster than nucleation growth, cavitation induced sample damage during the ablation process can be mitigated. The resultant explosive ablation process leads to a shock front expansion and material ejection which we capture using off-axis digital interference microscopy, an interference technique particularly useful for detecting the phase shift caused by transparent objects. It is demonstrated that the method can yield local density information of the observed shock front with a single image acquisition as compared to the usually performed fit of the velocity extracted from several consecutive snapshots. We determine the ablation threshold to be ð0:560:2Þ J cm À2 and observe a significant distortion of the central parts of the primary shock wave above approximately 2:5 J cm À2. The differences in plume shape observed for higher fluences are reflected in an analysis based on shock wave theory, which shows a very fast initial expansion.

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Ultrafast Extraction of Proteins from Tissues Using Desorption by Impulsive Vibrational Excitation

Cited by 48 publications

References 23 publications

Characterizing and alleviating ion suppression effects in atmospheric pressure matrix‐assisted laser desorption/ionization

Characterizing and alleviating ion suppression effects in atmospheric pressure matrix‐assisted laser desorption/ionization

Protein crystals IR laser ablated from aqueous solution at high speed retain their diffractive properties: applications in high-speed serial crystallography

Digital interference microscopy and density reconstruction of picosecond infrared laser desorption at the water-air interface

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