2024
DOI: 10.1002/cpz1.70006
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Ultrafast His‐Tagged Protein Purification

Xuan Luo,
Arjun S. Pamidi,
Zoe Gardner
et al.

Abstract: This article details how to use a vortex fluidic device (VFD) to accelerate protein purification via immobilized metal affinity chromatography (IMAC). Building upon a previous report of VFD‐based purification, we introduce a membrane insert to simplify the purification protocol and the resin recovery step. This new platform can be adapted to different types of IMAC resins and purification membranes. Proteins can be purified directly from clarified lysate, non‐clarified lysate, and even non‐lysed cultures witho… Show more

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