Ultrafiltration,
an advanced process for producing biologically
safe reclaimed water, has confronted challenges from bacterial regrowth.
We conducted a comprehensive investigation into the biosafety risks
of ultrafiltration-treated reclaimed water under five distinct storage
conditions, considering temperature variations, airborne bacterial
invasion, and light. Although ultrafiltration removed over 97% of
the bacterial load, bacterial regrowth occurred during storage. Elevated
temperatures resulted in the highest heterotrophic plate count (HPC),
while lower temperatures favored increased total cell count (TCC).
Notably, lower temperatures facilitated a surge in the number of potential
pathogenic bacteria. Light exposure also elevated the potential pathogenic
bacteria. Surprisingly, high temperatures posed minimal biosafety
risk. While airborne bacterial invasion insignificantly affected potential
pathogenic bacterial count, it constituted 67 ± 4% of total regrowth
bacteria, with membrane breakthrough bacteria comprising the remaining
33 ± 4%, as revealed by SourceTracker. The impact of storage
conditions on the bacterial community structure varied, with temperature
exerting the most significant effect. Correlation analysis affirmed
the potential of UV254 and total fluorescence intensity
as alternative indicators for HPC, and polysaccharide content emerged
as a suitable substitute indicator for potential pathogenic bacterial
count. Future endeavors should prioritize the development of integrated
ultrafiltration processes designed to inhibit pathogen regrowth in
reclaimed water treatment.