Ultrahochfeld-MRT am Hühnerembryo in ovo – ein Modell für die experimentelle Ophthalmologie

Klose, Ronja; Streckenbach, Felix; Hadlich, Stefan; Stahnke, Thomas; Guthoff, Rudolf; Wree, Andreas; Frank, Marcus; Langner, Sönke; Stachs, Oliver; Lindner, Tobias

doi:10.1055/s-0043-120675

Cited by 7 publications

(8 citation statements)

References 22 publications

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“…Chicken embryos usually hatch after 21 days and ostrich embryos after 42 days, which contributes to rather simple translation of development stages (Gefen & Ar, 2001). The embryonal structures of chicken eggs are too small to be depicted on regular scanners used in clinical routine with humans; therefore, they have been investigated using dedicated small animal imaging devices as micro‐CT, ‐MRI, ‐SPECT and –PET (or the combination thereof) (Bain et al., 2007; Gebhardt et al., 2013; Heidrich et al., 2011; Klose et al., 2017; Kulesa et al., 2010; Wurbach et al., 2012). Shojaei et al (2014) reported on the use of a regular MRI‐scanner (1.5 T) with ostrich eggs, investigating first embryonal stages until DD 18 and they found a very good soft tissue contrast, enabling delineation of the eye ball as early as DD 10.…”

Section: Discussionmentioning

confidence: 99%

In‐ovo imaging using ostrich eggs—Evaluation of physiological embryonal development on computed tomography

et al. 2021

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Section: Discussionmentioning

confidence: 99%

In‐ovo imaging using ostrich eggs—Evaluation of physiological embryonal development on computed tomography

et al. 2021

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“…Therefore, after this embryonic stage, the eggs were placed on crushed ice starting 10 min prior to MR imaging. Temperature was monitored during the entire scanning time using a fibre‐optical thermometer (1025T, Monitoring & Gating system, Small Animal Instruments) as described previously (Klose et al, 2017; Streckenbach et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

“…The position of the embryo within the egg was identified using a Gating system, Small Animal Instruments) as described previously (Klose et al, 2017;Streckenbach et al, 2018).…”

Section: Mr Imagingmentioning

confidence: 99%

“…In recent studies, we demonstrated that MRM is capable of evaluating the development of the chicken in ovo through the entire development period without affecting normal development (Klose et al, 2017; Streckenbach et al, 2018). Our recent studies focussed especially on the development of the eyes (Klose et al, 2017; Lindner et al, 2017; Streckenbach et al, 2018). Therefore, we want to use the established model of chicken embryos to investigate to what extent other organ systems can be assessed using MRM.…”

Section: Introductionmentioning

confidence: 99%

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MR microscopy of the developing upper extremity of the chicken in ovo using 7 Tesla MRI

Streckenbach

Jäschke

Jahns

et al. 2022

Anat Histol Embryol

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MR microscopy (MRM) is known as ultra‐high‐field (UHF) magnetic resonance imaging with an in‐plane spatial resolution of <100 μm, yields highly resolved non‐invasive anatomical imaging and allows longitudinal assessment of embryonic avian development. The aim of the present study was to evaluate the feasibility of in vivo anatomical MRI assessment of the developing upper extremity of the chicken. Thirty‐eight fertilized chicken eggs were examined at 7 Tesla acquiring high‐resolution T2‐weighted images with an in‐plane resolution of 74 × 74 μm. To reduce motion artefacts, the eggs were moderately cooled before and during MRI. Development of the upper extremity was anatomically and quantitatively assessed. Chondrification and ossification on MRI were correlated with histological examination. MRM allowed the identification of the embryo from stage D5 onwards. First chondrification of the upper extremity was visible at stage D7, and the differentiation of the forearm was possible from stage D9 throughout the developmental period with excellent correlation to histology. MRM also allowed the differentiation between cortical and medullary bone as well as the detection of chondrified areas. UHF MRM allows the in vivo and in ovo evaluation of the upper limb during embryonic development and provides non‐invasive longitudinal anatomical information. This technique allows longitudinal studies of the same embryo during the developmental period and may therefore provide further insights into the development of the upper extremity. With improved coil technique and increasing availability of UHF MR systems, there is great potential regarding several research topics in experimental musculoskeletal radiology.

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“…The undifferentiated neurons in the visual system of a chick embryo start to differentiate into neurons of optic vesicles on embryonic day (ED) 2, and axons from retinal ganglion cells reach the optic chiasma by ED4 (78) (Figure 5). The ocular compartments can be identified at ED4 (81). Eye formation is completed, and long-wavelength photo pigments are expressed, at ED14.…”

Section: Effects Of Light During Incubationmentioning

confidence: 99%

Prenatal and Early Postnatal Behavioural Programming in Laying Hens, With Possible Implications for the Development of Injurious Pecking

et al. 2021

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Injurious pecking (IP) represents a serious concern for the welfare of laying hens (Gallus gallus domesticus). The risk of IP among hens with intact beaks in cage-free housing prompts a need for solutions based on an understanding of underlying mechanisms. In this review, we explore how behavioural programming via prenatal and early postnatal environmental conditions could influence the development of IP in laying hens. The possible roles of early life adversity and mismatch between early life programming and subsequent environmental conditions are considered. We review the role of maternal stress, egg conditions, incubation settings (temperature, light, sound, odour) and chick brooding conditions on behavioural programming that could be linked to IP. Brain and behavioural development can be programmed by prenatal and postnatal environmental conditions, which if suboptimal could lead to a tendency to develop IP later in life, as we illustrate with a Jenga tower that could fall over if not built solidly. If so, steps taken to optimise the environmental conditions of previous generations and incubation conditions, reduce stress around hatching, and guide the early learning of chicks will aid in prevention of IP in commercial laying hen flocks.

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Ultrahochfeld-MRT am Hühnerembryo in ovo – ein Modell für die experimentelle Ophthalmologie

Cited by 7 publications

References 22 publications

In‐ovo imaging using ostrich eggs—Evaluation of physiological embryonal development on computed tomography

In‐ovo imaging using ostrich eggs—Evaluation of physiological embryonal development on computed tomography

MR microscopy of the developing upper extremity of the chicken in ovo using 7 Tesla MRI

Prenatal and Early Postnatal Behavioural Programming in Laying Hens, With Possible Implications for the Development of Injurious Pecking

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