The aim of this work is synthesis a novel nanocomposite containing Polylactide (PLA) and polyamide 6 (PA6) reinforced with graphene oxide (GO) and poly ethylene-butyl acrylate-glycidyl methacrylate) (PTW) compatibilizer during solvent-based method. For this purpose, GO was added to the nanocomposite with 0.1, 0.3, 0.5, 0.7 and 1 phr. Morphology, rheology and mechanical properties of nanocomposites were studied with scanning electron microscopy (SEM), transmission electron microscopy (TEM) and (DMTA) which showed rougher fracture surface due to the presence of compatibilizer and an increase in the amount of graphene oxide and better dispersion of graphene oxide. The results of experimental and theoretical studies of mechanical properties showed that increasing the concentration of graphene oxide in the presence of PTW improved the tensile strength, impact strength and tensile modulus in the PA6/PTW/PLA system. The study of rheological properties (according to the Carreau-Yasuda model) showed an increase in storage modulus and complex viscosity, which also confirmed the role of PTW compatibilizer in better GO dispersion. So, PA6/PTW/PLA is a good candidate for mechanical and high thermal applications.