Ultramorphological characteristics of immature stages of a forensically important fly Parasarcophaga ruficornis (Fabricius) (Diptera: Sarcophagidae)

Singh, Devinder; Garg, Rashmi; Wadhawan, Bhanvi

doi:10.1007/s00436-011-2561-7

Cited by 19 publications

(35 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…cultellata differs more from the second and third larval stages than these two stages from each other. The morphological features of the larva‐body may evolve throughout the immature life cycle in two ways, by (a) retaining both their basic structure and arrangement or (b) becoming increasingly complex and completely changing their appearance (e.g., Draber‐Monko et al, ; Grzywacz et al, ; Paños et al, ; Singh et al, ; Sukontason et al, , ; Ubero‐Pascal et al, ). The segmentary pattern of the spinose‐bands and sensilla ring, the arrangement of the sensilla in the maxillary palpus or posterior papillae around the spiracular cavity, and the shape of the peristigmatic tufts, among others structures, are maintain features that allow the specific correlation of all immature stages.…”

Section: Discussionmentioning

confidence: 99%

“…As regarding SEM descriptions, the sarcophagids have been barely and unevenly studied: the first instar larvae of about 70 European Miltogramminae species is known (Szpila, ; Szpila and Pape, , 2007, ); among the Paramachronychiinae, all the larval stages of Wohlfahrtia magnifica (Schiner, 1862) and first instar larvae of Sarcophila latifrons (Fallén, 1817) are known (Ruíz‐Martínez et al, ; Szpila, ); and from Sarcophaginae, the best studied genus is Sarcophaga , although several non‐Palaeartic species belonging to other genera have also been described (Leite and Lopes, ; Lopes and Leite, ; Mendoça et al, ). With regards to Sarcophaga , three species have been partially described (Aspoas, ), four species have descriptions of third instar larva stage alone (Kirk‐Spriggs, ; Pérez‐Moreno et al, ), and all the immature stages have been described for three more species (Awad et al, ; Draber‐Monko et al, ; Singh et al, ; Sukontason et al, , 2006; Suwannayod et al, ). Finally, some unidentified larvae have also been described (Colwell and O'Connor, ; Sukontason et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…( L .) ruficornis have been studied by both microscopy techniques (Alfred, ; Awad et al, ; Draber‐Monko et al, ; Hafez, ; Knipling, ; Richet et al, ; Singh et al, ; Sukontason et al, ; Suwannayod et al, ; Yates, ), whereas S . ( L .)…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Micromorphology of immature stages of Sarcophaga (Liopygia) cultellataPandellé, 1896 (Diptera: Sarcophagidae), a forensically important fly

Ubero-Pascal

Paños

García

et al. 2014

Microscopy Res & Technique

View full text Add to dashboard Cite

The Sarcophagidae are one of the most numerous groups of Diptera in the world, consisting of many species of forensic interest, whose immature stages are useful in the estimation of postmortem interval. The immature stages of some species of forensic importance still remain unknown or undescribed, like in the case of Sarcophaga (Liopygia) cultellata Pandellé, 1986, a species restricted to the Iberian Peninsula, south of France and north of Italy, which shares a ecological niche with species of the same subgenus, e.g., Sarcophaga (Liopygia) argyrostoma (Robineau-Desvoidy, 1830) and Sarcophaga (Liopygia) crassipalpis Macquart, 1839, making it necessary to lay the groundwork for a proper specific differentiation before it can be successfully applied in forensic practice. This study provides the first micromorphological description of all preimaginal stages of S. (L.) cultellata using light microscopy and scanning electron microscopy (SEM), the results of which allow the morphology of the main features to be followed during the immature life cycle. We propose a combination of features for distinguishing Liopygia from other sarcophagid subgenera, based on the current level of morphological knowledge of immature stages. S. (L.) cultellata can be differentiated from S. (L.) argyrostoma and S. (L.) crassipalpis in every immature stage by both light microscopy and SEM. The presence of tegumental warts and a fan-shaped anterior spiracle with a single row of 15-18 respiratory papillae allow distinguishing the third instar larvae of S. (L.) cultellata from other Sarcophaga species described hitherto by SEM. Identification keys based on light microscopy observations are provided, covering all the immature stages of Liopygia subgenus occurring in the Iberian Peninsula.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Micromorphology of immature stages of Sarcophaga (Liopygia) cultellataPandellé, 1896 (Diptera: Sarcophagidae), a forensically important fly

Ubero-Pascal

Paños

García

et al. 2014

Microscopy Res & Technique

View full text Add to dashboard Cite

show abstract

“…The morphology of maxillary palp complex is considered to be of help in interspecific identification (Kirk‐Spriggs,2003; Singh et al,2012). The maxillary palp complex of D .…”

Section: Discussionmentioning

confidence: 99%

A study on morphology of immature stages of Diplonevra peregrina (Wiedemann) (Diptera: Phoridae)

Feng

Liu

2012

Microscopy Res & Technique

View full text Add to dashboard Cite

Morphology of all larval instars and puparium of Diplonevra peregrina, a most common phorid fly species indoors in China, is presented using scanning electron microscopy. The first instar larva was composed of 12 segments, each of segments 3-11 with six spicate tubercles situated dorsally, dorsolaterally, and laterally in transverse row. The dorsal tubercles were much longer than the laterals and dorsolaterals. Antennae and maxillary palp complex were visible. The caudal segment was margined by six long, stout tubercles covered by numerous long bristles at the base through the apex. Two slits could be seen at the posterior spiracle. Besides the presence of anterior spiracle, the tubercles of second instar became more stout than those of first instar and were covered by numerous long bristles from the base to top. The posterior spiracle contained four slits. Third larval instar was similar to second instar. The bubble membrane comprised of clusters of small spines presented at the segment 5 of third instar larvae. Puparia showed a retracted cephalic region and a pair of pupal respiratory horns on the dorsum.

show abstract

“…This is very time-consuming and requires living larvae. Other methods based on light microscopy rely on the morphological characteristics of the larvae, like the shape of the cephalopharyngeal skeleton (CPS), spiracles, or spinebands [13,14,15,16,17,18,19,20,21,22]. These methods can be hindered by the fragmentation or discoloration of the specimen by poor preservation.…”

Section: Introductionmentioning

confidence: 99%

Connecting the Dots: From an Easy Method to Computerized Species Determination

Niederegger

Döge

Marynen

et al. 2017

Insects

View full text Add to dashboard Cite

Differences in growth rate of forensically important dipteran larvae make species determination an essential requisite for an accurate estimation of time since colonization of the body. Interspecific morphological similarities, however, complicate species determination. Muscle attachment site (MAS) patterns on the inside of the cuticula of fly larvae are species specific and grow proportionally with the animal. The patterns can therefore be used for species identification, as well as age estimation in forensically important dipteran larvae. Additionally, in species where determination has proven to be difficult—even when employing genetic methods—this easy and cheap method can be successfully applied. The method was validated for a number of Calliphoridae, as well as Sarcophagidae; for Piophilidae species, however, the method proved to be inapt. The aim of this article is to assess the utility of the MAS method for applications in forensic entomology. Furthermore, the authors are currently engineering automation for pattern acquisition in order to expand the scope of the method. Automation is also required for the fast and reasonable application of MAS for species determination. Using filters on digital microscope pictures and cross-correlating them within their frequency range allows for a calculation of the correlation coefficients. Such pattern recognition permits an automatic comparison of one larva with a database of MAS reference patterns in order to find the correct, or at least the most likely, species. This facilitates species determination in immature stages of forensically important flies and economizes time investment, as rearing to adult flies will no longer be required.

show abstract

Ultramorphological characteristics of immature stages of a forensically important fly Parasarcophaga ruficornis (Fabricius) (Diptera: Sarcophagidae)

Cited by 19 publications

References 45 publications

Micromorphology of immature stages of Sarcophaga (Liopygia) cultellataPandellé, 1896 (Diptera: Sarcophagidae), a forensically important fly

Micromorphology of immature stages of Sarcophaga (Liopygia) cultellataPandellé, 1896 (Diptera: Sarcophagidae), a forensically important fly

A study on morphology of immature stages of Diplonevra peregrina (Wiedemann) (Diptera: Phoridae)

Connecting the Dots: From an Easy Method to Computerized Species Determination

Contact Info

Product

Resources

About