Ultrarapid Engineering of Biomimetic Materials and Tissues: Fabrication of Nano‐ and Microstructures by Plastic Compression

Brown, Robert A.; Wiseman, M.; Chuo, Cher-Bing; Cheema, Umber; Nazhat, Showan N.

doi:10.1002/adfm.200500042

Cited by 426 publications

(534 citation statements)

References 23 publications

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“…Central in this concept is the reduction of the liquid content of the scaffold, which is a result of the casting. 21 Plastic compression therefore yields scaffolds much denser than conventional, uncompressed collagen type I matrices. The moduli of these conventional scaffolds are typically below 1 kPa, 22,23 and these scaffolds should first grow stronger in culture.…”

Section: Discussionmentioning

confidence: 99%

“…18 In this study, we used a plastic compression technique to develop collagen I-based scaffolds with varying concentrations and viscoelastic properties. This is a novel technique that was first described by Brown et al 21 as a form of cell-independent engineering. Central in this concept is the reduction of the liquid content of the scaffold, which is a result of the casting.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement

Bron

Koenderink

Everts

et al. 2008

Journal Orthopaedic Research

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Lumbar discectomy is an effective therapy for neurological decompression in patients suffering from sciatica due to a herniated nucleus pulposus (NP). However, high numbers of patients suffering from persisting postoperative low back pain have resulted in many strategies targeting the regeneration of the NP. For successful regeneration, the stiffness of scaffolds is increasingly recognized as a potent mechanical cue for the differentiation and biosynthetic response of (stem) cells. The aim of the current study is to characterize the viscoelastic properties of the NP and to develop dense collagen scaffolds with similar properties. The scaffolds consisted of highly dense (0.5%-12%) type I collagen matrices, prepared by plastic compression. The complex modulus of the NP was 22 kPa (at 10 rad s À1 ), which should agree with a scaffold with a collagen concentration of 23%. The loss tangent, indicative of energy dissipation, is higher for the NP (0.28) than for the scaffolds (0.12) and was not dependent of the collagen density. Gamma sterilization of the scaffolds increased the shear moduli but also resulted in more brittle behavior and a reduced swelling capacity. In conclusion, by tuning the collagen density, we can approach the stiffness of the NP. Therefore, dense collagen is a promising candidate for tissue engineering of the NP that deserves further study, such as the addition of other proteins. ß

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement

Bron

Koenderink

Everts

et al. 2008

Journal Orthopaedic Research

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“…While hydrogel stiffness depends on factors such as its concentration and cross-linking, due to its nonlinear behaviour, the stiffness can be altered as a result of internal contractile forces exerted by cells or by another internal compressing and/or stretching forces exerted within it [1,2]. Several approaches have been proposed in the literature to enhance the local stiffness of the hydrogel, including plastic compression [6], cross-linking techniques [3] and magnetic field alignment of collagen-based hydrogels [1]. A helpful alternative approach to remotely induce an internal force within hydrogels and to change their relative local stiffness is to incorporate magnetic nanoparticles within them.…”

Section: Introductionmentioning

confidence: 99%

Numerical modeling of cell differentiation and proliferation in force-induced substrates via encapsulated magnetic nanoparticles

Mousavi

Doweidar

2016

Computer Methods and Programs in Biomedicine

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Cell migration, differentiation, proliferation and apoptosis are the main processes in tissue regeneration. Mesenchymal Stem Cells (MSCs) have the potential to differentiate into many cell phenotypes such as tissue-or organ-specific cells to perform special functions. Experimental observations illustrate that differentiation and proliferation of these cells can be regulated according to internal forces induced within their Extracellular matrix (ECM). The process of how exactly they interpret and transduce these signals is not well understood. Therefore, a previously developed three-dimensional (3D) computational model is here extended and employed to study how force-free substrates (FFS) and force-induced substrate (FIS) control cell differentiation and/or proliferation during the mechanosensing process. Consistent with experimental observations, it is assumed that cell internal deformation (a mechanical signal) in correlation with the cell maturation state directly triggers cell differentiation and/or proliferation. ECM is modeled as Neo-Hookean hyperelastic material assuming that cells are cultured within 3D nonlinear hydrogels. In agreement with wellknown experimental observations, the findings here indicate that within neurogenic (0.1-1 kPa), chondrogenic (20-25 kPa) and osteogenic (30-45 kPa) substrates, MSC differentiation and cell proliferation can be precipitated by inducing the substrate with an internal force. Therefore, cells require a longer time to grow and maturate within force-free substrates than withen force-induced substrates. In the instance of MSC differentiation into a compatible phenotype, the magnitude of the net traction force increases within chondrogenic and osteogenic substrates while it reduces within neurogenic substrates. This is consistent with experimental studies and numerical works recently published by the same authors. However, in all cases the magnitude of the net traction force considerably increases at the instant of cell proliferation because of cell-cell interaction. Consequently, the present model provides new perspectives to delineate the role of force-induced substrates in remotely controlling the cell fate during cell-matrix interaction, which open the door for new tissue regeneration methodologies.

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“…Fibrinencapsulated keratinocytes applied to the surface of Alloderm produce a continuous epithelium with a cornified layer and basement membrane after 4 weeks in vivo (Bannasch et al 2008). Brown et al (2005) and co-workers have shown that plastic compressed collagen gel can be used to encapsulate fibroblasts for tissue engineering the dermis. The compression was only seen to reduce cell viability of encapsulated human dermal fibroblasts by 10% as long as the gel did not become desiccated.…”

Section: Skinmentioning

confidence: 99%

Cell encapsulation using biopolymer gels for regenerative medicine

2010

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To cite this version:Nicola C. Hunt, Liam M. Grover. Cell encapsulation using biopolymer gels for regenerative medicine. Biotechnology Letters, Springer Verlag, 2010, 32 (6), pp.733-742. <10.1007/s10529-010-0221-0>. Abstract There has been a consistent increase in the mean life expectancy of the population of the developed world over the past century. Healthy life expectancy, however, has not increased concurrently. As a result we are living a larger proportion of our lives in poor health and there is a growing demand for the replacement of diseased and damaged tissues. While traditionally tissue grafts have functioned well for this purpose, the demand for tissue grafts now exceeds the supply. For this reason, research in regenerative medicine is rapidly expanding to cope with this new demand. There is now a trend towards supplying cells with a material in order to expedite the tissue healing process. Hydrogel encapsulation provides cells with a three dimensional environment similar to that experienced in vivo and therefore may allow the maintenance of normal cellular function in order to produce tissues similar to those found in the body. In this review we discuss biopolymeric gels that have been used for the encapsulation of mammalian cells for tissue engineering applications as well as a brief overview of cell encapsulation for therapeutic protein production. This review focuses on agarose, alginate, collagen, fibrin, hyaluronic acid and gelatin since they are widely used for cell encapsulation. The literature on the regeneration of cartilage, bone, ligament, tendon, skin, blood vessels and neural tissues using these materials has been summarised.

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Ultrarapid Engineering of Biomimetic Materials and Tissues: Fabrication of Nano‐ and Microstructures by Plastic Compression

Cited by 426 publications

References 23 publications

Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement

Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement

Numerical modeling of cell differentiation and proliferation in force-induced substrates via encapsulated magnetic nanoparticles

Cell encapsulation using biopolymer gels for regenerative medicine

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