Ultrasensitive and selective detection of mercury(II) in aqueous solution by polymerase assisted fluorescence amplification

Zhu, Xiao; Zhou, Xiaoming; Xing, Da

doi:10.1016/j.bios.2010.03.013

Cited by 44 publications

(25 citation statements)

References 39 publications

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“…The addition of Hg 2+ induced the poly-T oligonucleotide to fold into a dsDNA structure, enhancing the fluorescence intensity of TOTO-3. Several groups employed SYBR Green I as a fluorescent reporter in different label-free designs based on T-Hg 2+ -T (79–81). The Chang group (82) developed a fluorescent biosensor for Hg 2+ using T-rich DNA–functionalized AuNPs as a recognition unit and OliGreen as a fluorescent reporter.…”

Section: Biosensors Based On Metal Ion–stabilized Dna Mismatchesmentioning

confidence: 99%

Metal Ion Sensors Based on DNAzymes and Related DNA Molecules

Zhang

Kong

Lu³

2011

Annual Rev. Anal. Chem.

333

252

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Metal ion sensors are an important yet challenging field in analytical chemistry. Despite much effort, only a limited number of metal ion sensors are available for practical use because sensor design is often a trial-and-error-dependent process. DNAzyme-based sensors, in contrast, can be developed through a systematic selection that is generalizable for a wide range of metal ions. Here, we summarize recent progress in the design of DNAzyme-based fluorescent, colorimetric, and electrochemical sensors for metal ions, such as Pb2+, Cu2+, Hg2+, and UO22+ In addition, we also describe metal ion sensors based on related DNA molecules, including T-T or C-C mismatches and G-quadruplexes.

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Section: Biosensors Based On Metal Ion–stabilized Dna Mismatchesmentioning

confidence: 99%

Metal Ion Sensors Based on DNAzymes and Related DNA Molecules

Zhang

Kong

Lu³

2011

Annual Rev. Anal. Chem.

333

252

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“…7,8 Recently, numerous new types of boronic acid sensors have been fabricated to detect blood glucose concentrations, [9][10][11][12][13] ionic compounds, [14][15][16][17] traces of water in solvents, 18,19 hydrogen peroxide (H 2 O 2 ), [20][21][22] catecholamines, 23 as well as using boronic acids as biochemical tools for various applications, including tumor cell imaging, 24,25 enzyme inhibitors 26 and cell delivery systems. 27 Therefore, the boron-based uorescent sensor is still hot in today's research.…”

Section: 5mentioning

confidence: 99%

Recent development of boronic acid-based fluorescent sensors

et al. 2018

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As Lewis acids, boronic acids can bind with 1,2-or 1,3-diols in aqueous solution reversibly and covalently to form five or six cyclic esters, thus resulting in significant fluorescence changes. Based on this phenomenon, boronic acid compounds have been well developed as sensors to recognize carbohydrates or other substances. Several reviews in this area have been reported before, however, novel boronic acid-based fluorescent sensors have emerged in large numbers in recent years. This paper reviews new boronbased sensors from the last five years that can detect carbohydrates such as glucose, ribose and sialyl Lewis A/X, and other substances including catecholamines, reactive oxygen species, and ionic compounds. And emerging electrochemically related fluorescent sensors and functionalized boronic acid as new materials including nanoparticles, smart polymer gels, and quantum dots were also involved.By summarizing and discussing these newly developed sensors, we expect new inspiration in the design of boronic acid-based fluorescent sensors.

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“…SYBR Green I (SG) is commonly used as a reporter in molecular detection because of its favorable photo physical properties, temperature stability, and high sensitivity. 9 Recent studies have revealed that SG showed high selectivity and detection sensitivity toward double-stranded DNA (dsDNA) due to its high enhancement of fluorescence upon interaction with …”

Section: Introductionmentioning

confidence: 99%

Label-free and sensitive fluorescence detection of nucleic acid, based on combination of a graphene oxid /SYBR green I dye platform and polymerase assisted signal amplification

Zhu

Xing

2012

SPIE Proceedings

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A new label-free isothermal fluorescence amplification detection for nucleic acid has been developed. In this paper, we first developed a novel sensitive and specific detection platform with an unmodified hairpin probe (HP) combination of the graphene oxid (GO)/ SYBR green I dye (SG), which was relied on the selective principle of adsorption and the high quenching efficiency of GO. Then for the application of this new strategy, we used Mirco RNA-21 (Mir-21) as the target to evaluate this working principle of our design. When the target was hybridizing with the HP and inducing its conformation of change, an efficient isothermal circular strand-displacement polymerization reaction was activating to assist the first signal amplification. In this format, the formed complex conformation of DNA would interact with its high affinity dye, then detached from the surface of GO after incubating with the platform of GO/intercalating dye. This reaction would accompany with obvious fluorescence recovery, and accomplish farther signal enhancement by a mass of intercalating dye inserting into the minor groove of the long duplex replication product. By taking advantage of the multiple amplification of signal, this method exerted substantial enhancement in sensitivity and could be used for rapid and selective detection of Mir-21 with attomole range. It is expected that this cost-effective GO based sensor might hold considerable potential to apply in bioanalysis studies.

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Ultrasensitive and selective detection of mercury(II) in aqueous solution by polymerase assisted fluorescence amplification

Cited by 44 publications

References 39 publications

Metal Ion Sensors Based on DNAzymes and Related DNA Molecules

Metal Ion Sensors Based on DNAzymes and Related DNA Molecules

Recent development of boronic acid-based fluorescent sensors

Label-free and sensitive fluorescence detection of nucleic acid, based on combination of a graphene oxid /SYBR green I dye platform and polymerase assisted signal amplification

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