Ultrasensitive ATP Detection Using Firefly Luciferase Entrapped in Sugar-Modified Sol−Gel-Derived Silica

Cruz-Aguado, Jorge A.; Chen, Yang; Zhang, Zheng; Elowe, Nadine H.; Brook, Michael A.; Brennan, John D.

doi:10.1021/ja0490424

Cited by 98 publications

(70 citation statements)

References 24 publications

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“…While sugar-modified silica materials have been demonstrated to be suitable hosts for entrapped proteins, and have been shown to be less susceptible to shrinkage and cracking relative to unmodified materials [2] , little has been done with regard to the characterization of the pore environment within such materials. It is known that the presence of organic moieties within sol-gel materials has a great impact on the microenvironment and properties of silica materials [3] .…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Microenvironment characterization for a novel biocompatible hybrid carbon-silicon material

Sui

Zha

et al. 2009

Sci. China Ser. B-Chem.

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The compatible carbon-silicon complex materials originated from precursor diglycerylsilane (DGS) and sugar-modified silane N-(3-triethoxysilylpropyl)gluconamide (GLS) have gained substantial popularity by demonstrating admirable properties to stabilize entrapped biomolecules. The microenvironment inside these materials, especially the distribution of sugar moieties inside the matrix, which is likely the most critical factor determining compatibility of these materials, still remains unclear. To deeply investigate the biocompatibility mechanism of these materials, we have adopted two different preparation routes for these materials by introducing GLS into the starting DGS sol stage, but things are different after the DGS gel is formed. A fluorescence probe rhodamine 6G is introduced herein in the DGS sol to monitor the distribution of GLS moieties, as well as the evolution of the microenvironment inside resulting materials. All in all, the findings demonstrated that the timing of GLS addition plays a critical role in controlling the evolution of the inner structure of materials, suggesting that this factor provides a promising route to tune the properties of the resulting materials.biocompatibility, carbon-silicon complex, microenvironment, characterization

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Microenvironment characterization for a novel biocompatible hybrid carbon-silicon material

Sui

Zha

et al. 2009

Sci. China Ser. B-Chem.

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“…Several additives can be added to the sol-gel in different phases of the preparation, resulting in "tailored materials" suitable for a number of proteins and detection systems [50]. Table 1 summarizes some applications of silica gel-encapsulated proteins reported in the literature [183,194,[200][201][202][203][204][205][206][207][208][209][210][211][212][213][214][215][216][217]. Notable examples include cases in which more proteins are co-encapsulated to immobilize short metabolic pathways [194].…”

Section: Proteins Encapsulated In Silica Gel As Biosensorsmentioning

confidence: 99%

Immobilization of Proteins in Silica Gel: Biochemical and Biophysical Properties

Ronda

Bruno

Campanini

et al. 2015

COC

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Abstract:The development of silica-based sol-gel techniques compatible with the retention of protein structure and function started more than 20 years ago, mainly for the design of biotechnological devices or biomedical applications. Silica gels are optically transparent, exhibit good mechanical stability, are manufactured with different geometries, and are easily separated from the reaction media. Biomolecules encapsulated in silica gel normally retain their structural and functional properties, are stabilized with respect to chemical and physical insults, and can sometimes exhibit enhanced activity in comparison to the soluble form. This review briefly describes the chemistry of protein encapsulation within the pores of a silica gel three-dimensional network, the mechanism of interaction between the protein and the gel matrix, and its effects on protein structure, function, stability and dynamics. The main applications in the field of biosensor design are described. Special emphasis is devoted to silica gel encapsulation as a tool to selectively stabilize subsets of protein conformations for biochemical and biophysical studies, an application where silica-based encapsulation demonstrated superior performance with respect to other immobilization techniques.

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“…A similar column was interfaced with MALDI MS/MS [67]. Other ligands that have been studied using sol-gel entrapment and capillary columns include nicotinic acetylcholine receptor from Torpedo californica, dopamine D2Short receptor [62,63,69], protein tyrosine kinase [64], firefly luciferase [65], aptamers [68], and antifluorescein antibodies [66].…”

Section: Studies Of Biological Interactionsmentioning

confidence: 99%

Affinity monolith chromatography

Mallik

Hage²

2006

J of Separation Science

190

282

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The combined use of monolithic supports with selective affinity ligands as stationary phases has recently given rise to a new method known as affinity monolith chromatography (AMC). This review will discuss the basic principles behind AMC and examine the types of supports and ligands that have been employed in this method. Approaches for placing affinity ligands in monoliths will be considered, including methods based on covalent immobilization, biospecific adsorption, entrapment, and the formation of coordination complexes. Several reported applications will then be presented, such as the use of AMC for bioaffinity chromatography, immunoaffinity chromatography, immobilized metal-ion affinity chromatography, dye-ligand affinity chromatography, and biomimetic chromatography. Other applications that will be discussed are chiral separations and studies of biological interactions based on AMC.

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Ultrasensitive ATP Detection Using Firefly Luciferase Entrapped in Sugar-Modified Sol−Gel-Derived Silica

Cited by 98 publications

References 24 publications

RETRACTED ARTICLE: Microenvironment characterization for a novel biocompatible hybrid carbon-silicon material

RETRACTED ARTICLE: Microenvironment characterization for a novel biocompatible hybrid carbon-silicon material

Immobilization of Proteins in Silica Gel: Biochemical and Biophysical Properties

Affinity monolith chromatography

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