Ultrasensitive detection of certain microbial metabolites by gas chromatography

Mitruka, B. M.; Alexander, Martin

doi:10.1016/0003-2697(67)90300-4

Cited by 15 publications

(7 citation statements)

References 4 publications

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“…We have previously demonstrated, with authentic chemicals, the extreme responsiveness of ECD to certain typical microbial metabolites (5). By applying techniques employing this remarkably sensitive detector to bacteria proliferating in a normal growth medium, it was possible to detect the activity associated with very smaU numbers of cells.…”

Section: Discussionmentioning

confidence: 99%

Rapid and Sensitive Detection of Bacteria by Gas Chromatography

Mitruka¹,

Alexander²

1968

Applied Microbiology

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A gas chromatograph fitted with electron capture and flame ionization detectors was employed for the rapid detection of bacteria by analysis for their metabolic products. The presence of Proteus vulgaris, Streptococcus faecalis, S. liquefaciens, Escherichia coli B, Bacillus cereus, and B. popilliae was detected in 2 to 4 hr in media inoculated with less than 104 cells per ml, whereas a 7to 12-hr growth period was required for the detection of products formed in cultures of Serratia marcescens, Aerobacter aerogenes, E. coli K-12, Staphylococcus aureus, and Salmonella typhimurium. Metabolites elaborated by the equivalent of less than a single cell of B. cereus, S. faecalis, P. vulgaris, or E. coli B were sensed by the electron capture detector. The flame ionization detector was generally not as sensitive. Volatile metabolites were identified, and their concentrations were determined.

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Section: Discussionmentioning

confidence: 99%

Rapid and Sensitive Detection of Bacteria by Gas Chromatography

Mitruka¹,

Alexander²

1968

Applied Microbiology

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“…Since the relevant metabolic changes occur on a very small scale [in the nano or picogram level (Mitruka and Alexander, 1967)] their detection by conventional chemical methods may be very difficult. Gas chromatographic (GC) techniques, on the other hand, are known to be very adequate for detecting quantities in this range.…”

Section: Introductionmentioning

confidence: 99%

“…in the cell membrane, leading to leakage of some intracellular components into the surrounding medium (Levanon, Klibansky, and Kohn, 1973;Sinyak el al., 1970). Since the relevant metabolic changes occur on a very small scale [in the nano or picogram level (Mitruka and Alexander, 1967)] their detection by conventional chemical methods may be very difficult. Gas chromatographic (GC) techniques, on the other hand, are known to be very adequate for detecting quantities in this range.…”

Section: Introductionmentioning

confidence: 99%

Picorna‐ and togavirus infection of cells detected by gas chromatography

Levanon

Klibansky

Kohn

1977

Journal of Medical Virology

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Viral infection of cells causes chemical and metabolic changes, which can be detected by gas chromatography (GC) of ether extracts of supernatant fluids and cell homogenates before any significant damage to the cells is observable microscopically. The characteristic and specific GC patterns obtained from BHK-21 and Vero cell cultures infected with encephalomyocarditis, polio, echoviruses, and a togavirus make it possible to distinguish between these infecting viruses. The appearance of 1 or 2 compounds, represented by GC peaks with TR values of 302 and 677 seconds seems to be specific for these viruses. Other peaks found in the supernatant media 1-2 hours after infection probably represent cell constituents leaking into the medium as a result of damage to the cell membrane by the invading virus.

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“…In previous studies, we have reported on the application of gas chromatographic techniques for the rapid and sensitive detection of viable bacteria and viruses (8,9). The electron capture detector, in particular, was found to be extremely sensitive for the detection of several microbial metabolites (7). Although several investigators have employed gas chromatographic methods for the analysis of chemical components in biological and clinical specimens (1,2,5,10,11), few studies have dealt with a systematic examination of pathogens with a view towards diagnostic application.…”

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Rapid Detection of Bacteremia in Mice by Gas Chromatography

1970

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Gas chromatography of the serum of mice inoculated 6 hr earlier with Staphylococcus aureus, Escherichia coli K-12, and Clostridium chauvoei revealed the formation of products associated with the infections. Longer periods were required before compounds resulting from infecting the animals with Salmonella typhimuriun and other Clostridium species were detected by gas chromatographic means. The technique is sensitive to the presence of metabolites elaborated in vivo as a result of the presence of 100 to 35,000 cells. Each bacterial species caused the formation of distinctive compounds in infected mice, and substances chromatographically identical to these products were generated by the microorganisms in vitro as well. Many species of bacteria may produce bacteremia in man and animals. Frequently, an exact and rapid identification of the offending micro

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Ultrasensitive detection of certain microbial metabolites by gas chromatography

Cited by 15 publications

References 4 publications

Rapid and Sensitive Detection of Bacteria by Gas Chromatography

Rapid and Sensitive Detection of Bacteria by Gas Chromatography

Picorna‐ and togavirus infection of cells detected by gas chromatography

Rapid Detection of Bacteremia in Mice by Gas Chromatography

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