Ultrasensitive detection of E. coli O157:H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor

Chan, Ka Yiu; Ye, Wei Wei; Zhang, Yu; Xiao, Li; Leung, Polly H.M.; Li, Yi; Yang, Mo

doi:10.1016/j.bios.2012.09.016

Cited by 115 publications

(42 citation statements)

References 26 publications

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“…In a novel approach, antibody-tagged biofunctional magnetic beads were used to facilitate the migration of target bacteria to the sensor surface, (92). The immunosensor was constructed on silanized, nonporous alumina, which was separated by two compartments with fluid accessibility.…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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SUMMARY Bacterial pathogens are important targets for detection and identification in medicine, food safety, public health, and security. Bacterial infection is a common cause of morbidity and mortality worldwide. In spite of the availability of antibiotics, these infections are often misdiagnosed or there is an unacceptable delay in diagnosis. Current methods of bacterial detection rely upon laboratory-based techniques such as cell culture, microscopic analysis, and biochemical assays. These procedures are time-consuming and costly and require specialist equipment and trained users. Portable stand-alone biosensors can facilitate rapid detection and diagnosis at the point of care. Biosensors will be particularly useful where a clear diagnosis informs treatment, in critical illness (e.g., meningitis) or to prevent further disease spread (e.g., in case of food-borne pathogens or sexually transmitted diseases). Detection of bacteria is also becoming increasingly important in antibioterrorism measures (e.g., anthrax detection). In this review, we discuss recent progress in the use of biosensors for the detection of whole bacterial cells for sensitive and earlier identification of bacteria without the need for sample processing. There is a particular focus on electrochemical biosensors, especially impedance-based systems, as these present key advantages in terms of ease of miniaturization, lack of reagents, sensitivity, and low cost.

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Section: Electrochemical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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“…Moreover with advances in micro-patterned paper devices 86 it seems increasing unlikely that the importance of field based paper diagnostics will significantly dim- inish in the near future. Improvements in nucleic acid and immunological testing via the employment of multi-functionality nanocomposites 59,60 , digitization of the pre-sampling 35 and the enrichment stages will undoubtedly led to an increase in the successful quantitative detection of viable Escherichia coli O157 : H7 and a reduction in the negative consequences of a future outbreak.…”

Section: Discussionmentioning

confidence: 99%

Recent trends in the detection of pathogenic Escherichia coli O157 : H7

Hulme

2015

BioChip J

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The rapid and accurate detection of pathogenic Escherichia coli (E. coli) poses a significant health problem in both developed and developing countries around the world. Conventional microbial detection methods can take more than 48 hours to identify a pathogenic organism. Recently new types of nucleic acid chip based methods, microfluidic devices, signal amplification methods, immunoassays and biosensors have been developed capable of detecting very low concentrations of pathogenic E. coli in a few hours. This review examines the current limitations and recent advances in methodologies employed in the rapid detection of pathogenic E. coli O157 : H7.

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“…Many nanoporous membrane based electrochemical sensors have been used in various applications including ion channel detection [19], DNA hybridization sensing [20,21], virus detection [22], cell based biosensing [23] and bacteria detection [24,25]. However, the current nanoporous membrane based methods were only used for single type of bacteria detection and could not realize the simultaneous detection of multiple types of bacteria from the mixed bacteria samples.…”

Section: Concurrent Detection Of Foodborne Pathogensmentioning

confidence: 99%

Concurrent Detection of Foodborne Pathogens: Past Efforts and Recent Trends

Evrendilek¹,

Richter²

2017

J Food Microbiol Saf Hyg

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Rapid and specific identification of foodborne pathogens is important for not only for food producers but also consumers as well as food safety authorities. Foods can harbor pathogen bacteria of different species, thus it is also important to be able to detect different pathogens simultaneously from the food samples even though they are in very low number. Detection of bacteria depends on different factors such as the initial number, food matrix, sensitivity of the method, competitor microflora and physical state of the bacteria whether they are injured or not. Simultaneous detection of different bacteria yet adds another hurdle for the level of detection, and thus, different approaches have developed and tested so far to optimize the maximum recovery.

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Ultrasensitive detection of E. coli O157:H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor

Cited by 115 publications

References 26 publications

Biosensors for Whole-Cell Bacterial Detection

Biosensors for Whole-Cell Bacterial Detection

Recent trends in the detection of pathogenic Escherichia coli O157 : H7

Concurrent Detection of Foodborne Pathogens: Past Efforts and Recent Trends

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