Ultrasensitive Detection of Ebola Virus Oligonucleotide Based on Upconversion Nanoprobe/Nanoporous Membrane System

Tsang, Ming Kiu; Ye, Weiwei; Wang, Guojing; Li, Jingming; Yang, Mo; Hao, Jianhua

doi:10.1021/acsnano.5b05622

Cited by 172 publications

(114 citation statements)

References 39 publications

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“…[14] Therefore, they were usually employed as quenchers in nanoparticle-based detection systems. [23][24][25][26][27][28] The assays involved highly distance-dependent luminescence resonance energy transfer (LRET) between energy donor and acceptor, in which the luminescent intensity changes with the separation between the energy pair. [18][19][20] The excitation may induce background fluorescence and broad emission peaks, contributing to severe cross-talking problem.…”

mentioning

confidence: 99%

“…[18][19][20] The excitation may induce background fluorescence and broad emission peaks, contributing to severe cross-talking problem. [23][24][25][26][27][28] The assays involved highly distance-dependent luminescence resonance energy transfer (LRET) between energy donor and acceptor, in which the luminescent intensity changes with the separation between the energy pair. They not only display large anti-Stokes shift [21,22] but also pose minimal damage to the gene oligos.…”

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confidence: 99%

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Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

Tsang

Wong

Tsoi

et al. 2019

Adv Healthcare Materials

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Those sensors increase the limits of detection (LOD) by using signal amplifiers but the hybridization times are usually long. [7] On the other hand, colorimetric sensor produce visible color changes upon the presence of the virus genes. The simplicity in design and readout of these sensors had attracted substantial attention. Gold nanoparticles (AuNPs) are one of the promising candidates for such detection strategy because of their high extinction coefficient and aggregation-dependent color change. [8,9] Despite the advantages, AuNPs are surface-sensitive and may induce undesired aggregations due to high ionic strength and concentration of ions, resulting in false-positive signals.Apart from those approaches, optical detection has drawn substantial attention because of their simplicity, sensitiveness, and multiplexity. [10,11] It is the fact that organic dyes are widely used in conventional qPCR assays via downconversion processes. [4,12,13] However, the dyes demonstrate undesirable emissive properties such as poor photostability, broad emission band, and small Stokes shift. [14] Therefore, they were usually employed as quenchers in nanoparticle-based detection systems. [15][16][17] High energy excited quantum dots (QDs) are another representative fluorophores in downconverting assays. [18][19][20] The excitation may induce background fluorescence and broad emission peaks, contributing to severe cross-talking problem. To address these limitations, nearinfrared (NIR) triggered upconversion luminescence (UCL) in lanthanide-doped upconversion nanoparticles (UCNPs) is very promising in biodetection. They not only display large anti-Stokes shift [21,22] but also pose minimal damage to the gene oligos. In addition, the long-lived UCL signal is easily distinguished from the short-lived background signal. As a result, UCNPs were used to detect different oligos and analytes. [23][24][25][26][27][28] The assays involved highly distance-dependent luminescence resonance energy transfer (LRET) between energy donor and acceptor, in which the luminescent intensity changes with the separation between the energy pair. [29] In this work, green emitting UCNPs and AuNPs are selected as the LRET pair owing to their well-matched emission and extinction spectrum, while AuNPs exhibit excellent extinction coefficient and photostability. The size of the nanoparticles played important roles in hybridization [30] because AuNPs demonstrate sizedependent absorption property and it can alter the stability of the hybridized DNA duplex. In addition, the size ratios of the UCNPs and AuNPs affect the formation of UCNPs-AuNPs hybrid structures. Taking advantages of 808 nm excitation of With large anti-Stokes shifts and background-free signals, upconversion luminescent (UCL) screening assays have been a promising method to reduce the transmission of influenza epidemic, which can critically alleviate the disease burden and extra annual deaths. In this work, a luminescent resonance energy transfer sandwich assay is developed, which utilizes core-shell...

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confidence: 99%

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confidence: 99%

Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

Tsang

Wong

Tsoi

et al. 2019

Adv Healthcare Materials

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“…RE 3+ ions possess vast amounts of energy levels, which provide convenience for us to realize optical modulation by adopting facile physical approaches26272829303132. Moreover, tunable excitation and emission wavelengths from visible to near-infrared (NIR) of RE 3+ -doped GCs guarantee optical modulation operating at telecom windows or at the visible range for emerging Li-Fi technology, showing notable advantages for optical data transmission systems133. Nevertheless, for a long time, fast-slow optical modulation of up-conversion (UC) fluorescence from RE 3+ ion single-doped GCs by utilizing a strategy named “two-step excitation of two-wavelengths” has been overlooked3435.…”

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confidence: 99%

Controllable optical modulation of blue/green up-conversion fluorescence from Tm3+ (Er3+) single-doped glass ceramics upon two-step excitation of two-wavelengths

Chen

Kang

Zhang

et al. 2017

Sci Rep

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Optical modulation is a crucial operation in photonics for network data processing with the aim to overcome information bottleneck in terms of speed, energy consumption, dispersion and cross-talking from conventional electronic interconnection approach. However, due to the weak interactions between photons, a facile physical approach is required to efficiently manipulate photon-photon interactions. Herein, we demonstrate that transparent glass ceramics containing LaF3: Tm3+ (Er3+) nanocrystals can enable fast-slow optical modulation of blue/green up-conversion fluorescence upon two-step excitation of two-wavelengths at telecom windows (0.8–1.8 μm). We show an optical modulation of more than 1500% (800%) of the green (blue) up-conversion fluorescence intensity, and fast response of 280 μs (367 μs) as well as slow response of 5.82 ms (618 μs) in the green (blue) up-conversion fluorescence signal, respectively. The success of manipulating laser at telecom windows for fast-slow optical modulation from rear-earth single-doped glass ceramics may find application in all-optical fiber telecommunication areas.

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“…Nanoporous alumina membranes have become a hot research area because of their established fabrication processes, nano-ordered structures, dramatically large surface areas, easy surface modifications, high surface reaction rate, as well as their intensified output signals [24]. Wide applications of nanoporous alumina membranes are in areas of DNA detection, lipid membrane-based biosensors, foodborne pathogen biosensing, and virus and cancer cell detection [25,26,27,28,29,30,31]. However, the application of nanoporous alumina membranes for histamine determination has not yet been explored.…”

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A Nanoporous Alumina Membrane Based Electrochemical Biosensor for Histamine Determination with Biofunctionalized Magnetic Nanoparticles Concentration and Signal Amplification

Zheng

et al. 2016

Sensors

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Histamine is an indicator of food quality and indispensable in the efficient functioning of various physiological systems. Rapid and sensitive determination of histamine is urgently needed in food analysis and clinical diagnostics. Traditional histamine detection methods require qualified personnel, need complex operation processes, and are time-consuming. In this study, a biofunctionalized nanoporous alumina membrane based electrochemical biosensor with magnetic nanoparticles (MNPs) concentration and signal amplification was developed for histamine determination. Nanoporous alumina membranes were modified by anti-histamine antibody and integrated into polydimethylsiloxane (PDMS) chambers. The specific antibody modified MNPs were used to concentrate histamine from samples and transferred to the antibody modified nanoporous membrane. The MNPs conjugated to histamine were captured in the nanopores via specific reaction between histamine and anti-histamine antibody, resulting in a blocking effect that was amplified by MNPs in the nanopores. The blockage signals could be measured by electrochemical impedance spectroscopy across the nanoporous alumina membrane. The sensing platform had great sensitivity and the limit of detection (LOD) reached as low as 3 nM. This biosensor could be successfully applied for histamine determination in saury that was stored in frozen conditions for different hours, presenting a potentially novel, sensitive, and specific sensing system for food quality assessment and safety support.

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Ultrasensitive Detection of Ebola Virus Oligonucleotide Based on Upconversion Nanoprobe/Nanoporous Membrane System

Cited by 172 publications

References 39 publications

Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

Controllable optical modulation of blue/green up-conversion fluorescence from Tm3+ (Er3+) single-doped glass ceramics upon two-step excitation of two-wavelengths

A Nanoporous Alumina Membrane Based Electrochemical Biosensor for Histamine Determination with Biofunctionalized Magnetic Nanoparticles Concentration and Signal Amplification

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