Ultrasensitive Detection of HIV-1 p24 Antigen Using Nanofunctionalized Surfaces in a Capacitive Immunosensor

Teeparuksapun, Kosin; Hedström, Martin; Wong, Eric; Tang, Shixing; Hewlett, Indira; Mattìasson, Bo

doi:10.1021/ac102144a

Cited by 79 publications

(72 citation statements)

References 25 publications

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“…Capacitive biosensors based on a potential pulse have been used in many applications [1,11,61,62] including microcontact imprinting as seen in Table 3. In this type of capacitive biosensors, a small potential pulse is applied to the working electrode and the capacitance is measured.…”

Section: Applications Of Microcontact Imprinting Methods With Capacmentioning

confidence: 99%

“…The same strategy was used to develop a capacitive biosensor for sensitive detection of HIV-1 p24 antigen [11]. Following polytyramine electro-polymerization on the gold electrode surface, gold nanoparticles were incorporated onto the electrode and then, anti-HIV-1 p24 monoclonal antibodies were immobilized on top.…”

Section: Different Applications Of Capacitive Biosensorsmentioning

confidence: 99%

“…The second factor is the increased surface area on the electrode via the use of immobilized AuNPs. The gold nanoparticles also significantly contributed to the decrease in the interfacial resistance which facilitated the electron transfer at the electrode surface [11]. …”

Section: Different Applications Of Capacitive Biosensorsmentioning

confidence: 99%

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Capacitive Biosensors and Molecularly Imprinted Electrodes

Ertürk

Mattìasson

2017

Sensors

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Abstract:Capacitive biosensors belong to the group of affinity biosensors that operate by registering direct binding between the sensor surface and the target molecule. This type of biosensors measures the changes in dielectric properties and/or thickness of the dielectric layer at the electrolyte/electrode interface. Capacitive biosensors have so far been successfully used for detection of proteins, nucleotides, heavy metals, saccharides, small organic molecules and microbial cells. In recent years, the microcontact imprinting method has been used to create very sensitive and selective biorecognition cavities on surfaces of capacitive electrodes. This chapter summarizes the principle and different applications of capacitive biosensors with an emphasis on microcontact imprinting method with its recent capacitive biosensor applications.

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Section: Applications Of Microcontact Imprinting Methods With Capacmentioning

confidence: 99%

Section: Different Applications Of Capacitive Biosensorsmentioning

confidence: 99%

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Capacitive Biosensors and Molecularly Imprinted Electrodes

Ertürk

Mattìasson

2017

Sensors

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“…Current HIV-1 diagnostics are largely based on the presence of anti-viral antibody in the blood [28], [29], [30]. Of particular concern is that elevated viral levels during acute infection contribute to as much as 26 times more efficient sexual transmission than that of chronic infection [3], [31].…”

Section: Introductionmentioning

confidence: 99%

The Use of Nanotrap Particles Technology in Capturing HIV-1 Virions and Viral Proteins from Infected Cells

et al. 2014

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HIV-1 infection results in a chronic but incurable illness since long-term HAART can keep the virus to an undetectable level. However, discontinuation of therapy rapidly increases viral burden. Moreover, patients under HAART frequently develop various metabolic disorders and HIV-associated neuronal disease. Today, the main challenge of HIV-1 research is the elimination of the residual virus in infected individuals. The current HIV-1 diagnostics are largely comprised of serological and nucleic acid based technologies. Our goal is to integrate the nanotrap technology into a standard research tool that will allow sensitive detection of HIV-1 infection. This study demonstrates that majority of HIV-1 virions in culture supernatants and Tat/Nef proteins spiked in culture medium can be captured by nanotrap particles. To determine the binding affinities of different baits, we incubated target molecules with nanotrap particles at room temperature. After short sequestration, materials were either eluted or remained attached to nanotrap particles prior to analysis. The unique affinity baits of nanotrap particles preferentially bound HIV-1 materials while excluded albumin. A high level capture of Tat or Tat peptide by NT082 and NT084 particles was measured by western blot (WB). Intracellular Nef protein was captured by NT080, while membrane-associated Nef was captured by NT086 and also detected by WB. Selective capture of HIV-1 particles by NT073 and NT086 was measured by reverse transcriptase assay, while capture of infectious HIV-1 by these nanoparticles was demonstrated by functional transactivation in TZM-bl cells. We also demonstrated specific capture of HIV-1 particles and exosomes-containing TAR-RNA in patients' serum by NT086 and NT082 particles, respectively, using specific qRT-PCR. Collectively, our data indicate that certain types of nanotrap particles selectively capture specific HIV-1 molecules, and we propose to use this technology as a platform to enhance HIV-1 detection by concentrating viral proteins and infectious virions from infected samples.

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“…Traditionally, carboxyl or amino-functionalized polymers were applied to immobilize antibodies for the development of immunosensors [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%