Ultrasensitive Detection of Interleukin 6 by Using Silicon Nanowire Field-Effect Transistors

Hu, Wenyuan; Wu, Yu-Ming; Vu, Cao-An; Chen, Wen-Yih

doi:10.3390/s23020625

Cited by 12 publications

(9 citation statements)

References 38 publications

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“…31Apt has also been validated for use in electrochemical and field-effect transistor sensors. [52][53][54][55] (GT)15 is known to stably disperse SWCNT but does not have demonstrated affinity for IL-6. 56 Previous aptamer-based SWCNT sensors have utilized aptamer-anchor sequences to enable ideal conformation of aptamer binding site and enhanced protein recognition.…”

Section: Resultsmentioning

confidence: 99%

An optical aptamer-based cytokine nanosensor detects macrophage activation by bacterial toxins

Ryan,

Rahman,

Williams

2024

Preprint

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Overactive or dysregulated cytokine expression is hallmark of many acute and chronic inflammatory diseases. This is true for acute or chronic infection, neurodegenerative diseases, autoimmune diseases, cardiovascular disease, cancer, and others. Cytokines such as interleukin-6 (IL-6) are known therapeutic targets and biomarkers for such inflammatory diseases. Platforms for cytokine detection are therefore desirable tools for both research and clinical applications. Single-walled carbon nanotubes (SWCNT) are versatile nanomaterials with near-infrared fluorescence that can serve as transducers for optical sensors. When functionalized with an analyte-specific recognition element, SWCNT emission may become sensitive and selective towards the desired target. SWCNT-aptamer sensors are easily assembled, inexpensive, and biocompatible. In this work, we introduced a nanosensor design based on SWCNT and a DNA aptamer specific to IL-6. We first evaluated several SWCNT-aptamer constructs based on this simple direct complexation method, wherein the aptamer both solubilizes the SWCNT and confers sensitivity to IL-6. The sensor limit of detection, 105 ng/mL, lies in the relevant range for pathological IL-6 levels. Upon investigation of sensor kinetics, we found rapid response within seconds of antigen addition which continued over the course of three hours. We found that this sensor construct is stable, and the aptamer is not displaced from the nanotube surface during IL-6 detection. Finally, we investigated the ability of this sensor construct to detect macrophage activation caused by bacterial lipopolysaccharides (LPS) in an in vitro model of disease, finding rapid and sensitive detection of macrophage-expressed IL-6. We are confident further development of this sensor will have novel implications for diagnosis of acute and chronic inflammatory diseases, in addition to contributing to the understanding of the role of cytokines in these diseases.

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Section: Resultsmentioning

confidence: 99%

An optical aptamer-based cytokine nanosensor detects macrophage activation by bacterial toxins

Ryan,

Rahman,

Williams

2024

Preprint

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“…The outbreak of the COVID-19 pandemic started in late December 2019 and then spread quickly worldwide, leading to a global public health emergency. Hu et al [ 84 ] proposed an SiNW-FET platform for the detection of IL-6, a disease marker of COVID-19. By using anti-IL-6 aptamers to detect IL-6, SiNW-FET can achieve effective detection concentrations as low as 2.1 pg/mL (100 fM).…”

Section: Application Of Sinw-fet Biosensorsmentioning

confidence: 99%

Application of Silicon Nanowire Field Effect Transistor (SiNW-FET) Biosensor with High Sensitivity

Li,

Chen

et al. 2023

Sensors

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As a new type of one-dimensional semiconductor nanometer material, silicon nanowires (SiNWs) possess good application prospects in the field of biomedical sensing. SiNWs have excellent electronic properties for improving the detection sensitivity of biosensors. The combination of SiNWs and field effect transistors (FETs) formed one special biosensor with high sensitivity and target selectivity in real-time and label-free. Recently, SiNW-FETs have received more attention in fields of biomedical detection. Here, we give a critical review of the progress of SiNW-FETs, in particular, about the reversible surface modification methods. Moreover, we summarized the applications of SiNW-FETs in DNA, protein, and microbial detection. We also discuss the related working principle and technical approaches. Our review provides an extensive discussion for studying the challenges in the future development of SiNW-FETs.

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“…Of these, immunosorbent assays offer the lowest limits of IL-6 detection in human plasma, as low as 0.5 pg/mL. , However, these techniques require long incubation periods, trained personnel, and proper instrumentation, making them incompatible for routine clinical diagnosis, frequent testing, and monitoring. , To mitigate these limitations, immunosensing antibody-based nanosensors have emerged as a user-friendly point-of-care alternative . For example, nanosensors for IL-6 based on silica nanowires and metal nanoparticles have been reported. − …”

Section: Introductionmentioning

confidence: 99%

“… 35 For example, nanosensors for IL-6 based on silica nanowires and metal nanoparticles have been reported. 37 − 40 …”

Section: Introductionmentioning

confidence: 99%

Optical Nanosensor Passivation Enables Highly Sensitive Detection of the Inflammatory Cytokine Interleukin-6

Gaikwad,

Rahman,

Parikh

et al. 2024

ACS Appl. Mater. Interfaces

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Interleukin-6 (IL-6) is known to play a critical role in the progression of inflammatory diseases such as cardiovascular disease, cancer, sepsis, viral infection, neurological disease, and autoimmune diseases. Emerging diagnostic and prognostic tools, such as optical nanosensors, experience challenges in translation to the clinic in part due to protein corona formation, dampening their selectivity and sensitivity. To address this problem, we explored the rational screening of several classes of biomolecules to be employed as agents in noncovalent surface passivation as a strategy to screen interference from nonspecific proteins. Findings from this screening were applied to the detection of IL-6 by a fluorescent-antibody-conjugated single-walled carbon nanotube (SWCNT)-based nanosensor. The IL-6 nanosensor exhibited highly sensitive and specific detection after passivation with a polymer, poly-l-lysine, as demonstrated by IL-6 detection in human serum within a clinically relevant range of 25 to 25,000 pg/mL, exhibiting a limit of detection over 3 orders of magnitude lower than prior antibody-conjugated SWCNT sensors. This work holds potential for the rapid and highly sensitive detection of IL-6 in clinical settings with future application to other cytokines or disease-specific biomarkers.

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Ultrasensitive Detection of Interleukin 6 by Using Silicon Nanowire Field-Effect Transistors

Cited by 12 publications

References 38 publications

An optical aptamer-based cytokine nanosensor detects macrophage activation by bacterial toxins

An optical aptamer-based cytokine nanosensor detects macrophage activation by bacterial toxins

Application of Silicon Nanowire Field Effect Transistor (SiNW-FET) Biosensor with High Sensitivity

Optical Nanosensor Passivation Enables Highly Sensitive Detection of the Inflammatory Cytokine Interleukin-6

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