Ultrasensitive determination of ascorbic acid by using cobalt oxyhydroxide nanosheets to enhance the chemiluminescence of the luminol–H₂O₂ system

Abstract

Ascorbic acid (AA) as an essential vitamin in the human body participates in various physiological reactions and plays a key role in many biochemical processes. Therefore, it is of vital importance to monitor and quantify AA in commercial tablets, beverages and food. In this work, a rapid and ultrasensitive chemiluminescence (CL) system for the detection of AA was developed, in which ultrathin cobalt oxyhydroxide (CoOOH) nanosheets were applied in the conventional luminol-H 2 O 2 CL system. The results showed that ultrathin CoOOH nanosheets as a catalyzer remarkably improved the CL intensity of the CoOOH-luminol-H 2 O 2 system, up to about 1400-fold. Under the optimized conditions, the CL inhibition efficiencies increase linearly with the concentrations of AA in the range of 1-500 pmol L À1, and the limit of detection was 39 fmol L À1. Moreover, the proposed CL system was successfully applied in the determination of AA in medicinal tablets with satisfactory results.

Quantum dots‐based chemiluminescence probes: an overview

“…CL probes are usually characterized by a fast response, high sensitivity, wide linear dynamic range, extreme sensitivity, relatively simplicity (exciting source‐free) and the use of inexpensive apparatus for analyses; these characteristics have accounted for their continuous growth in use. Over recent decades liquid‐phase CL probes, including acidic potassium permanganate, acridinium esters, dioxetanes, hypohalites, luminol, manganese(IV), and tris(2,2‐bipyridine)ruthenium(III) and related derivative complexes, have been therefore become an ever more important measuring techniques in various fields, including environmental, pharmaceutical, clinical, agricultural, and food analyses. In well established CL analytical methods, analytes usually act as catalysts, reagents, quenchers or even an enhancers in CL reactions.…”

Efficient Cascade Resonance Energy Transfer in Dynamic Nanoassembly for Intensive and Long-Lasting Multicolor Chemiluminescence

“…Tris stands for tris(hydroxymethyl)aminomethane, a commonly used buffer, which can coordinate to Co 2+ and stabilize it in the CL system. 35 As shown in Figure 2a, the CL of LUM is strongly enhanced in the presence of any of the three natural cyclodextrins: α-CD, β-CD, and γ-CD. With the same CD concentration (14 mM), the highest CL enhancement is achieved with β-CD (4.7-fold), followed by γ-CD (2.7-fold) and α-CD (2.3-fold).…”

“…To demonstrate the function of β-CD nanoassembly for enhancing the CL intensity and the cascade FRET efficiency, we use a simple LUM-Tris-Co 2+ CL system as the model system (LUM 2.2 mM, Co 2+ 0.04 mM, Tris 12.4 mM, H 2 O 2 50 mM, see Supporting Information for details). Tris stands for tris­(hydroxymethyl)­aminomethane, a commonly used buffer, which can coordinate to Co 2+ and stabilize it in the CL system . As shown in Figure a, the CL of LUM is strongly enhanced in the presence of any of the three natural cyclodextrins: α-CD, β-CD, and γ-CD.…”

In Situ Formed Catalytic Interface for Boosting Chemiluminescence

“…Thus the monitoring of AA was critical for the medical assays and diagnosis . Since AA was a versatile scavenger for ROS which was a vital intermediate in CL reaction, hence the detection of AA based on CL sensor was feasible in theory. − …”