Ultrasensitive determination of NE-100, a novel sigma ligand, in human plasma by liquid chromatography and electrospray ionization tandem mass spectrometry combined with a column-switching technique

Yamaguchi, Junichi; Watanabe, Yumiko; Ohmichi, Mari; Jingu, Shigeji; Ogawa, Naoyoshi; Kokatsu, Jun; Fukushima, Kiyomi; Goto, Junichi

doi:10.1016/s0378-4347(99)00183-8

Cited by 6 publications

(5 citation statements)

References 22 publications

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“…Furthermore, when a relatively large volume (i.e., >200 µL) of plasma is required, additional evaporation and subsequent reconstitution of sample tend to be time‐consuming. In recent years, high throughput and automated on‐line sample extraction approaches have provided attractive alternatives to improve efficiency and reduce the labor for sample processing 13–30. Given the current paradigm shift toward automated and robotics systems, we explored the utility of an on‐line turbulent flow chromatography unit coupled to mass spectrometry.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Crystal Structure of IM‐6, a New Open Framework Cobalt–Gallium Phosphate with Ten‐ and Twelve‐Membered Pore Openings

Josien

Simon‐Masseron

Gramlich³

et al. 2003

Chemistry A European J

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A new three-dimensional microporous cobalt-gallium phosphate, named IM-6, has been synthesized under solvothermal conditions with an N-substituted piperazine as organic template. The structure was solved by single-crystal X-ray diffraction (triclinic, P(-)1, a=9.848(20), b=12.470(32), c=12.603(28)A, alpha=63.47(16) degrees, beta=74.56(16) degrees, gamma=76.03(17) degrees). IM-6 exhibits a new framework topology. The inorganic framework is built up of MO(4) (M=Co, Ga) and PO(4) tetrahedra. It displays a two-dimensional interconnected channel system running along the [0(-)11] and [100] directions and delimited by ten- and twelve-membered ring openings, respectively.

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Section: Resultsmentioning

confidence: 99%

Synthesis and Crystal Structure of IM‐6, a New Open Framework Cobalt–Gallium Phosphate with Ten‐ and Twelve‐Membered Pore Openings

Josien

Simon‐Masseron

Gramlich³

et al. 2003

Chemistry A European J

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“…Inadvertently, this has also created a window of opportunity to make greater use of stable isotope-labeled compounds for early metabolism studies. One of the consequences of the extensive use of LC/MS for quantitative assays has been an increase in the syntheses of stable isotope-labeled analogues that are often used as internal standards in these studies − . With the availability of these stable isotope-labeled analogues and robust LC/MS instruments, it has become straightforward to quantitate analytes in complex biological mixtures.…”

Section: Introductionmentioning

confidence: 99%

“…The focus of this review precludes us from discussing the value of having stable isotope-labeled analytes for quantitation purposes. The readers are directed to a number of publications focused on the application of LC/MS and stable isotope-labeled compounds for quantitative analyses from preclinical and clinical pharmacokinetic studies (77,(80)(81)(82)(83)(84)(85)(86)(87)(88)(89)(90)(91)(92)(93)(94)(95)(96)(97)(98). Other quantitative applications of stable isotope-labeled compounds include studies conducted to distinguish in vivo and in vitro disposition of enantiomers where only one of the enantiomers was selectively labeled with stable isotopes (5).…”

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confidence: 99%

Application of Stable Isotope-Labeled Compounds in Metabolism and in Metabolism-Mediated Toxicity Studies

Mutlib¹

2008

Chem. Res. Toxicol.

194

127

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Stable isotope-labeled compounds have been synthesized and utilized by scientists from various areas of biomedical research during the last several decades. Compounds labeled with stable isotopes, such as deuterium and carbon-13, have been used effectively by drug metabolism scientists and toxicologists to gain better understanding of drugs' disposition and their potential role in target organ toxicities. The combination of stable isotope-labeling techniques with mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy, which allows rapid acquisition and interpretation of data, has promoted greater use of these stable isotope-labeled compounds in absorption, distribution, metabolism, and excretion (ADME) studies. Examples of the use of stable isotope-labeled compounds in elucidating structures of metabolites and delineating complex metabolic pathways are presented in this review. The application of labeled compounds in mechanistic toxicity studies will be discussed by providing an example of how strategic placement of a deuterium atom in a drug molecule mitigated specific-specific renal toxicity. Other examples from the literature demonstrating the application of stable isotope-labeled compounds in understanding metabolism-mediated toxicities are presented. Furthermore, an example of how a stable isotope-labeled compound was utilized to better understand some of the gene changes in toxicogenomic studies is discussed. The interpretation of large sets of data produced from toxicogenomics studies can be a challenge. One approach that could be used to simplify interpretation of the data, especially from studies designed to link gene changes with the formation of reactive metabolites thought to be responsible for toxicities, is through the use of stable isotope-labeled compounds. This is a relatively unexplored territory and needs to be further investigated. The employment of analytical techniques, especially mass spectrometry and NMR, used in conjunction with stable isotope-labeled compounds to establish and understand mechanistic link between reactive metabolite formation, genomic, and proteomic changes and onset of toxicity is proposed. The use of stable isotope-labeled compounds in early human ADME studies as a way of identifying and possibly quantifying all drug-related components present in systemic circulation is suggested.

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“…The main applicability of this technique relies on plasma and serum analysis, finding less benefit in the pesticide exposure field where urine samples are the most practical and interesting ones. However, the coupled‐column approach (LC/LC) seems to be a more suitable technique for the direct injection and online sample clean‐up of urine (or serum) samples 17–21. In this technique, urine (or serum) samples are directly injected into a first separation column and only the fraction containing the analyte (chlorpyrifos or TCP) is transferred to the second column, which preferably will have a different retention mechanism.…”

Section: Introductionmentioning

confidence: 99%

Direct determination of chlorpyrifos and its main metabolite 3,5,6-trichloro-2-pyridinol in human serum and urine by coupled-column liquid chromatography/electrospray-tandem mass spectrometry

Sancho

Pozo

Hernández

2000

Rapid Commun. Mass Spectrom.

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Ultrasensitive determination of NE-100, a novel sigma ligand, in human plasma by liquid chromatography and electrospray ionization tandem mass spectrometry combined with a column-switching technique

Cited by 6 publications

References 22 publications

Synthesis and Crystal Structure of IM‐6, a New Open Framework Cobalt–Gallium Phosphate with Ten‐ and Twelve‐Membered Pore Openings

Synthesis and Crystal Structure of IM‐6, a New Open Framework Cobalt–Gallium Phosphate with Ten‐ and Twelve‐Membered Pore Openings

Application of Stable Isotope-Labeled Compounds in Metabolism and in Metabolism-Mediated Toxicity Studies

Direct determination of chlorpyrifos and its main metabolite 3,5,6-trichloro-2-pyridinol in human serum and urine by coupled-column liquid chromatography/electrospray-tandem mass spectrometry

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