Ultrasensitive DNA Detection Using Oligonucleotide−Silver Nanoparticle Conjugates

Thompson, D G; Enright, Alexis; Faulds, Karen; Smith, W. Ewen; Graham, Duncan

doi:10.1021/ac702403w

Cited by 242 publications

(190 citation statements)

References 28 publications

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“…The same strategy of ligand exchange has also been applied to Au rods (Dujardin et al 2001), silver nanoparticles (Thompson et al 2008), MPAstabilized CdSe/ZnS quantum dots (Mitchell et al 1999;Gill et al 2005) and Pt nanoparticles decorated with amino-modified DNA (Yang et al 2004).…”

Section: (I) Biotin Avidin and Derivativesmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,409

1,092

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Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the form of fluorescence (semiconductor quantum dots, e.g. CdSe or CdTe) or phosphorescence (doped oxide materials, e.g. Y 2 O 3 ), or magnetic moment (e.g. iron oxide or cobalt nanoparticles). Prerequisite for every possible application is the proper surface functionalization of such nanoparticles, which determines their interaction with the environment. These interactions ultimately affect the colloidal stability of the particles, and may yield to a controlled assembly or to the delivery of nanoparticles to a target, e.g. by appropriate functional molecules on the particle surface. This work aims to review different strategies of surface modification and functionalization of inorganic colloidal nanoparticles with a special focus on the material systems gold and semiconductor nanoparticles, such as CdSe/ZnS. However, the discussed strategies are often of general nature and apply in the same way to nanoparticles of other materials.

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Section: (I) Biotin Avidin and Derivativesmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,409

1,092

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“…This method of synthesis is clean, less costly, employs ambient conditions and less energy intensive . The diverse application of nanocrystalline silver ranges from catalysis (Jana et al, 1999) to photonics (Velikov et al, 2003), biosensing and diagnostics (Songping andShuyuan, 2005, Schultz et al, 2000), antimicrobial (Pal et al, 2007) DNA sequencing (Thompson et al, 2008). In one study, antimicrobial activity of silver nanoparticles was shown to promote wound healing (Ghosh et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antibacterial effect

Kumar¹,

Darshit²,

Ankita³

et al. 2011

Afr. J. Biotechnol.

107

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A green synthesis of silver nanoparticle was carried out using tobacco leaf extract. Synthesized nanoparticles were characterized using UV-Vis absorption spectroscopy, TEM, EDAX, FT-IR and photoluminescence study, respectively. UV-Vis absorption spectroscopy of prepared silver colloidal solution showed absorption maxima at 418 nm. Excitation maximum and emission maximum obtained from photoluminescence study were found at 414 and 576 nm, respectively. TEM analysis showed average particle size of 8 nm, while SAED pattern confirmed the crystalline nature of synthesized nanoparticles. FT-IR analysis indicated the involvement of carboxyl (-C= O), hydroxyl (-OH) and amine (-NH) functional groups of tobacco leaf extract in preparation of silver nanoparticles. EDAX analysis showed proportion of silver (54.55%) among other elements in nanoparticle. Pseudomonas aeruginosa and Escherichia coli DH5α showed highest sensitivity towards silver nanoparticles.

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“…The most popular results based on such a nanoparticle assembly-based scheme have been comprehensively studied for the detection of DNA targets. For example, nanoparticle assemblies can be induced by combining two different types of DNAmodified silver nanoparticle (DNA-AgNP) probes with the target DNA sequence [36]. The two probe DNA sequences on the DNA-AgNPs are not complementary to the other, and therefore, the two types of DNA-AgNPs do not interact with each other and do stay dispersed in the absence of the target DNA.…”

Section: Lspr-based Detection Using the Assembly Formation Of Silver mentioning

confidence: 99%

“…The LSPR-based detection methods using assembly of spherical silver nanoparticles, or silver nanospheres (AgNSs), were first reported in 2008 by Graham et al [36]. Prior to their work, the AgNSs were demonstrated to be stably modified with probe DNA strands containing triple cyclic disulfide moieties by Mirkin et al [39,40].…”

Section: Silver Nanospheres (Agnss)mentioning

confidence: 99%

Silver nanomaterials for the detection of chemical and biological targets

Lee

2014

Nanotechnology Reviews

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Abstract:Silver nanomaterials have attracted a lot of interest from researchers, mainly owing to their distinctive optical properties such as localized surface plasmon resonance (LSPR) and surface-enhanced Raman scattering (SERS). These properties are particularly interesting for the diagnostic applications when combined with target-recognition capabilities of surface ligands and target concentration-dependent quantitative aspects of the LSPR or SERS signal. This review covers these two main optical properties of silver nanomaterials with respect to their sensing applications for various chemical and biological targets. The LSPR-based colorimetric detection schemes are further divided into two categories based on what they depend on: (1) assembly and (2) deformation of the silver nanoparticle probes. Because the various shapes of the silver nanomaterials are highly associated with how to design and control the LSPR-and SERS-based detection schemes, the latest progress in the development of highly sensitive and selective detection strategies are discussed with respect to the morphological diversity of the silver nanomaterials.

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Ultrasensitive DNA Detection Using Oligonucleotide−Silver Nanoparticle Conjugates

Cited by 242 publications

References 28 publications

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antibacterial effect

Silver nanomaterials for the detection of chemical and biological targets

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