Ultrasensitive Electrochemical Detection of Clostridium perfringens DNA Based Morphology-Dependent DNA Adsorption Properties of CeO2 Nanorods in Dairy Products

Abstract

Foodborne pathogens such as Clostridium perfringens can cause diverse illnesses and seriously threaten to human health, yet far less attention has been given to detecting these pathogenic bacteria. Herein, two morphologies of nanoceria were synthesized via adjusting the concentration of NaOH, and CeO2 nanorod has been utilized as sensing material to achieve sensitive and selective detection of C. perfringens DNA sequence due to its strong adsorption ability towards DNA compared to nanoparticle. The DNA probe was tightly immobilized on CeO2/chitosan modified electrode surface via metal coordination, and the DNA surface density was 2.51 × 10−10 mol/cm2. Under optimal experimental conditions, the electrochemical impedance biosensor displays favorable selectivity toward target DNA in comparison with base-mismatched and non-complementary DNA. The dynamic linear range of the proposed biosensor for detecting oligonucleotide sequence of Clostridium perfringens was from 1.0 × 10−14 to 1.0 × 10−7 mol/L. The detection limit was 7.06 × 10−15 mol/L. In comparison, differential pulse voltammetry (DPV) method quantified the target DNA with a detection limit of 1.95 × 10−15 mol/L. Moreover, the DNA biosensor could detect C. perfringens extracted DNA in dairy products and provided a potential application in food quality control.

Multiwalled Carbon Nanotubes-CeO2 Nanorods: A “Nanonetwork” Modified Electrode for Detecting Trace Rifampicin

“…CeO 2 nanorods were synthesized as reported in previous work [24]. Briefly, 2.0 g CeCl 3 •7H 2 O was dissolved in 10 mL water (solution a), 3.2 g NaOH was dissolved in 25 mL water (solution b), then both were mixed and stirred for 10 min.…”

Electrochemical Biosensors for Detection of Foodborne Pathogens

“…The electrochemical signal of DPV could be detected by “sandwich” reaction. Recently, Wang et al [87] described an electrochemical biosensor for the detection of DNA of C. perfringens based on CeO 2 /chitosan-modified electrodes by monitoring the changes of impedance.…”

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

“…Under optimal experimental conditions, electrochemical impedance measurements were selective toward target DNA in comparison with base-mismatched and noncomplementary DNA. The dynamic range for detecting the target oligonucleotide sequence of C. perfringens was 10 −14 -10 −7 mol•L −1 [120].…”