Ultrasensitive electrochemical detection of UO<sub>2</sub><sup>2+</sup> based on DNAzyme and isothermal enzyme-free amplification

Yun, Wen; Jiang, Jiali; Cai, Dingzhou; Wang, Xiaofang; Ge, Shenguang; Liao, Junsheng; Lu, Tiecheng; Yan, Kangping

doi:10.1039/c5ra22773a

Cited by 46 publications

(20 citation statements)

References 43 publications

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“…Different amplification strategies, such as hybridization chain reaction (HCR) and catalyzed hairpin assembly (CHA), were used for electrochemical biosensor development. Yun et al reported an ultrasensitive DNAzyme-based electrochemical sensor for uranyl detection ( Yun et al, 2016b ). DNAzyme was selectively cleaved in the presence of UO 2 2+ .…”

Section: Applications Of Different Types Of Dnazyme-based Sensorsmentioning

confidence: 99%

Recent Advances on DNAzyme-Based Biosensors for Detection of Uranyl

Bai

Chai

et al. 2022

Front. Chem.

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Nuclear facilities are widely used in fields such as national defense, industry, scientific research, and medicine, which play a huge role in military and civilian use. However, in the process of widespread application of nuclear technology, uranium and its compounds with high carcinogenic and biologically toxic cause a lot of environmental problems, such as pollutions of water, atmosphere, soil, or ecosystem. Bioensors with sensitivity and specificity for the detection of uranium are highly demand. Nucleic acid enzymes (DNAzyme) with merits of high sensitivity and selectivity for targets as excellent molecular recognition elements are commonly used for uranium sensor development. In this perspective review, we summarize DNAzyme-based biosensors for the quantitative detection of uranyl ions by integrating with diverse signal outputting strategies, such as fluorescent, colorimetry, surface-enhanced Raman scattering, and electrochemistry. Different design methods, limit of detection, and practical applications are fully discussed. Finally, the challenges, potential solutions, and future prospects of such DNAzyme-based sensors are also presented.

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Section: Applications Of Different Types Of Dnazyme-based Sensorsmentioning

confidence: 99%

Recent Advances on DNAzyme-Based Biosensors for Detection of Uranyl

Bai

Chai

et al. 2022

Front. Chem.

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“…The initiator opens H2, which subsequently opens H1 to form a nicked dsDNA polymer chain, which is again filled with minor groove binder MB to give an electrochemical signal, with an LOD of 20 pM (Fig. 3B) [64]. It should be noted that a further study uses the formation of a hairpin polymer for the electrochemical detection of Pb 2+ by tethering the enzyme sequence of a DNAzyme to a magnetic bead, and using the cleavage of the substrate and exposure of the enzyme sequence to initiate a hairpin chain assembly on the magnetic beads with a ferrocene-tagged hairpin, generating an electrochemical signal, with an LOD of 37 pM [67].…”

Section: Electrochemical Sensorsmentioning

confidence: 99%

DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells

McGhee

Loh

2017

Current Opinion in Biotechnology

128

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The on-site and real-time detection of metal ions is important for environmental monitoring and for understanding the impact of metal ions on human health. However, developing sensors selective for a wide range of metal ions that can work in the complex matrices of untreated samples and cells presents significant challenges. To meet these challenges, DNAzymes, an emerging class of metal ion-dependent enzymes selective for almost any metal ion, have been functionalized with fluorophores, nanoparticles and other imaging agents and incorporated into sensors for the detection of metal ions in environmental samples and for imaging the metal ions in living cells. Herein, we highlight the recent developments of DNAzyme-based fluorescent, colorimetric, SERS, electrochemical and electrochemiluminscent sensors for metal ions for these applications.

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“…Many analytical methods have been developed for UO 2 2+ detection such as electrochemical methods, 4 fluorescence spectroscopy, 5 UV–vis adsorption spectrometry, 6 ICP mass spectrometry (ICP-MS), 7 inductively coupled plasma atomic emission spectroscopy (ICP-AES), 8 and so on. Despite providing concentration information, those methods lack the capability of enforcing in situ analysis or require time-consuming sample preparation, sophisticated equipment, expert operators, and high cost, limiting their practical applications.…”

Section: Introductionmentioning

confidence: 99%

In Situ Surface-Enhanced Raman Spectroscopy Detection of Uranyl Ions with Silver Nanorod-Decorated Tape

et al. 2019

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Surface-enhanced Raman spectroscopy (SERS) has been utilized for rapid analysis of uranyl ions (UO 2 2+ ) on account of its fast response and high sensitivity. However, the difficulty of fabricating a suitable SERS substrate for in situ analysis of uranyl ions severely restricts its practical application. Hence, we proposed flexible and adhesive SERS tape decorated with silver nanorod (AgNR) arrays for in situ detection of UO 2 2+ . The SERS tape was fabricated through a simple “paste & peel off” procedure by transferring the slanted AgNR arrays from silicon to the transparent tape surface. UO 2 2+ can be easily in situ detected by placing the AgNR SERS tape into an aqueous solution or pasting it onto the solid matrix surface due to the excellent transparent feature of the tape. The proposed SERS tape with well-distributed AgNRs effectively improved the reproducibility and sensitivity for UO 2 2+ analysis. UO 2 2+ with concentration as low as 100 nM was easily detected. Besides, UO 2 2+ adsorbed on an iron disc and rock surface also can be rapidly in situ detected. With its simplicity and convenience, the AgNR SERS tape-based SERS technique offers a promising approach for environmental monitoring and nuclear accident emergency detection.

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Ultrasensitive electrochemical detection of UO₂²⁺ based on DNAzyme and isothermal enzyme-free amplification

Abstract: A novel enzyme-free amplification biosensor for uranyl detection was developed based on UO22+-specific DNAzyme and a hybridization chain reaction.

Cited by 46 publications

References 43 publications

Recent Advances on DNAzyme-Based Biosensors for Detection of Uranyl

Recent Advances on DNAzyme-Based Biosensors for Detection of Uranyl

DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells

In Situ Surface-Enhanced Raman Spectroscopy Detection of Uranyl Ions with Silver Nanorod-Decorated Tape

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Ultrasensitive electrochemical detection of UO22+ based on DNAzyme and isothermal enzyme-free amplification

Abstract: A novel enzyme-free amplification biosensor for uranyl detection was developed based on UO22+-specific DNAzyme and a hybridization chain reaction.

Cited by 46 publications

References 43 publications

Recent Advances on DNAzyme-Based Biosensors for Detection of Uranyl

Recent Advances on DNAzyme-Based Biosensors for Detection of Uranyl

DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells

In Situ Surface-Enhanced Raman Spectroscopy Detection of Uranyl Ions with Silver Nanorod-Decorated Tape

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Ultrasensitive electrochemical detection of UO₂²⁺ based on DNAzyme and isothermal enzyme-free amplification