Ultrasensitive Electrochemical DNA Biosensors Based on the Detection of a Highly Characteristic Solid‐State Process

Zhang, Jie; Ting, Boon Ping; Jana, Nikhil R.; Gao, Zhiqiang; Ying, Jackie Y.

doi:10.1002/smll.200900073

Cited by 81 publications

(74 citation statements)

References 22 publications

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“…Third, the detection of DNA using this approach is sensitive. We achieve, for example, low picomolar sensitivity for DNA detection, comparing very favorably with previously reported methods (Table 1) (28,35,(50)(51)(52). Fourth, each step in the process (pretreatment with Exonuclease, the binding of the target to the DNA probe, the addition of conjugated polyelectrolytes) is performed separately, allowing each step to be optimized independently.…”

Section: Discussionmentioning

confidence: 62%

Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes

Xia

Zuo

Yang

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

523

348

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We have demonstrated a novel sensing strategy employing single-stranded probe DNA, unmodified gold nanoparticles, and a positively charged, water-soluble conjugated polyelectrolyte to detect a broad range of targets including nucleic acid (DNA) sequences, proteins, small molecules, and inorganic ions. This nearly "universal" biosensor approach is based on the observation that, while the conjugated polyelectrolyte specifically inhibits the ability of single-stranded DNA to prevent the aggregation of gold-nanoparticles, no such inhibition is observed with double-stranded or otherwise "folded" DNA structures. Colorimetric assays employing this mechanism for the detection of hybridization are sensitive and convenient-picomolar concentrations of target DNA are readily detected with the naked eye, and the sensor works even when challenged with complex sample matrices such as blood serum. Likewise, by employing the binding-induced folding or association of aptamers we have generalized the approach to the specific and convenient detection of proteins, small molecules, and inorganic ions. Finally, this new biosensor approach is quite straightforward and can be completed in minutes without significant equipment or training overhead.biosensor | aptamer | visual detection | thrombin detection | cocaine detection G old nanoparticle colorimetric biosensors have seen significant applications in diagnostics, environmental monitoring, and antibioterrorism supporting unaided, visual readout (1-12). Commonly, the relevant nanoparticles are covalently modified with either a probe DNA or an aptamer such that hybridization (13-16) or aptamer-target interactions (17-27), for example the scanometric method developed by Mirkin (25), which is a very sensitive and specific tool, crosslink them, inducing aggregation. The second broad approach utilizes unmodified nanoparticles. (28-30) These two approaches, however, suffer from timeconsuming (20-40 h of assembly) and relatively poor (low nanomolar) detection limits, respectively. Here, a unique, colorimetric sensing strategy employing a simple but selective combination of a single-stranded DNA probe, a positively charged, water-soluble conjugated polyelectrolyte, and unmodified gold nanoparticles is demonstrated. The universality of this method allows detection of a broad range of targets, including nucleic acid (DNA) sequences, proteins, small molecules, and ino rganic ions. Our approach is rapid (turnaround time is 5-10 min) and sensitive (picomolar concentrations of target DNA are readily detected with the naked eye, even in complex sample matrices like blood serum). Hence, an operator with minimum scientific overhead can easily employ this technique.Generally, the gold nanoparticle applications typically rely on a quantitative coupling between target recognition and the aggregation of the nanoparticles, which, in turn, leads to a dramatic change in the photonic properties-and thus the color-of the nanoparticle solution. This colorimetric "readout" avoids the relative complexity inherent...

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Section: Discussionmentioning

confidence: 62%

Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes

Xia

Zuo

Yang

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

523

348

View full text Add to dashboard Cite

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“…In this progress, AgNPs was oxidized to Ag + , and the generated Ag + formed insoluble AgCl on the electrode surface when Cl − was existed in solution. Compared with stripping voltammetry, it can lead to a sharper peak at a lower potential, which is far from unwanted oxidative interferences [5][6][7][8]. Besides, it is worth mentioning that the current response of this voltammetry remains unchanged after scanning for 20 cycles.…”

Section: Electrochemical Characteristics Of the Agnpsmentioning

confidence: 99%

“…Especially, the introducing of solid-state voltammetry further promotes the development of biosensor involving AgNPs because this solid-state voltammetry could produce a sharper oxidation peak with intense peak current and flat baseline as compared to the other voltammetry [5]. Since Ying's group first used this type of voltammetry to detect prostate-specific antigen and DNA oligonucleotide of the avian flu virus H5N1 [6]. This voltammetry has been adopted for ultrasensitive determination of tumor markers [5], Bacillus thuringiensis transgenic sequence [7] and Escherichia coli [8].…”

Section: Introductionmentioning

confidence: 99%

Controlled Synthesis of Silver Nanoparticles Using Double Reductants and Its Voltammetric Characteristics Study

Duan

Jiang

2016

Bull. Chem. React. Eng. Catal.

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Constructing robust silver nanoparticles (AgNPs) with good shape and dispersibility is of particular interest in analytical applications. Herein, monodispersibility AgNPs with the average size of 20 nm have been successfully prepared via one-pot method using sodium borohydride and trisodium citrate as co-reductants. The introduction of sodium borohydride greatly accelerated the rate of nucleation, which can effectively solve the problem of broad size distribution. Both shape and dispersibility of AgNPs can be effectively adjusted by simple control of refluxing time or concentrations of the sodium borohydride. We also studied the voltammetric characteristics of the AgNPs using Ag/AgCl solid-state voltammetry. An intense and stable current peak at a low potential could be obtained, which could provide a unique advantage in analytical applications.

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“…Among which, metal nanoparticles such as silver or gold nanoparticles (AgNPs or AuNPs) have been extensively used in the preparation of biosensors to transduce and amplify the signal due to their unique and size-dependent optical and electronic properties, and such nanoparticles that mainly used were of negatively charged AgNPs or AuNPs. Recently, a variety of new intriguing techniques were developed based on the formation of DNA composites by using positively charged AuNPs or AgNPs adsorbed onto the negative charged surface of double-stranded DNA (dsDNA) [17][18][19][20][21][22]. In addition, numerous amplification protocols such as nuclease-based target-recycling, rolling circle amplification (RCA), polymerase chain reaction (PCR) and hybridization chain reaction (HCR) have also been utilized for the development of biosensors.…”

Section: Introductionmentioning

confidence: 99%

Hybridization chain reaction and gold nanoparticles dual signal amplification for sensitive glucose detection

Miao

Zhu

et al. 2015

Biochemical Engineering Journal

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Ultrasensitive Electrochemical DNA Biosensors Based on the Detection of a Highly Characteristic Solid‐State Process

Cited by 81 publications

References 22 publications

Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes

Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes

Controlled Synthesis of Silver Nanoparticles Using Double Reductants and Its Voltammetric Characteristics Study

Hybridization chain reaction and gold nanoparticles dual signal amplification for sensitive glucose detection

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