Ultrasensitive electrochemical sensing platform for miRNA-21 detection based on manganese dioxide-gold nanoparticle nanoconjugates coupled with hybridization chain reaction and horseradish peroxidase signal amplification

Abstract

Here, a ultrasensitive electrochemical miRNA-21 biosensor is described. In this study, manganese dioxide-gold nanoparticles (MnO2-Au NPs) nanoconjugates were employed as sensing base materials, miRNA-21 was selected as a model analyte,...

Ratiometric electrochemical biosensor based on hybridization chain reaction signal amplification for sensitive microRNA-155 detection

“…Thus, interference from surroundings is avoided, and results obtained are more accurate and convincing. Previously, we developed electrochemical biosensors for the detection of miRNA-21 and miRNA-222 based on nanomaterials and HCR signal amplification, 20,21 and these biosensors all used a single signal as the output signal to detect analytes. Here, we have designed a ratiometric electrochemical biosensor for miRNA-155 detection based on the electrochemical signal of Fc-labeled capture DNA as an internal signal, and the electrochemical signal of MB-labeled probe DNA as an indicator signal.…”