Ultrasensitive fluorescence detection of antitumor drug methotrexate based on a terbium-doped silica dendritic probe

Mohammadzadeh, Arezoo; Jouyban, Abolghasem; Hasanzadeh, Mohammad; Shafiei‐Irannejad, Vahid; Soleymani, Jafar

doi:10.1039/d1ay01098k

Cited by 10 publications

(4 citation statements)

References 48 publications

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“…Most of them suffer from the high limits of detection (Table S2). , The high detection limit blocks their real-world application to detect the trace amount of this pharmaceutical waste. At the same time, the other probes of methotrexate possess a long detection time, , which suppresses their real-time monitoring application.…”

Section: Introductionmentioning

confidence: 99%

Functional Group-Assisted Fluorescence Sensing Platform for Nanomolar-Level Detection of an Antineoplastic Drug and a Neurotransmitter from Environmental Water and Human Biofluids

Rana,

Mishra,

Biswas

2024

Inorg. Chem.

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A fast, sensitive, selective, and biocompatible dual sensor of an antineoplastic medication (methotrexate) and a neurotransmitter (adrenaline) is still being searched by present-day scientists. To overcome this issue, we have designed a functionalized, robust, bio-friendly luminescent MOF for the sensitive, selective, and rapid monitoring of methotrexate and adrenaline. This probe is the first ever reported MOF-based fluorescence sensor of methotrexate and second only for adrenaline. This fluorescence probe has a very low limit of detection (LOD) of 0.34 and 11.2 nM for adrenaline and methotrexate, respectively. The sensor can detect both the targeted analytes rapidly within 5 s. It can also detect adrenaline and methotrexate from human blood serum and urine accurately and precisely. This reusable sensor is equally efficient in detecting methotrexate from environmental water specimens. Biocompatible, user-friendly, and inexpensive chitosan@MOF@cotton composites were fabricated for the detection of adrenaline and methotrexate from the nanomolar to the micromolar range by the naked eye under a fluorescence lamp. This probe displayed high reproducibility, precision, and accuracy in sensing methotrexate and adrenaline. Fluorescence resonance energy transfer (FRET) and the inner filter effect (IFE) are the possible mechanisms for adrenaline and methotrexate sensing, respectively. The possible mechanism was supported by using required instrumental techniques and theoretical simulations.

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Section: Introductionmentioning

confidence: 99%

Functional Group-Assisted Fluorescence Sensing Platform for Nanomolar-Level Detection of an Antineoplastic Drug and a Neurotransmitter from Environmental Water and Human Biofluids

Rana,

Mishra,

Biswas

2024

Inorg. Chem.

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“…The method was found to be sensitive, but the procedure requires additional steps to prepare terbium-doped dendritic silica particles. 11 In a similar line, Goksel et al also developed a method for the detection of MTX in samples at clinically applicable levels engaging electro-chemical Surface-enhanced Raman spectroscopy on a customized benchtop Raman spectrometer. Such methods were also found to be valid for detecting MTX at the point of need but require a specialized Raman device.…”

Section: Introductionmentioning

confidence: 99%

Rapid, Simple and Low-Cost Reverse-Phase High-Performance Liquid Chromatography Tool for Estimation of Methotrexate: Testing Application in Standard Laboratory Bulk, Marketed Dosage Form, and Release kinetics in Nanoformulation

Tekade,

Sharma

2024

Ind. J. Pharm. Edu. Res

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Background: Methotrexate (MTX) is one of the utmost commonly prescribed drugs, which is official in the IP, BP, and USP. MTX is a potent drug that can cause serious side effects if not used in the correct quantity. Assaying MTX is vital to guarantee that the drug exists in the required concentration in the sample. Developing a simple, rapid, and cost-effective analytical tool for its estimation has always been a thrust area of research. In this line, this investigation reports a simple, rapid, and low-cost Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) method with an ultraviolet detection channel for estimating MTX in bulk samples. Materials and Methods: The technique employs an isocratic mobile phase comprising a readily available solvent system having 50 mM sodium acetate buffer (pH-3.6) and acetonitrile in 90:10, v/v, which flows through the column at a constant flow rate of 1.0 mL/min. A Thermo C-18 Column (5-micron, 250 mmx4.60 mm) was used as the stationary phase for separation. Considering the optimized chromatography parameters, sensitivity, and selectivity of a method for drugs, 307 nm was selected as the detection wavelength for the UV-visible detector. The validation studies were performed by fulfilling the requirements of ICH guidelines. Results and Conclusion: The method was found to be specific, linear (including intra-and inter-day precision), accurate, and precise for routine testing of MTX. The developed RP-HPLC method has been successfully applied to determine MTX in standard Laboratory Bulk samples and commercial Dosage Formulations, as well as to monitor the release kinetics of MTX from an in-house developed Nanoformulation. The developed method represents a valuable tool in the laboratory analysis and monitoring of MTX during routine quality control testing and toxicity studies.

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“…The application of an electrochemical probe for CLZ detection is highly limited by the electroactive functional groups to be oxidized or reduced in a proper potential. Although, the optical‐based methods do not have the reported limitations of the separation‐based techniques, however, they have their limitations including low stability, reproducibility, the high interfering effect of the coexisting agents, etc 13–16 Usually, clinics require simple methods with low pretreatment steps to quantify various biomolecules or pharmaceuticals in real samples 17 . Fluorescence‐based probes are considered a facile method for the measurement of biomarkers, pharmaceuticals, and environmental molecules and ions 18–21 .…”

Section: Introductionmentioning

confidence: 99%

“…Although, the optical-based methods do not have the reported limitations of the separation-based techniques, however, they have their limitations including low stability, reproducibility, the high interfering effect of the coexisting agents, etc [13][14][15][16] Usually, clinics require simple methods with low pretreatment steps to quantify various biomolecules or pharmaceuticals in real samples. 17 Fluorescence-based probes are considered a facile method for the measurement of biomarkers, pharmaceuticals, and environmental molecules and ions. [18][19][20][21] The fluorescence-based methods are usually affected by environmental conditions, especially in biological media.…”

mentioning

confidence: 99%

A versatile ratiometric fluorescence nanoprobe for the determination of clonazepam in patients' plasma samples

Abbasi,

Jouyban,

Ranjbar

et al. 2024

J of Molecular Recognition

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Despite the necessity of the study of therapeutic drug monitoring of clonazepam (CLZ), there are only a few fast detection methods available for determining CLZ in biological media. This study aims to develop a cost‐effective and ratiometric probe for the quantification of CLZ in plasma samples. Fluorescent polydopamine nanoparticles were produced through a self‐polymerization process at a pH of 8.5. Rhodamine B molecules were employed as a fluorescent reference material, emitting stable fluorescence in the visible range. The fabricated probe exhibited a specific detection capability for CLZ. The fluorescence emission of the probe was enhanced in two concentration ranges: from 50 ng/mL to 1.0 μg/mL and from 1.0 to 15.0 μg/mL with a lower limit of quantification of 50 ng/mL, indicating the sensitivity of the probe for detecting CLZ plasma levels. The accuracy of the probe is favorable which could be recommended for CLZ monitoring in the biological media. Furthermore, this probe is highly specific towards CLZ in the presence of various interfering agents which is mainly caused by its ratiometric nature. The developed platform showed high reliability in quantifying CLZ concentrations in patients' plasma samples. Hence, the fabricated probe could be recommended as a reliable method for the routine detection of CLZ in clinical settings.

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Ultrasensitive fluorescence detection of antitumor drug methotrexate based on a terbium-doped silica dendritic probe

Abstract: A novel strategy was developed for the detection of methotrexate (MTX) via the quenching effect of MTX on the fluorescence intensity of terbium-doped dendritic silica particles (Tb@KCC-1). The fluorescence intensity...

Cited by 10 publications

References 48 publications

Functional Group-Assisted Fluorescence Sensing Platform for Nanomolar-Level Detection of an Antineoplastic Drug and a Neurotransmitter from Environmental Water and Human Biofluids

Functional Group-Assisted Fluorescence Sensing Platform for Nanomolar-Level Detection of an Antineoplastic Drug and a Neurotransmitter from Environmental Water and Human Biofluids

Rapid, Simple and Low-Cost Reverse-Phase High-Performance Liquid Chromatography Tool for Estimation of Methotrexate: Testing Application in Standard Laboratory Bulk, Marketed Dosage Form, and Release kinetics in Nanoformulation

A versatile ratiometric fluorescence nanoprobe for the determination of clonazepam in patients' plasma samples

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