Ultrasensitive immuno-PCR for detecting aflatoxin B1 based on magnetic separation and barcode DNA

Li, Zhi; Yang, Chuqin; Lu, Wenying; Zhang, Chu; Zhang, Jingwen; Li, Ming; Wang, Qingjiang

doi:10.1016/j.foodcont.2022.109028

Cited by 6 publications

(3 citation statements)

References 34 publications

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“…In the work of Chen et al., the immunocomplexes of OTA antigen‐MNPs/mAb‐barcode DNA‐AuNPs can be dissociated by the addition of dithiothreitol; consequently, the disassembled barcode DNAs triggered the CHA reaction, thereby substantially enhancing the signal amplification (Figure 6e) (Chen et al., 2020). A comparable strategy was performed for AFB 1 detection, wherein the dissociated barcode DNA was used to initiate real‐time polymerase chain reaction amplification, resulting in a LOD of 0.01 pg/mL which was 480‐fold lower than that achieved using ELISA (Li, Yang et al., 2022). Instead of only utilizing Apts or Abs as the bioreceptors, the combination of both bioreceptors to prepare a fluorometric oligonucleotide immunoassay would be beneficial to obtain ultrasensitive results (Li, Han et al., 2022).…”

Section: Roles and Applications Of Mnps In Mycotoxin Detectionmentioning

confidence: 99%

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Gao,

Zhou,

Zhang

et al. 2023

Comp Rev Food Sci Food Safe

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Mycotoxin contamination of food crops is a global challenge due to their unpredictable occurrence and severe adverse health effects on humans. Therefore, it is of great importance to develop effective tools to prevent the accumulation of mycotoxins through the food chain. The use of magnetic nanoparticle (MNP)‐assisted biosensors for detecting mycotoxin in complex foodstuffs has garnered great interest due to the significantly enhanced sensitivity and accuracy. Within such a context, this review includes the fundamentals and recent advances (2020–2023) in the area of mycotoxin monitoring in food matrices using MNP‐based aptasensors and immunosensors. In this review, we start by providing a comprehensive introduction to the design of immunosensors (natural antibody or nanobody, random or site‐oriented immobilization) and aptasensors (techniques for aptamer selection, characterization, and truncation). Meanwhile, special attention is paid to the multifunctionalities of MNPs (recoverable adsorbent, versatile carrier, and signal indicator) in preparing mycotoxin‐specific biosensors. Further, the contribution of MNPs to the multiplexing determination of various mycotoxins is summarized. Finally, challenges and future perspectives for the practical applications of MNP‐assisted biosensors are also discussed. The progress and updates of MNP‐based biosensors shown in this review are expected to offer readers valuable insights about the design of MNP‐based tools for the effective detection of mycotoxins in practical applications.

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Section: Roles and Applications Of Mnps In Mycotoxin Detectionmentioning

confidence: 99%

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Gao,

Zhou,

Zhang

et al. 2023

Comp Rev Food Sci Food Safe

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“…To reduce the economic losses and health risks caused by AFB1, simple, rapid, and effective detection methods are a critical part of the process. In recent years, many reported methods have been used for the detection of AFB1 mycotoxins in agricultural products, such as HPLC [ 7 ], LC-MS [ 8 ], colorimetric immunoassay [ 9 ], electrochemistry [ 10 ], chemiluminescence [ 11 ], electrochemiluminescence methods [ 12 ], surface plasmon resonance [ 13 ], surface-enhanced Raman scattering [ 14 ], immuno-PCR [ 15 , 16 ], etc. At the same time, immunological methods with high specificity for antibody-antigen recognition have been developed and applied in various fields due to their excellent performance [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Production of AFB1 High-Specificity Monoclonal Antibody by Three-Stage Screening Combined with the De-Homologation of Antibodies and the Development of High-Throughput icELISA

Pang,

Liu,

Chen

et al. 2023

Toxins

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To achieve accurate detection of AFB1 toxin content in agricultural products and avoid false-positive rates in the assays, the specificity of mAbs is critical. We improved the specificity of the prepared monoclonal antibodies by modifying the traditional limiting dilution subcloning method. The traditional finite dilution method was modified with three-stage screening (the trending concentration of standards used in the screening is low–high–low) to achieve high specificity in pre-cell screening and increased the number of subclones to 10 to achieve the de-homologation of antibodies. A modified limiting dilution obtained a highly specific AFB1 monoclonal cell line, ZFG8, with a 50% inhibition concentration (IC50) of 0.3162 ng/mL. Notably, it exhibited the highest specificity compared to anti-AFB1 monoclonal antibodies prepared by other investigators. The maximum cross-reactivity of the mAb with structural analogues for AFB2, AFG1, AFG2, and AFM1 was 0.34%. The results showed that this type of screening improves the monoclonal antibodies’ specificity. Based on this ZFG8 monoclonal antibody, an icELISA assay was established with an IC50 of 0.2135 ng/mL for AFB1. The limit of the linear detection range of icELISA is 0.0422–1.29267 ng/mL with reasonable specificity and precision. The recoveries of AFB1 in samples of corn flour and wheat meal ranged from 84 to 107%, with CVs below 9.3%. The recoveries of structural analogues (AFB2, AFM1, AFG1, and AFG2) were less than 10% in both corn flour and wheat meal. The results showed that the prepared AFB1 monoclonal antibody could accurately and specifically recognize AFB1 residues in agricultural products while ignoring the effects of other structural analogues.

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“…These INPs are promising agents for magnetic hyperthermia [ 11 , 12 ]. Magnetic separation and biosensors based on INPs simplify laboratory diagnostics [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Nanoparticles of Bioactive Metals/Metal Oxides and Their Nanocomposites with Antibacterial Drugs for Biomedical Applications

et al. 2022

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The increasing appearance of new strains of microorganisms resistant to the action of existing antibiotics is a modern problem that requires urgent decision. A promising potential solution is the use of nanoparticles of bioactive metals and their oxides as new antibacterial agents, since they are capable of affecting pathogenic microorganisms by mechanisms different from the mechanisms of action of antibiotics. Inorganic nanoparticles possess a wide spectrum of antibacterial activity. These particles can be easily conjugated with drug molecules and become carriers in targeted drug-delivery systems. This paper discusses the benefits and prospects of the application of nanoparticles from metals and metal oxides and their nanocomposites with antibacterial drugs.

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Ultrasensitive immuno-PCR for detecting aflatoxin B1 based on magnetic separation and barcode DNA

Cited by 6 publications

References 34 publications

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Production of AFB1 High-Specificity Monoclonal Antibody by Three-Stage Screening Combined with the De-Homologation of Antibodies and the Development of High-Throughput icELISA

Nanoparticles of Bioactive Metals/Metal Oxides and Their Nanocomposites with Antibacterial Drugs for Biomedical Applications

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