Ultrasensitive mushroom-like electrochemical immunosensor for probing the activity of histone acetyltransferase

Faraday-Cage-Type Electrochemiluminescence Immunoassay: A Rise of Advanced Biosensing Strategy

“…In addition, we have developed an electrochemical biosensor based on a Faraday-cage-type immunoassay for the detection of histone acetyltransferase HAT p300 activity using MB&GO@AuNCs-Ab as the detection unit, which was composed of GO and a AuNC nanocomplex labeled by a detection antibody. 64 The detection range was established from 0.01 nM to 150 nM with a LOD of 0.0036 nM. The proposed immunosensor was also applied for screening HATrelated inhibitors anacardic acid and C646, and the corresponding IC 50 value was found to be 38.8 μM for anacardic acid and 12.0 μM for C646, respectively.…”

“…We further extended our research toward the detection of p300 in a HeLa cell lysate and spiked serum, demonstrating its practicality. In addition, we have developed an electrochemical biosensor based on a Faraday-cage-type immunoassay for the detection of histone acetyltransferase HAT p300 activity using MB&GO@AuNCs-Ab as the detection unit, which was composed of GO and a AuNC nanocomplex labeled by a detection antibody . The detection range was established from 0.01 nM to 150 nM with a LOD of 0.0036 nM.…”

“…The detection range was established from 0.01 nM to 150 nM with a LOD of 0.0036 nM. The proposed immunosensor was also applied for screening HAT-related inhibitors anacardic acid and C646, and the corresponding IC 50 value was found to be 38.8 μM for anacardic acid and 12.0 μM for C646, respectively …”

“…The proposed immunosensor was also applied for screening HATrelated inhibitors anacardic acid and C646, and the corresponding IC 50 value was found to be 38.8 μM for anacardic acid and 12.0 μM for C646, respectively. 64 In fact, research similar to FCT-ECLIA has been growing recently through the use of unique 2-D nanomaterials to build detection units for developing novel biosensing strategies. Deng's group employed a GO-PEI-carbon quantum dot (CQD)-Au nanohybrid as an active probe to detect carbohydrate antigen 15−3 (CA15−3).…”

Photothermal-Induced Electrochemical Interfacial Region Regulation Enables Signal Amplification for Dual-Mode Detection of Ovarian Cancer Biomarkers

“…As a matter of fact, sensitivity is particularly restricted by the accessible distance between signal labels and the sensing interface, which is a great challenge in electrochemical immunosensors. , Generally, the electrochemical response only occurs when the electrochemically active material is in the vicinity of the outer Helmholtz plane (OHP), thereby the signal labels within the OHP are “effective” to generate the intense electrochemical signal. , Therefore, increasing the loading amount of signal labels and regulating the OHP region of the sensing interface are essential for signal amplification. Presently, the mainstream reports are mainly focused on developing a “Faraday cage”-type immunoassay, using two-dimensional graphene oxide (GO) nanosheets as carriers to enhance the signal amount and extend the OHP region. , In this state, GO nanosheets overlap on the electrode surface to form a ringed Faraday cage, where signal labels could directly involve in the electrochemical reaction and allow the electrons to freely transfer to the electrode interface. , Nevertheless, the distance between signal labels and the electrode surface is finite in the Faraday cage; thus, the OHP region is fixed and exhibits limited signal amplification. On this basis, an intelligent signal probe with interface regulation capability to shorten the OHP can circumvent these difficulties, resulting in the improved accessibility of the sensing interface and increased loading amounts of signal tags to realize further signal amplification.…”