Ultrasensitive Nanoimmunosensor by coupling non-covalent functionalized graphene oxide platform and numerous ferritin labels on carbon nanotubes

Akter, Rashida; Jeong, Bongjin; Choi, Jong-Soon; Rahman, Md. Aminur

doi:10.1016/j.bios.2016.01.035

Cited by 57 publications

(23 citation statements)

References 43 publications

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“…It should be noted that this non-covalent functionalization was established by irreversible adsorption on the GO surface where the pyrenyl strongly interacts through π-stacking. The detection of CA15-3 was based on the enhanced bioelectrocatalytic reduction of H 2 O 2 in the presence of a high number of ferritins mediated by HQ at the GO/Py-COOH-modified electrode [29]. As Table 1 shows, a wide linear range from 0.05 to 100 U·mL −1 CA15-3 was obtained, and the developed immunosensor was applied to the analysis of serum with good results.…”

Section: Carbon Nanotubes Used As Carriers For Signal Elements In Elementioning

confidence: 98%

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

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Growing demand for developing ultrasensitive electrochemical bioassays has led to the design of numerous signal amplification strategies. In this context, carbon-based nanomaterials have been demonstrated to be excellent tags for greatly amplifying the transduction of recognition events and simplifying the protocols used in electrochemical biosensing. This relevant role is due to the carbon-nanomaterials' large surface area, excellent biological compatibility and ease functionalization and, in some cases, intrinsic electrochemistry. These carbon-based nanomaterials involve well-known carbon nanotubes (CNTs) and graphene as well as the more recent use of other carbon nanoforms. This paper briefly discusses the advantages of using carbon nanostructures and their hybrid nanocomposites for amplification through tagging in electrochemical biosensing platforms and provides an updated overview of some selected examples making use of labels involving carbon nanomaterials, acting both as carriers for signal elements and as electrochemical tracers, applied to the electrochemical biosensing of relevant (bio)markers.

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Section: Carbon Nanotubes Used As Carriers For Signal Elements In Elementioning

confidence: 98%

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

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“…The nanostructure-based immunosensor was developed by applying the non-covalent functionalization of GO with 1-pyrenecarboxylic acid as a modified electrode interface for the immobilization of a primary antibody (Ab 1 ) against the analyte [ 90 ]. Pre-treated GE were modified with a SAM of cysteamine, and the remaining empty places on the electrode were blocked with 2-mercaptoethanol.…”

Section: Nanomaterials/nanoparticles-based Electrochemical Biosensmentioning

confidence: 99%

“…After the activation of MWCNTs, nanotubes were covalently modified by polyclonal anti-CA15-3 Ab 2 and ferritin. CA15-3 was detected through an enhanced bioelectrocatalytic reduction of H 2 O 2 mediated by HQ at the immunosensor-offered LOD of 0.01 U/mL in human serum samples using DPV [ 90 ] (Approach 3).…”

Section: Nanomaterials/nanoparticles-based Electrochemical Biosensmentioning

confidence: 99%

Electrochemical Nanobiosensors for Detection of Breast Cancer Biomarkers

Gajdošová

Lorencová

Kasák

et al. 2020

Sensors

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This comprehensive review paper describes recent advances made in the field of electrochemical nanobiosensors for the detection of breast cancer (BC) biomarkers such as specific genes, microRNA, proteins, circulating tumor cells, BC cell lines, and exosomes or exosome-derived biomarkers. Besides the description of key functional characteristics of electrochemical nanobiosensors, the reader can find basic statistic information about BC incidence and mortality, breast pathology, and current clinically used BC biomarkers. The final part of the review is focused on challenges that need to be addressed in order to apply electrochemical nanobiosensors in a clinical practice.

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“…PtNPs were attached to graphene using the bifunctional 1-methyl pyrene amine linker, and thiol-containing single-chain variable fragment antibodies (scFv) were immobilized on PtNPs. Akter et al [ 108 ] constructed a sandwich-type CA 15-3 immunosensor using GO/1-pyrenecarboxylic acid (Py-COOH) as sensor probe and MWCNT-supported ferritin as labels. GO/Py-COOH was deposited on cysteamine (Cys) self-assembled monolayer (SAM) modified gold electrode, and anti-CA 15-3 antibodies were immobilized on both GO/Py-COOH and MWCNT/ferritin labels.…”

Section: Detection Of Cancer and Disease Biomarkersmentioning

confidence: 99%

Carbon Nanomaterial Based Biosensors for Non-Invasive Detection of Cancer and Disease Biomarkers for Clinical Diagnosis

Pasinszki

Krebsz

Tung

et al. 2017

Sensors

146

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The early diagnosis of diseases, e.g., Parkinson’s and Alzheimer’s disease, diabetes, and various types of cancer, and monitoring the response of patients to the therapy plays a critical role in clinical treatment; therefore, there is an intensive research for the determination of many clinical analytes. In order to achieve point-of-care sensing in clinical practice, sensitive, selective, cost-effective, simple, reliable, and rapid analytical methods are required. Biosensors have become essential tools in biomarker sensing, in which electrode material and architecture play critical roles in achieving sensitive and stable detection. Carbon nanomaterials in the form of particle/dots, tube/wires, and sheets have recently become indispensable elements of biosensor platforms due to their excellent mechanical, electronic, and optical properties. This review summarizes developments in this lucrative field by presenting major biosensor types and variability of sensor platforms in biomedical applications.

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Ultrasensitive Nanoimmunosensor by coupling non-covalent functionalized graphene oxide platform and numerous ferritin labels on carbon nanotubes

Cited by 57 publications

References 43 publications

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Electrochemical Nanobiosensors for Detection of Breast Cancer Biomarkers

Carbon Nanomaterial Based Biosensors for Non-Invasive Detection of Cancer and Disease Biomarkers for Clinical Diagnosis

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