Ultrasensitive quantitation of Paraquat based on a small molecule-induced dual-cycle amplification strategy

Exploring the Effect of Steric Hindrance on Trans-cleavage Activity of CRISPR-cas12a for Ultrasensitive SERS Detection of P53 DNA

“…7 In the case of Cas12a, for example, its trans-cleavage is multiple turnover that nonspecifically cleaves single-stranded DNA (ssDNA) into 2−4 oligonucleotides at cleavage efficiencies in excess of 1,200 per second. 8,9 Due to the unique cleavage properties of Cas12a combined with its programmability in guiding RNA, 10,11 it has been widely used for the detection of a variety of targets, including in vivo viral RNA, 12 various small molecules, 13,14 a wide range of proteins, 15,16 and some cancer markers. 17 In recent years, factors affecting the cleavage activity of cas12a have been explored in order to unlock its full potential.…”

“…The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas system plays an important role in gene editing systems, and its good prospects have received increasing attention in imaging, disease diagnosis, and treatment. − Among them, CRISPR type V and VI RNA-guided nucleic acid endonucleases, including Cas12a, Cas12b, Cas12c, Cas12h, Cas13b, and Cas14, − are not only capable of specific recognition and cis-cleavage of targets but also have the ability to nonspecifically trans-cleavage (also known as collateral cleavage) . In the case of Cas12a, for example, its trans-cleavage is multiple turnover that nonspecifically cleaves single-stranded DNA (ssDNA) into 2–4 oligonucleotides at cleavage efficiencies in excess of 1,200 per second. , Due to the unique cleavage properties of Cas12a combined with its programmability in guiding RNA, , it has been widely used for the detection of a variety of targets, including in vivo viral RNA, various small molecules, , a wide range of proteins, , and some cancer markers …”