Ultrasensitive quantitative detection of small molecules with rapid lateral-flow assay based on high-affinity bifunctional ligand and magnetic nanolabels

Znoyko, S.L.; Орлов, А. В.; Pushkarev, A.V.; Mochalova, Elizaveta N.; Guteneva, N.V.; Lunin, A. V.; Nikitin, Maxim P.; Никитин, П. И.

doi:10.1016/j.aca.2018.07.012

Cited by 52 publications

(30 citation statements)

References 44 publications

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“…The obtained data show optimization and validation of all stages of the developed magnetic lateral flow assay for quantitative and ultrasensitive express-detection of free thyroxine [1] . The assay was performed in an original competitive format with streptavidin on the test line (TL) of a lateral flow test strip and biotinylated thyroxin (T4-bt) competing with free thyroxin for the limited amount of antibody binding sites in sample solution.…”

Section: Introductionmentioning

confidence: 81%

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Data on characterization and validation of assays for ultrasensitive quantitative detection of small molecules: Determination of free thyroxine with magnetic and interferometric methods

et al. 2018

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The presented data refer to optimization and quantitative characterization of a rapid lateral flow assay based on high-affinity bifunctional ligand and magnetic nanolabels, which was developed for detection of small molecules of thyroid hormones. The results were obtained by several techniques, including the magnetic particle quantification method, spectral-correlation interferometry and spectral-phase interferometry, dynamic light scattering, enzyme linked immunosorbent assay. The long-term stability of “antibody – magnetic nanoparticle” conjugates is shown. The assay specificity is confirmed, and verification of successful combination of magnetic particles and antibodies is demonstrated. The kinetic and equilibrium dissociation constants are determined for interactions between thyroxine and monoclonal antibodies. The obtained data could be used for design of other platforms for detection of small molecules.

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Section: Introductionmentioning

confidence: 81%

“…5 Dependence of the registered magnetic signal upon antigen concentration in the presence (dash line – see details in Fig. 4 b in [1] ) and absence (dots) of T4-bt conjugate in the sample. …”

Section: Specificity Of the Assaymentioning

confidence: 99%

Data on characterization and validation of assays for ultrasensitive quantitative detection of small molecules: Determination of free thyroxine with magnetic and interferometric methods

et al. 2018

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“…MPQ approach is robust and permits registration of minute magnetic signals in the presence of strong noise, providing high sensitivity and precision. 55,56 The Nikitin group developed several MPQ based platforms for quantitative multiplex magnetic immunoassays for different cancer markers, such as prostate-specific antigen (PSA), using the 50 nm or 100 nm superparamagnetic microspheres conjugated to antibodies. The platforms include a) one-run tests on 3D porous filters where the signal was read out from the entire volume of the nontransparent 3D fiber filters employed as solid phase for sandwich immunoassays, with LOD of 12 pg/ml and the dynamic range Nebu et al used a different approach to ensure targeting of the biofunctionalized MNPs -BMNPs to the cells while achieving the theranostic effect in parallel.…”

Section: Application Of Magnetic Nanoarchitectures For Cancer Sensingmentioning

confidence: 99%

Magnetic nanoarchitectures for cancer sensing, imaging and therapy

2019

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“…Conjugation of any chemical substances including protein molecules, aptamers, and other nonmagnetic materials onto MNPs can hinder or even block this Brownian relaxation. 23 , 27 , 38 − 45 As a result, this binding event causes a phase lag between magnetic moments and external AC fields (except at very low frequencies), and thus, weaker dynamic magnetic responses are observed from clustered MNPs. By exploiting this unique property of Brownian relaxation-dominant MNPs, researchers have applied it along with the MPS platform for the detection of H1N1 virus, 39 thrombin, 38 staphylococcal toxins, 20 SARS-CoV-2, 23 , 46 botulinum neurotoxins A, B, and E, 21 and so forth.…”

Section: Introductionmentioning

confidence: 99%

One-Step, Wash-free, Nanoparticle Clustering-Based Magnetic Particle Spectroscopy Bioassay Method for Detection of SARS-CoV-2 Spike and Nucleocapsid Proteins in the Liquid Phase

Chugh

Krishna

et al. 2021

ACS Appl. Mater. Interfaces

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With the ongoing global pandemic of coronavirus disease 2019 (COVID-19), there is an increasing quest for more accessible, easy-to-use, rapid, inexpensive, and high-accuracy diagnostic tools. Traditional disease diagnostic methods such as qRT-PCR (quantitative reverse transcription-PCR) and ELISA (enzyme-linked immunosorbent assay) require multiple steps, trained technicians, and long turnaround time that may worsen the disease surveillance and pandemic control. In sight of this situation, a rapid, one-step, easy-to-use, and high-accuracy diagnostic platform will be valuable for future epidemic control, especially for regions with scarce medical resources. Herein, we report a magnetic particle spectroscopy (MPS) platform for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) biomarkers: spike and nucleocapsid proteins. This technique monitors the dynamic magnetic responses of magnetic nanoparticles (MNPs) and uses their higher harmonics as a measure of the nanoparticles’ binding states. By anchoring polyclonal antibodies (pAbs) onto MNP surfaces, these nanoparticles function as nanoprobes to specifically bind to target analytes (SARS-CoV-2 spike and nucleocapsid proteins in this work) and form nanoparticle clusters. This binding event causes detectable changes in higher harmonics and allows for quantitative and qualitative detection of target analytes in the liquid phase. We have achieved detection limits of 1.56 nM (equivalent to 125 fmole) and 12.5 nM (equivalent to 1 pmole) for detecting SARS-CoV-2 spike and nucleocapsid proteins, respectively. This MPS platform combined with the one-step, wash-free, nanoparticle clustering-based assay method is intrinsically versatile and allows for the detection of a variety of other disease biomarkers by simply changing the surface functional groups on MNPs.

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Ultrasensitive quantitative detection of small molecules with rapid lateral-flow assay based on high-affinity bifunctional ligand and magnetic nanolabels

Cited by 52 publications

References 44 publications

Data on characterization and validation of assays for ultrasensitive quantitative detection of small molecules: Determination of free thyroxine with magnetic and interferometric methods

Data on characterization and validation of assays for ultrasensitive quantitative detection of small molecules: Determination of free thyroxine with magnetic and interferometric methods

Magnetic nanoarchitectures for cancer sensing, imaging and therapy

One-Step, Wash-free, Nanoparticle Clustering-Based Magnetic Particle Spectroscopy Bioassay Method for Detection of SARS-CoV-2 Spike and Nucleocapsid Proteins in the Liquid Phase

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