2012
DOI: 10.1021/ac301033w
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Ultrasensitive Solution-Phase Electrochemical Molecular Beacon-Based DNA Detection with Signal Amplification by Exonuclease III-Assisted Target Recycling

Abstract: Taking advantage of the preferential exodeoxyribonuclease activity of exonuclease III in combination with the difference in diffusivity between an oligonucleotide and a mononucleotide toward a negatively charged ITO electrode, a highly sensitive and selective electrochemical molecular beacon (eMB)-based DNA sensor has been developed. This sensor realizes electrochemical detection of DNA in a homogeneous solution, with sensing signals amplified by an exonuclease III-based target recycling strategy. A hairpin-sh… Show more

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Cited by 195 publications
(141 citation statements)
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“…Also, the spatial hindrance effect of the electrode surface and the loss of configuration freedom caused by binding targets on the solution-electrode interface usually make these heterogeneous assays suffer from relatively low hybridization efficiency and enzyme kinetics compared to homogeneous assays . Therefore, faster and easier-to-use homogeneous electrochemical strategies have been developed for the detection of various targets (Xuan et al 2012;Xuan et al 2013;Zhang et al 2013b). Additionally, the substrate electrode is very important for the design and manufacture of homogeneous electrochemical sensor.…”
Section: Introductionmentioning
confidence: 99%
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“…Also, the spatial hindrance effect of the electrode surface and the loss of configuration freedom caused by binding targets on the solution-electrode interface usually make these heterogeneous assays suffer from relatively low hybridization efficiency and enzyme kinetics compared to homogeneous assays . Therefore, faster and easier-to-use homogeneous electrochemical strategies have been developed for the detection of various targets (Xuan et al 2012;Xuan et al 2013;Zhang et al 2013b). Additionally, the substrate electrode is very important for the design and manufacture of homogeneous electrochemical sensor.…”
Section: Introductionmentioning
confidence: 99%
“…For example, Hsing and colleagues have demonstrated solution-phase electrochemical strategies for sensitive detection of DNA and mercury ion (Xuan et al 2012;Xuan et al 2013). Very recently, our group also has developed highly sensitive homogeneous electrochemical biosensors for the detection of methyltransferase (Li et al 2015b) and human telomerase activity .…”
Section: Introductionmentioning
confidence: 99%
“…Many detection systems have been developed by making use of enzymes as amplifiers because of the low abundance of targets in natural environments. Amplificatory tools, such as polymerase (Manrao et al, 2012), ligase (Shen et al, 2012;Wee et al, 2012) and exonuclease (Ju et al, 2012;Xuan et al, 2012), in DNA detection offer exquisite sensitivity and are commonly used to follow target-probe hybridization. However, these enzyme-based assays are labor-intensive, require complex operation, and have a relatively high cost.…”
Section: Introductionmentioning
confidence: 99%
“…Through Exo III-assisted cycling reaction, a large amount of single-stranded DNAs can be acquired for signal amplification. However, most of the previous works using Exo III-assisted DNA cycling were involved in labeling procedures, such as tagging fluorophore for optical detection and tagging redox labels for electrochemical analysis (Hu et al, 2012;Gao and Li, 2014;Liu et al, 2014;Xuan et al, 2012).…”
Section: Introductionmentioning
confidence: 99%