Ultrasensitive surface-enhanced Raman scattering detection of alkaline phosphatase

Dong, Jian; Li, Yuan; Zhang, Mingyue; Li, zhang; Yan, Tianyu; Qian, Weiping

doi:10.1039/c4ay01885k

Cited by 38 publications

(16 citation statements)

References 17 publications

(15 reference statements)

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“…Nile blue A is Raman active and they used it to replace nitro blue tetrazolium that is more commonly used. The oxidized form is Raman active and it can be reduced by the ALP substrate product effectively turning off signal …”

Section: Applications Of Nile Red and Nile Bluementioning

confidence: 99%

Nile Red and Nile Blue: Applications and Syntheses of Structural Analogues

Martinez

Henary

2016

Chemistry A European J

196

157

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Nile red and Nile blue are highly fluorescent and photostable organic dyes from the benzo[a]phenoxazine family. They have been used as histological stains for imaging lysosomes and lipids in vitro. The dyes' high quantum yields and solvent-dependent optical properties make them ideal scaffolds for the development of pH probes and local polarity indicators. Reviews of the literature in this area are scarce with only one review ever published in 2006. It has been 10 years since and the field has evolved. This review aims to expand upon topics covered by the previous reviewers and to report on recent advances in the literature. As authors, we hope to convey a sense of scope and to spark renewed interest in this useful niche of dye chemistry.

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Section: Applications Of Nile Red and Nile Bluementioning

confidence: 99%

Nile Red and Nile Blue: Applications and Syntheses of Structural Analogues

Martinez

Henary

2016

Chemistry A European J

196

157

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“…It was displayed in Figure 2(i) that the Raman spectra of the NBA, NBA-labeled AgNWs, and NBA-labeled AgNW@ AuNPs were employed to explore the SERS effect of the AgNW@AuNPs. e aromatic ring vibrations resulted in the characteristic peak of NBA at 592 cm −1 [34]. Compared to the slight variation of the SERS signal of the 0.1 M NBA, the SERS signal of the NBA-labeled AgNW was lower than that of the NBA-labeled AgNW@AuNPs, which indicated that the AgNW@AuNPs could enhance the Raman signal intensity better.…”

Section: Characterization Of Ag Nanowires and Agnw@aunpsmentioning

confidence: 92%

SERS Platform Based on Bimetallic Au-Ag Nanowires-Decorated Filter Paper for Rapid Detection of miR-196ain Lung Cancer Patients Serum

Liu

Ran

et al. 2020

Journal of Chemistry

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Detecting microRNA (miRNA) biomarkers expression is of great significance for the diagnosis and treatment of lung cancer. Surface-enhanced Raman scattering (SERS) has achieved microRNA sensing for the diagnosis of primary liver cancers. In this work, we developed a SERS technology for the rapid detection of lung cancers-related miRNA (miR-196a) using bimetallic Au-Ag nanowire (AgNW@AuNPs) substrates coupled with the target hairpin DNA. The finite-difference time-domain simulation proved that a large number of “hot spots” were generated between the AgNW and AuNPs, which resulted in a huge enhancement of the signal of Raman reporters. Filter paper treated by hexadecenyl succinic anhydride hydrophobic and modified with AgNWs@AuNPs was used as capturing substrate. The detection limits of miR-196a in PBS and serum were as low as 96.58 aM and 130 aM, respectively. Studies on nonspecific sequence and single-base mismatch of miRNA demonstrated that SERS-based platform was highly selective, excellent uniform, and reproducible. Finally, the platform was used to show that the miR-196a expression in the serum of lung cancer patients was much higher than that in healthy people. The detection results indicated that the SERS platform had potential applications in cancer diagnosis and might be a viable alternative to the conventional miRNA detection method, the real-time polymerase chain reaction (RT-PCR) technology.

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“…The use of nanoparticles simultaneously with enzymes allowed for combining the functions of the carrier of active components of immunoassay (antibodies, antigens) and the enzymatic transformation of substrates [ 168 ]. The substrate of alkaline phosphatase, the so-called 5-bromo-4-chloro-3-indolyl phosphate (BCIP), in the immunoassay is hydrolyzed to the inorganic phosphate and sole active compound 5-bromo-4-chloro-3-indole (BCI) [ 169 , 170 ]. The resulting product of the reaction has characteristic bands detected during the SERS immunoassay.…”

Section: Recent Advances Of Raman Spectroscopy In Biosensingmentioning

confidence: 99%

Raman Scattering-Based Biosensing: New Prospects and Opportunities

et al. 2021

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The growing interest in the development of new platforms for the application of Raman spectroscopy techniques in biosensor technologies is driven by the potential of these techniques in identifying chemical compounds, as well as structural and functional features of biomolecules. The effect of Raman scattering is a result of inelastic light scattering processes, which lead to the emission of scattered light with a different frequency associated with molecular vibrations of the identified molecule. Spontaneous Raman scattering is usually weak, resulting in complexities with the separation of weak inelastically scattered light and intense Rayleigh scattering. These limitations have led to the development of various techniques for enhancing Raman scattering, including resonance Raman spectroscopy (RRS) and nonlinear Raman spectroscopy (coherent anti-Stokes Raman spectroscopy and stimulated Raman spectroscopy). Furthermore, the discovery of the phenomenon of enhanced Raman scattering near metallic nanostructures gave impetus to the development of the surface-enhanced Raman spectroscopy (SERS) as well as its combination with resonance Raman spectroscopy and nonlinear Raman spectroscopic techniques. The combination of nonlinear and resonant optical effects with metal substrates or nanoparticles can be used to increase speed, spatial resolution, and signal amplification in Raman spectroscopy, making these techniques promising for the analysis and characterization of biological samples. This review provides the main provisions of the listed Raman techniques and the advantages and limitations present when applied to life sciences research. The recent advances in SERS and SERS-combined techniques are summarized, such as SERRS, SE-CARS, and SE-SRS for bioimaging and the biosensing of molecules, which form the basis for potential future applications of these techniques in biosensor technology. In addition, an overview is given of the main tools for success in the development of biosensors based on Raman spectroscopy techniques, which can be achieved by choosing one or a combination of the following approaches: (i) fabrication of a reproducible SERS substrate, (ii) synthesis of the SERS nanotag, and (iii) implementation of new platforms for on-site testing.

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Ultrasensitive surface-enhanced Raman scattering detection of alkaline phosphatase

Cited by 38 publications

References 17 publications

Nile Red and Nile Blue: Applications and Syntheses of Structural Analogues

Nile Red and Nile Blue: Applications and Syntheses of Structural Analogues

SERS Platform Based on Bimetallic Au-Ag Nanowires-Decorated Filter Paper for Rapid Detection of miR-196ain Lung Cancer Patients Serum

Raman Scattering-Based Biosensing: New Prospects and Opportunities

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