Ultrasensitive transglutaminase based nanosensor for early detection of celiac disease in human

Gupta, Shagun; Kaushal, Ankur; Kumar, Ashok; Kumar, Dinesh

doi:10.1016/j.ijbiomac.2017.07.126

Cited by 44 publications

(23 citation statements)

References 29 publications

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“…Advancement of methods using biosensors for the brisk location of HEV in clinical, sustenance and ecological examples is the need of the hour. The research has been done to develop the amperometric sensor for quickly recognizing Streptococcus pyogenes causing rheumatic heart disease; and ultrasensitive transglutaminase based nano-sensor for early detection of celiac disease in human 127,128 . Additionally, consideration is required for more proficiency in planning of HEV vaccines development with new innovations to meet the future requirement.…”

Section: Resultsmentioning

confidence: 99%

Hepatitis E: Current Status in India and Other Asian Countries

Kumar¹,

Kamar²,

Kumar³

2019

J Pure Appl Microbiol

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Six types of hepatitis infections are accounted for the inflammation of liver in human throughout the world and assigned the letter A, B, C, D, E and G. Out of these, Hepatitis E, caused by Hepatitis E virus (HEV), currently categorized as a member of the genus Hepevirus in the family Hepeviridae. Hepatitis E is considered highly significant, on account of its predominance in both developed and developing nations, due to poor sanitation and cleanliness condition related to drinking water. This review focuses on general epidemiological study update of hepatitis E virus in India and rest of Asia for the selected period and also throws light on some diagnostic approaches available for the same. An extensive literature search was performed with published work and search in PubMed using term "HEV" combined with "country name" restricting the search publication between year 2008 to 2018 for India and 2013 to 2018 for the rest of Asia. The data was analyzed in detail to meet the requirements of the objectives. Study showed that in India the major causes of Hepatitis E was water contaminated with fecal matter. Reports from East and Southeast Asia signified the role of animal reservoir especially the pigs for HEV. Whereas in Indian neighboring countries like Nepal, Pakistan and Western Asia the major cases of Hepatitis E were associated with blood transfusion and pregnancy related cases. Majority of the diagnostic approaches used in India at medical facilities are based on anti-HEV IgM detection. However, the molecular based approaches played very crucial role in HEV detection. It is observed that the incidence of HEV is gradually expanding and its objective range is not only just developing countries, but also showing an enhancement. In overall, there is need for improvements in detection of HEV disease and methodologies used for the development of HEV vaccines.

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Section: Resultsmentioning

confidence: 99%

Hepatitis E: Current Status in India and Other Asian Countries

Kumar¹,

Kamar²,

Kumar³

2019

J Pure Appl Microbiol

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“…The reviewed GQD-sensing techniques and their biomedical applications are outlined in Figure 1. Since 2015, many investigational studies on GQD biosensors have made a giant step forward due to their aforementioned characteristics [67,[76][77][78]. These studies could specifically and sensitively detect target analytes by virtue of multiplexing electrodes such as screen-printed carbon electrodes (SPCE), screen-printed gold electrodes (SPGE) glassy carbon electrodes (GCE), etc.…”

Section: Approaches To Synthesize Gqds For Biomedical Sensorsmentioning

confidence: 99%

Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

190

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Due to the proliferative cancer rates, cardiovascular diseases, neurodegenerative disorders, autoimmune diseases and a plethora of infections across the globe, it is essential to introduce strategies that can rapidly and specifically detect the ultralow concentrations of relevant biomarkers, pathogens, toxins and pharmaceuticals in biological matrices. Considering these pathophysiologies, various research works have become necessary to fabricate biosensors for their early diagnosis and treatment, using nanomaterials like quantum dots (QDs). These nanomaterials effectively ameliorate the sensor performance with respect to their reproducibility, selectivity as well as sensitivity. In particular, graphene quantum dots (GQDs), which are ideally graphene fragments of nanometer size, constitute discrete features such as acting as attractive fluorophores and excellent electro-catalysts owing to their photo-stability, water-solubility, biocompatibility, non-toxicity and lucrativeness that make them favorable candidates for a wide range of novel biomedical applications. Herein, we reviewed about 300 biomedical studies reported over the last five years which entail the state of art as well as some pioneering ideas with respect to the prominent role of GQDs, especially in the development of optical, electrochemical and photoelectrochemical biosensors. Additionally, we outline the ideal properties of GQDs, their eclectic methods of synthesis, and the general principle behind several biosensing techniques.

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“…This is mainly due to its simplicity and low cost while maintaining sensitivity and selectivity . Actually, enzymatic immunosensors have been developed on nanostructured carbon electrodes or gold electrodes modified with transglutaminase, even with complicated architectures . However, simplicity and low cost remain as unmet needs in the diagnosis of the celiac disease.…”

Section: Figurementioning

confidence: 99%

Staple‐Based Paper Electrochemical Platform for Celiac Disease Diagnosis

et al. 2018

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A staple‐based electrochemical platform is proposed for the first time as a simple and low‐cost detection system for paper‐based devices. The system, which incorporates small and disposable stainless‐steel staples as electrodes, is combined with a paper strip. The staple acting as the working electrode is modified with carbon ink. The platform was carefully optimized with ferrocene carboxylic acid. As a proof‐of‐concept, it was employed for the enzymatic [horseradish peroxidase (HRP)‐based] immunoelectroanalytical detection of human tissue anti‐transglutaminase (anti‐tTG), which is a biomarker for celiac disease diagnosis. The intensity of the current from the electrochemical reduction of 3,3′,5,5′‐tetramethylbenzidine (an HRP substrate) was recorded chronoamperometrically at −0.2 V in different paper areas. A linear relationship between the current measured at 30 s and the logarithm of the concentration of anti‐tTG in the range comprised between 3 and 100 U mL−1 was obtained. Negative and positive controls produced expected values. Results demonstrated that the paper/staple combined platform is very convenient for the detection of electroactive analytes and other compounds that can be determined indirectly in bioassays.

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Ultrasensitive transglutaminase based nanosensor for early detection of celiac disease in human

Cited by 44 publications

References 29 publications

Hepatitis E: Current Status in India and Other Asian Countries

Hepatitis E: Current Status in India and Other Asian Countries

Applications of Graphene Quantum Dots in Biomedical Sensors

Staple‐Based Paper Electrochemical Platform for Celiac Disease Diagnosis

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