Ultrasensitive US Microvessel Imaging of Hepatic Microcirculation in                     the Cirrhotic Rat Liver

Zhang, Wei; Huang, Chengwu; Yin, Tinghui; Miao, Xiaoyan; Deng, Huan; Zheng, Rongqin; Ren, Jie; Chen, Shigao

doi:10.1148/radiol.220739

Cited by 7 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taking advantage of technical advances in signal acquisition and processing, ultrafast Doppler imaging (UFD) attains very high ultrasound (US) sampling speeds, with frame rates of up to 20 KHz. The resulting ultrasound imaging (USI) possesses the vascular sensitivity needed in cardiovascular, [ 19 , 20 ] hepatic, [ 21 ] nephrological, [ 22 ] and neurological research. [ 23 , 24 , 25 , 26 ] The Doppler signal intensity itself represents the instantaneous blood volume, hence UFD can capture both the vascular morphology and local hemodynamics of the organ.…”

Section: Introductionmentioning

confidence: 99%

Contrast Agent‐Free 3D Renal Ultrafast Doppler Imaging Reveals Vascular Dysfunction in Acute and Diabetic Kidney Diseases

Oh,

Lee,

Heo

et al. 2023

Advanced Science

View full text Add to dashboard Cite

To combat the irreversible decline in renal function associated with kidney disease, it is essential to establish non‐invasive biomarkers for assessing renal microcirculation. However, the limited resolution and/or vascular sensitivity of existing diagnostic imaging techniques hinders the visualization of complex cortical vessels. Here, a 3D renal ultrafast Doppler (UFD) imaging system that uses a high ultrasound frequency (18 MHz) and ultrahigh frame rate (1 KHz per slice) to scan the entire volume of a rat's kidney in vivo is demonstrated. The system, which can visualize the full 3D renal vascular branching pyramid at a resolution of 167 µm without any contrast agent, is used to chronically and noninvasively monitor kidneys with acute kidney injury (AKI, 3 days) and diabetic kidney disease (DKD, 8 weeks). Multiparametric UFD analyses (e.g., vessel volume occupancy (VVO), fractional moving blood volume (FMBV), vessel number density (VND), and vessel tortuosity (VT)) describe rapid vascular rarefaction from AKI and long‐term vascular degeneration from DKD, while the renal pathogeneses are validated by in vitro blood serum testing and stained histopathology. This work demonstrates the potential of 3D renal UFD to offer valuable insights into assessing kidney perfusion levels for future research in diabetes and kidney transplantation.

show abstract

Section: Introductionmentioning

confidence: 99%

Contrast Agent‐Free 3D Renal Ultrafast Doppler Imaging Reveals Vascular Dysfunction in Acute and Diabetic Kidney Diseases

Oh,

Lee,

Heo

et al. 2023

Advanced Science

View full text Add to dashboard Cite

show abstract

“…Liver fibrosis is also noted to be associated with an increased T 2a ( 37 , 38 ). Though pathophysiologically liver fibrosis is indeed associated with perfusion reduction ( 39 - 41 ), the PFm measured by standard IVIM could also have overestimated the extent of its reduction (or it could be a false positivity for the early-stage liver fibrosis cases). In the opposite direction, we noted that a higher liver iron content, and thus the associated shortening of T 2a / T 2 *, may be associated with a higher liver PFm ( 42 ).…”

mentioning

confidence: 99%

T2 relaxation time elongation of hepatocellular carcinoma relative to native liver tissue leads to an underestimation of perfusion fraction measured by standard intravoxel incoherent motion magnetic resonance imaging

Ma,

Wáng

2024

Quant Imaging Med Surg

View full text Add to dashboard Cite

“…Thanks to the development of plane wave‐based ultrafast ultrasound imaging, 11 a large amount of data can be acquired in a short time, which promotes the application of advanced spatiotemporal clutter filters, such as singular value decomposition (SVD) 12 . Thus, ultrafast power Doppler imaging (uPDI) has been proposed and developed to visualize the microvascular blood flow and microvascular morphology, such as tortuosity and density 13 . However, the resolution of uPDI is still limited owing to the ultrasound diffraction limit, and the blood flow velocity information cannot be obtained 14 …”

mentioning

confidence: 99%

“…12 Thus, ultrafast power Doppler imaging (uPDI) has been proposed and developed to visualize the microvascular blood flow and microvascular morphology, such as tortuosity and density. 13 However, the resolution of uPDI is still limited owing to the ultrasound diffraction limit, and the blood flow velocity information cannot be obtained. 14 Recently, with the injection of contrast agents microbubbles (MBs), ultrasound localization microscopy (ULM) was developed, 15,16 significantly improving the resolution by breaking the diffraction limit of ultrasound imaging.…”

mentioning

confidence: 99%

Evaluation of Early Diabetic Kidney Disease Using Ultrasound Localization Microscopy: A Feasibility Study

Zhang

Huang

Yang

et al. 2023

J of Ultrasound Medicine

View full text Add to dashboard Cite

ObjectiveThe purpose of this study is to detect the hemodynamic changes of microvessels in the early stage of diabetic kidney disease (DKD) and to test the feasibility of ultrasound localization microscopy (ULM) in early diagnosis of DKD.MethodsIn this study, streptozotocin (STZ) induced DKD rat model was used. Normal rats served as the control group. Conventional ultrasound, contrast‐enhanced ultrasound (CEUS), and ULM data were collected and analyzed. The kidney cortex was divided into four segments, which are 0.25–0.5 mm (Segment 1), 0.5–0.75 mm (Segment 2), 0.75–1 mm (Segment 3), and 1–1.25 mm (Segment 4) away from the renal capsule, respectively. The mean blood flow velocities of arteries and veins in each segment were separately calculated, and also the velocity gradients and overall mean velocities of arteries and veins. Mann–Whitney U test was used for comparison of the data.ResultsQuantitative results of microvessel velocity obtained by ULM show that the arterial velocity of Segments 2, 3, and 4, and the overall mean arterial velocity of the four segments in the DKD group are significantly lower than those in the normal group. The venous velocity of Segment 3 and the overall mean venous velocity of the four segments in the DKD group are higher than those in the normal group. The arterial velocity gradient in the DKD group is lower than that in the normal group.ConclusionULM can visualize and quantify the blood flow and may be used for early diagnosis of DKD.

show abstract

Ultrasensitive US Microvessel Imaging of Hepatic Microcirculation in the Cirrhotic Rat Liver

Cited by 7 publications

References 35 publications

Contrast Agent‐Free 3D Renal Ultrafast Doppler Imaging Reveals Vascular Dysfunction in Acute and Diabetic Kidney Diseases

Contrast Agent‐Free 3D Renal Ultrafast Doppler Imaging Reveals Vascular Dysfunction in Acute and Diabetic Kidney Diseases

T2 relaxation time elongation of hepatocellular carcinoma relative to native liver tissue leads to an underestimation of perfusion fraction measured by standard intravoxel incoherent motion magnetic resonance imaging

Evaluation of Early Diabetic Kidney Disease Using Ultrasound Localization Microscopy: A Feasibility Study

Contact Info

Product

Resources

About