Ultrasmall Cu₂O@His Nanozymes with RONS Scavenging Capability for Anti-inflammatory Therapy

Abstract

High concentrations of reactive oxygen and nitrogen species (RONS) are key characteristics of inflammatory sites. Scavenging RONS at the site of inflammation is an effective therapeutic strategy. This study introduces ultrasmall Cu 2 O@His nanoparticles with RONS-scavenging ability for the treatment of inflammatory bowel disease (IBD) in mice. The strong coordination between the nitrogen atom in histidine (His) and copper enhances the dispersion and stability of Cu 2 O@His. Due to their small size and large surface area, Cu 2 O@His exhibits outstanding RONS-clearing ability. Importantly, Cu 2 O@His can target mitochondrial sites and repair damaged mitochondria. With excellent dispersion and scavenging RONS ability, Cu 2 O@His demonstrates good efficacy in treating mouse IBD. This work provides a new paradigm for developing nanozymes with an ultrasmall size and multiple scavenging RONS abilities.

Ultrasmall CuMn-His Nanozymes with Multienzyme Activity at Neutral pH: Construction of a Colorimetric Sensing Array for Biothiol Detection and Disease Identification

“…The valence allocation of Cu within CuMn-His is delineated through Cu 2p spectroscopy, wherein Cu + species comprise 79.16%, with Cu 2+ constituting the remaining 20.84% (Figure H) . In the absence of Mn supplementation during the synthesis process, the resultant product was as Cu 2 O@His, wherein the predominant valence state of Cu was primarily +1, as depicted in Figure S3 . This observation suggests the occurrence of Cu + oxidation facilitated by Mn species with higher valence states during the fabrication of CuMn-His.…”

“…The zeta potential of MnOOH was measured at −2.2 ± 0.8 mV, while that of CuMn-His was −29.3 ± 2.5 mV (Figure F). This substantial difference is attributed to the increased negative charge on CuMn-His, which results from the adsorption of His onto the surface of CuMn oxides via the N–Cu bond, thereby exposing carboxylic acid groups . The stronger charge repulsion leads to the formation of an ultrasmall particle size.…”

Bletilla striata Polysaccharide-/Chitosan-Based Self-Healing Hydrogel with Enhanced Photothermal Effect for Rapid Healing of Diabetic Infected Wounds via the Regulation of Microenvironment

“…To achieve a more comprehensive PTA, the design of inorganic–organic PTAs has emerged, aiming to integrate exceptional photothermal effects into high biocompatibility. Copper oxide nanoparticles (CuO NPs) are particularly interesting due to their positive effects on anti-inflammatory, angiogenesis and wound healing, in part because copper is an important trace element. , Polydopamine (PDA) is one of the most promising organic PTAs because of its excellent photothermal conversion efficiency and biocompatibility . Moreover, PDA possesses multiple functionalities, including drug-carrying and adhesion capabilities .…”

Biomimetic Integrated Nanozyme for Flare and Recurrence of Gouty Arthritis

“…Their popularity stems from high activity levels, cost-effectiveness, and storage stability [11] , [12] , [13] , [14] , [15] . For example, applications include the treatment of antioxidant stress through catalase (CAT) and superoxide dismutase (SOD) activities [16] , [17] , [18] , [19] , [20] , [21] , biosensor detection based on peroxidase (POD) and glucose oxidase (Gox) activity [22] , [23] , [24] , [25] , as well as cancer photokinetics and chemokinetics utilizing CAT and POD activity [26] , [27] , [28] , [29] , [30] . Reactive oxygen species (ROS), including superoxide anion radicals (⋅O 2 − ), hydrogen peroxide (H 2 O 2 ) and hydroxyl radicals (·OH − ), are partially reduced oxygen metabolites with strong oxidative capabilities.…”