Ultrasonic Cell Grinder Extraction of Cyperenoic Acid from Crotonis Crassifolii Radix and the Activity Assay of the Extract

Abstract

It is of great significance to achieve a high‐value usage of Chinese medicine residues. This study developed a green ultrasonic cell grinder extraction (UCGE) method has been developed for cyperenoic acid extraction from water decocted Crotonis Crassifolii Radix. The response surface methodology was introduced for the experimental design. The optimum conditions were as follows: 52 % ethanol concentration, 28 : 1 mL/g ratio of solution‐to‐solid, 500 W extraction power, and 28 min extraction time. Anti‐inflammatory test was performed to investigate the medicinal uses of the extract from the herb residue. The microstructure of the extracted samples was also investigated using scanning electron microscopy to understand the extraction mechanism. A higher extraction yield of 1.016 mg/g and better anti‐inflammatory activity were obtained under UCGE compared to a conventional solvent extraction. Meanwhile, the sample structure was seriously damaged by UCGE. The results show that UCGE is a promising green and efficient technology for extracting functional components, and has great potential in recycling waste resources.

Characterization of the chemical constituents of Croton crassifolius Geisel extract and plasma pharmacokinetics of 6 terpenoids after oral administration by UPLC‐Q/TOF‐MS

“…The contents of the six terpenoids of the CCG samples were investigated by an external standard method, 25 using the time of flight mass spectrometry (TOF-MS) primary quantification strategy with consistent mass spectrometry conditions for qualitative analysis, except that the chromatographic elution time was shortened. Indeed, this method has been validated to ensure that the specificity, linearity and sensitivity, precision and accuracy, repeatability, and stability conformed to the guidelines for quantitative analysis of TCM in the Chinese Pharmacopoeia (2020).…”

“…The most intense peaks during collision energy optimization were m/z that the addition of formic acid to the mobile phase could improve the signal response of the analytes, the mobile phase of 0.1% formic acid acetonitrile-0.1% formic acid water system was selected. In addition, the peak shapes and resolution under each condition were evaluated by examining various column temperatures (25,30,35, and 40 C) and different flow rates (0.1, 0.2, 0.3, and 0.4 mL/min). Finally, the flow rate was set to 0.4 mL/min and the column temperature to 40 C.…”