Ultrasonic Characterization of Biological Cells

Bereiter‐Hahn, Jürgen; Blasé, Christopher

doi:10.1201/9780203501962.ch12

Cited by 7 publications

(8 citation statements)

References 56 publications

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“…Subsequent jet-streams can arise with velocities as large as 800 m/sec. d ) The energy loss at acoustic impedance interfaces between tissues (Table 1) and even at sub-cellular structures (Bereiter-Hahn and Blase 2003; Lemor et al 2004) by refraction and diffraction contribute to the biological effect of ESW (Table 2). …”

Section: Introductionmentioning

confidence: 99%

Impact of extracorporeal shock waves on the human skin with cellulite: A case study of an unique instance

Kühn¹

2008

CIA

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In this case study of an unique instance, effects of medium-energy, high-focused extracorporeal generated shock waves (ESW) onto the skin and the underlying fat tissue of a cellulite affl icted, 50-year-old woman were investigated. The treatment consisted of four ESW applications within 21 days. Diagnostic high-resolution ultrasound (Collagenoson) was performed before and after treatment. Directly after the last ESW application, skin samples were taken for histopathological analysis from the treated and from the contra-lateral untreated area of skin with cellulite. No damage to the treated skin tissue, in particular no mechanical destruction to the subcutaneous fat, could be demonstrated by histopathological analysis. However an astounding induction of neocollageno-and neoelastino-genesis within the scaffolding fabric of the dermis and subcutis was observed. The dermis increased in thickness as well as the scaffolding within the subcutaneous fat-tissue. Optimization of critical application parameters may turn ESW into a noninvasive cellulite therapy.

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Section: Introductionmentioning

confidence: 99%

Impact of extracorporeal shock waves on the human skin with cellulite: A case study of an unique instance

Kühn¹

2008

CIA

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“…Various SAM techniques have been developed for the mechanoelastic quantification of biological materials on a microscopic cell and tissue level [24,31]. Detailed descriptions of the fundamental principles of acoustic microscopy have been described elsewhere [26,31].…”

Section: Scanning Acoustic Microscopy Techniquesmentioning

confidence: 99%

“…Scanning Acoustic Microscopy (SAM) is a robust approach for the study of viscoelastic properties of biological tissues and single cells [24,25]. Employing very short focused ultrasound pulses allows for the simultaneous determination of cell thickness, sound velocity, viscoelasticity, and density [26 -28].…”

Section: Introductionmentioning

confidence: 99%

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High-Frequency Time-Resolved Scanning Acoustic Microscopy for Biomedical Applications

Anastasiadis¹,

Zinin²

2018

TONIJ

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High-frequency focused ultrasound has emerged as a powerful modality for both biomedical imaging and elastography. It is gaining more attention due to its capability to outperform many other imaging modalities at a submicron resolution. Besides imaging, high-frequency ultrasound or acoustic biomicroscopy has been used in a wide range of applications to assess the elastic and mechanical properties at the tissue and single cell level. The interest in acoustic microscopy stems from the awareness of the relationship between biomechanical and the underlying biochemical processes in cells and the vast impact these interactions have on the onset and progression of disease. Furthermore, ultrasound biomicroscopy is characterized by its non-invasive and non-destructive approach. This, in turn, allows for spatiotemporal studies of dynamic processes without the employment of histochemistry that can compromise the integrity of the samples. Numerous techniques have been developed in the field of acoustic microscopy. This review paper discusses high-frequency ultrasound theory and applications for both imaging and elastography.

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“…And in the measurement, the particle size can range from nanometers to tens of micrometers, which might be suitable for human erythrocyte cells with size from 6 to 9 µm. One of the promising developments of the acoustoelectric method is used for early diagnostics [19]. We, therefore, could study the ultrasonic biological effects of cells in real time by inserting the detecting ultrasonic probe continuously during ultrasonic irradiation.…”

Section: Introductionmentioning

confidence: 99%

Study on the dielectric properties of biological cells using the electrokinetic method

Cheng

Zhong

Zhang

et al. 2012

IEEJ Transactions Elec Engng

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Different from hard particles with an ordinary surface structure, a biological cell is a soft particle which can be described as a hard particle covered with an ion-penetrable surface layer of polyelectrolytes. In this paper, according to the electrokinetic theory of suspension of soft particles, the colloid vibration current (CVI) model of suspended biological cells is investigated. A CVI measurement system is set up in which ultrasonic pulses of different intensities are calibrated and used. The experimental results on electrolyte solutions and cell suspensions show that CVI is closely related to the ion and cell concentration of the sample solution. Along with the increased concentration of ions or cells, the CVI signal increased initially and then decreased for electrolyte solutions, but it was the reverse for a chicken blood cell suspension but stayed almost constant for a spawn suspension. All these phenomena were caused by the interactive electrokinetic polarization of ions and cells under the action of ultrasonic pulses. Through the analysis of the electrokinetic course of the cell suspension, it was found that the amplitude and slope of the electrokinetic curve could be used to characterize the dielectric properties of cells. The electrokinetic model and CVI measurement system are rather feasible, as shown by the experimental results. The electrokinetic measurement method shows a new nondestructive way for studying the charging properties of biological cells, and the method has an inherent advantage for the real-time study of the biological effects of ultrasound on cells.

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Ultrasonic Characterization of Biological Cells

Cited by 7 publications

References 56 publications

Impact of extracorporeal shock waves on the human skin with cellulite: A case study of an unique instance

Impact of extracorporeal shock waves on the human skin with cellulite: A case study of an unique instance

High-Frequency Time-Resolved Scanning Acoustic Microscopy for Biomedical Applications

Study on the dielectric properties of biological cells using the electrokinetic method

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