Ultrasonic Extraction Process of Polysaccharides from Dendrobium nobile Lindl.: Optimization, Physicochemical Properties and Anti-Inflammatory Activity

Abstract

To optimize the ultrasonic extraction process of polysaccharides from Dendrobium nobile Lindl. (DNP), the extraction method was conducted through a single-factor test and the response-surface methodology (RSM). With the optimal extraction process (liquid–solid ratio of 40 mL/g, ultrasonic time of 30 min, and ultrasonic power of 400 W), the maximum extraction yield was 5.16 ± 0.41%. DNP1 and DNP2 were then fractionated via DEAE-QFF and Sephacryl S-300 HR chromatography. The molecular weight (Mw) of DNP1 was identified as 67.72 kDa, composed of Man (75.86 ± 0.05%) and Glc (24.14 ± 0.05%), and the Mw of DNP2 was 37.45 kDa, composed of Man (72.32 ± 0.03%) and Glc (27.68 ± 0.03%). Anti-inflammatory assays results showed that as DNPs were 200 μg/mL, and the contents of NO, TNF-α, IL-1β, IL-6 and IL-10 in LPS-induced RAW 264.7 cells were about 13.39% and 13.39%, 43.88% and 43.51%, 17.80% and 15.37%, 13.84% and 20.66%, and 938.85% and 907.77% of those in control group, respectively. It was indicated that DNP1 and DNP2 inhibited the inflammatory response of RAW 264.7 cells induced by LPS via suppressing the level of NO and pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) and promoting the secretion of anti-inflammatory cytokine (IL-10). Therefore, DNP1 and DNP2 have potential applications in the treatment of inflammatory injury.

Ultrasound-Assisted Extraction of Atractylodes chinensis (DC.) Koidz. Polysaccharides and the Synergistic Antigastric Cancer Effect in Combination with Oxaliplatin

“…The ultrasonication time is a key parameter affecting the yield of AKUs . The yield of AKUs increased rapidly as the extraction time increased and reached an extreme value of 12.40 ± 0.37% at 30 min (Figure B).…”

“…The ultrasonication time is a key parameter affecting the yield of AKUs. 28 The yield of AKUs increased rapidly as the extraction time increased and reached an extreme value of 12.40 ± 0.37% at 30 min (Figure 1B). When the extraction time was prolonged, the increase in thermal activity accelerated the outflow of polysaccharides from the raw materials, whereas the polysaccharides in the solution were degraded due to the time accumulation effect; thus, the yield of polysaccharides decreased.…”

Dendrobium nobile Lindl Polysaccharides Attenuate UVB‐induced Photodamage by Regulating Oxidative Stress, Inflammation and MMPs Expression in Mice Model

“…Polysaccharides extracted from plants are natural antioxidant components with unique advantages in skin care (24). Moreover, Dendrobium nobile Lindl polysaccharides (DNP) have been shown to have antioxidant (25), anti‐inflammatory (26), antiapoptotic (27,28) and immunomodulatory (29,30) functions in several disease models or in vitro experiments. Li (31) has recently reported protective effects of DNP against UVA‐induced photoaging in fibroblasts.…”

Photoprotective Effects of Dendrobium nobile Lindl. Polysaccharides against UVB-Induced Oxidative Stress and Apoptosis in HaCaT Cells

“…It is classified as a superior product in “Shen Nong’s Herbal Classic” because of its excellent tonic properties in alleviating paralysis, lowering Qi, and tonifying the weakness of the internal organs. Previous studies on DNL have mainly focused on its small molecule components, such as phenols, alkaloids, and sesquiterpenes [ 15 , 16 , 17 , 18 ], while only a few studies have investigated its bioactive macromolecules. DNL polysaccharides (DNPs) are among the main active components of DNL, exerting antioxidant [ 19 ], anti-inflammatory [ 17 ], and anti-apoptotic effects [ 20 , 21 ].…”

“…Previous studies on DNL have mainly focused on its small molecule components, such as phenols, alkaloids, and sesquiterpenes [ 15 , 16 , 17 , 18 ], while only a few studies have investigated its bioactive macromolecules. DNL polysaccharides (DNPs) are among the main active components of DNL, exerting antioxidant [ 19 ], anti-inflammatory [ 17 ], and anti-apoptotic effects [ 20 , 21 ]. Li [ 22 ] reported that DNPs ameliorate UVA-induced photoaging of fibroblasts.…”