Ultrasonic irradiation and SonoVue microbubbles-mediated RNA interference targeting PRR11 inhibits breast cancer cells proliferation and metastasis, but promotes apoptosis

Abstract

This study compared the effects of ultrasonic irradiation and SonoVue microbubbles (US) or Lipofectamine 3000 on the transfection of small interfering RNA for PRR11 (siPRR11) and PRR11 overexpression plasmid into breast cancer cells. SiPRR11 and PRR11 overexpression plasmid were transfected into breast cancer MCF7 cells mediated by US and Lipofectamine 3000. PRR11 expressions in breast cancer and normal tissues were determined using Gene Expression Profiling Interactive Analysis (GEPIA). The viability, proliferation, migration, invasion and apoptosis of breast cancer cells were respectively measured by MTT assay, clone formation assay, scratch wound-healing assay, Transwell assay and flow cytometry. PRR11 and epithelial-to-mesenchymal transition (EMT)-related and apoptosis-related (B-cell lymphoma 2, Bcl-2; Bcl-2 associated protein X, Bax) proteins expressions were by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot as appropriate. As ultrasonic intensity increased, the viability of MCF7 cells was decreased. Results from GEPIA suggested that PRR11 was up-regulated in breast cancer. Silencing PRR11 mediated by US showed a higher efficiency than by Lipofectamine 3000. SiPRR11 transfected by Lipofectamine 3000 suppressed cells growth and metastasis, while promoted cell apoptosis. Moreover, E-cadherin and Bax expressions were low but N-cadherin, Snail and Bcl-2 expressions were high. However, overexpressed PRR11 caused the opposite effects. More importantly, transfection of siPRR11 and PRR11 overexpression plasmid using US had a higher efficacy than using Lipofectamine 3000. US transfection of PRR11 siRNA showed better effects on inhibiting breast cancer progression. The current findings contribute to a novel treatment for breast cancer.

Ultrasound microbubble-mediated RNA interference targeting WNT1 inducible signaling pathway protein 1(WISP1) suppresses the proliferation and metastasis of breast cancer cells

“…Other studies highlighted that UTMD of miR-133a could inhibit the tumor growth and improve the survival rate in breast cancer mice [ 38 ], and UTMD of Cav1.3 siRNA exerted antitumor effects on breast cancer [ 39 ]. In addition to the aforementioned literature’s illustration of the feasibility of UTMD as a gene transfection modality to treat breast cancer, another study by Hui Luo et al evidenced that silenced PRR11 mediated by US exhibited a higher efficiency than that mediated by lipofectamine 3000 [ 40 ]. Besides, Xiaojiang Tang et al proved that the inhibitory effect of UTMD-mediated SOCS3 on the biological behavior of breast cancer cells was better than that of liposome-mediated SOCS3 [ 41 ].…”

PRR11 in Malignancies: Biological Activities and Targeted Therapies

“…Moreover, PRR11 blocked p85 homodimerization and sensitized to ligand-induced PI3K activation, suggesting that PRR11 amplification confers resistance to estrogen deprivation through hyperactivation of the PI3K pathway [ 1 ]. Notably, ultrasonic irradiation and SonoVue microbubbles-mediated RNA interference targeting PRR11 could exert anti-cancer effects via PRR11 [ 41 ]. However, the oncogenic role of PRR11 in vivo is still blurred, demanding additional exploration.…”

“…Preliminary experiments found that SonoVue microbubbles-mediated RNA interference targeting PRR11 could exert anti-cancer effects, forecasting the potential application of PRR11 for targeted therapy [ 9 , 41 ]. In the meanwhile, circRNA_cZNF292 was involved in angiogenesis of glioma through PRR11 [ 77 ].…”

“…Chalcone, modificated by dithiocarbamate scaffolds, exert inhibitory effects via PRR11 downregulation [ 30 ]. Furthermore, PRR11 was involved in the treatment of ultrasonic irradiation for breast cancer [ 41 ]. Therefore, targeting PRR11 might provide a reliable and promising therapy for human malignancies ( Figure 3 ).…”

Proline‐rich 11 (PRR11) promotes the progression of cutaneous squamous cell carcinoma by activating the EGFR signaling pathway

“…PRR11 is highly expressed in many human cancers and has been regarded as a potential biomarker for poor prognosis 9–14 . Moreover, numerous studies have shown that PRR11 participates in the tumorigenesis and development of many cancers, such as lung cancer, hepatocellular carcinoma, osteosarcoma, clear cell renal cell carcinoma, breast cancer, tongue squamous cell carcinoma (TSCC), esophageal squamous cell carcinoma, and pancreatic cancer 15–22 …”