Ultrasonographic and endocrine aspects of follicle deviation, and acquisition of ovulatory capacity in buffalo (Bubalus bubalis) heifers

Abstract

The objectives of this study were to determine the interval from ovulation to deviation and the diameter of the dominant (DF) and largest subordinate (SF) follicles at deviation in buffalo (Bubalus bubalis) heifers. Two methods of evaluation (observed vs. calculated) were used. FSH and LH profiles encompassing follicle deviation (Experiment 1), and the follicular diameter when the DF acquired ovulatory capacity (Experiment 2) were also determined. The time of deviation and the diameter of the DF and the largest SF at deviation did not differ between observed and calculated methods. Overall, follicle deviation occurred 2.6 ± 0.2d (mean ± SEM) after ovulation, and the diameters of the DF and SF at deviation were 7.2 ± 0.2 and 6.4 ± 0.2mm, respectively. No changes in plasma levels of FSH or LH were observed (P=0.32 and P=0.96, respectively). Experiment 2 was conducted in two phases according to the diameter of the DF during the first wave of follicular development at the time of LH challenge (25mg of pLH). In the first phase, follicles ranging from 5.0 to 6.0mm (n=7), 6.1 to 7.0mm (n=11), or 7.1 to 8.0mm (n=9) were used, and in the second phase, follicles ranging from 7.0 to 8.4mm (n=10), 8.5 to 10.0mm (n=10), or 10.1 to 12.0mm (n=9) of diameter were used. After the pLH treatment, the DF was monitored by ultrasonography every 12h for 48h. No ovulations occurred in heifers in the first phase. However, in the second phase, an effect of follicular diameter was observed on ovulation rate [7.0-8.4mm (0.0%, 0/10), 8.5-10.0mm (50.0%, 5/10), and 10.0-12.0mm (55.6%, 5/9)]. In summary, follicle deviation occurred 2.6d after ovulation in buffalo (B. bubalis) heifers, when the diameters of the DF and SF were 7.2 and 6.4mm, respectively. No significant changes in plasma concentrations of FSH or LH were detected. Finally, the acquisition of ovulatory capacity occurred when the DF reached 8.5mm in diameter.

Ultrasonographic Characterization of Follicle Deviation in Follicular Waves with Single Dominant and Codominant Follicles in Dromedary Camels (Camelus dromedarius)

“…) and closer to values reported in buffalo heifers (7.2 and 6.4 mm; Gimenes et al. ). These differences could be attributed to differences in growth rate of follicles between species.…”

“…) and buffalo heifers (Gimenes et al. ). The growth rate of F1 follicle between single and double ovulators was similar from the day of emergence to pre‐ovulatory stage (Palhao et al.…”

“…; Gimenes et al. ). Around the time of follicle deviation, a decrease in circulating FSH (Gastal et al.…”

Reference Gene Selection for Gene Expression Analysis of Oocytes Collected from Dairy Cattle and Buffaloes during Winter and Summer

“…A retrospective analysis was also conducted to choose the warmest and the coolest periods of the year in the farm where the experiment was carried out (data not shown). Murrah buffaloes ( B. bubalis ) used in the present work were maintained as described in a previous report [39]. As performed for cattle, two categories of buffaloes were used for oocyte collection: nulliparous (N) and multiparous (M).…”

First-Time Characterization of <i>JY-1</i>-Like Sequence in Goats

“…For example, embryo development is faster (12 -24 h) in buffaloes because of the early entry of embryos into the uterus (4 -5 days after oestrus) (Campanile et al, 2010). Another difference described by Gimenes et al (2011) is that during folliculogenesis in buffaloes there is no decrease in folliclestimulating hormone levels or increase in luteinizing hormone levels in blood plasma at the time of follicle recruitment. In addition, because protein JY-1 acts during the early stages of embryogenesis (when monozygotic embryos are formed) and in preovulatory events (when more than one oocyte may be recruited and ovulated at the same time, the origin of dizygotic twins), the protein difference may explain the difference in the percentage of multiple births among species.…”