ABSTRACT:Chronic cervical pain is a common source of disability in society, and evidence suggests that individuals with neck pain have impairment of the deep cervical fl exor (DCF) muscles. Th is study investigated the recruitment pattern of the neck muscles, particularly the DCF, during the Craniocervical Flexion Test (CCFT), using ultrasound measurement of muscle activity in asymptomatic subjects. In a cross-sectional design, 10 subjects, of both sexes, with no history of neck pain participated in the study. Participants were instructed to perform the CCFT, and changes in thickness from resting baseline values during the fi ve incremental stages of the test were obtained for DCF and sternocleidomastoid (SCM) muscles using ultrasonography. Th e most signifi cant changes found in DCF thickness were between phase 1 and phases 4 (p<0.001) and 5 (p= <0.001). For SCM, diff erences were most signifi cant between phases 1 and 3 (p<0.001), 4 (p<0.001), and 5 (p<0.001); and between phases 3 and 5 (p<0.003). No diff erences were found between DCF and SCM muscles. Th e present study confi rms the evidence that CCFT increases DCF recruitment. However, the ultrasonography test protocol did not identify diff erences in recruitment between deep and superfi cial neck muscles. Th e present study confi rms the evidence that the CCFT challenges the cervical spine and that DCF activity is increased during this maneuver.