“…The probe frequency had to be decreased to 4 MHz in claws with greater sole thickness in older buffaloes, which is consistent with the results of T Tsuka, R Nishimura, M Hishinuma, Y Murahata, M Yamashita, K Azuma, T Osaki, N Ito, Y Okamoto and T Imagawa [39], who reported that better images were obtained at lower ultrasound probe frequencies. Ultrasonography has been validated to provide objective measurements of the claws in cattle, and has been used to study, for example, the effect of changes in sole soft-tissue thickness and/or echogenicity on the development of claw horn disruption lesions [37,40,41], to objectively guide claw trimming [39,42], and to diagnose pedal bone fractures [43]. Here, we con rmed the utility of ultrasonography for examining the claws of buffaloes.…”