Ralstonia insidiosa (R. insidiosa) is a type of Ralstonia spp., which is a Gram-negative, non-fermenting bacterium. Ralstonia spp. have recently been regarded as emerging pathogens of infectious diseases, but R. insidiosa infections have rarely been reported. 1 Here, we report a case of an acute focal bacterial nephritis (AFBN) caused by R. insidiosa in a 9-year-old boy.A healthy 9-year-old Japanese boy presented to our hospital with a 2-day history of fever, vomiting, and diarrhea. He had no history of urinary tract infection (UTI) and had been vaccinated as scheduled. Physical examination revealed abdominal and bilateral costovertebral angle tenderness. Laboratory parameters were as follows: white blood cell count, 19,000/ μL; neutrophils, 89.3%; serum C-reactive protein level, 16.15 mg/dL; blood urea nitrogen level, 11.5 mg/dL; creatinine, 0.55 mg/dL (estimated Glomerular Filtration Rate, 82.47 mL/min/1.73 m 2 ); immunoglobulin G (IgG), 1608 mg/dL; immunoglobulin A (IgA), 266 mg/dL; immunoglobulin M (IgM), 227 mg/dL; C3, 186 mg/dL; C4, 30.4 mg/dL; CH50, 48 U/mL; measles IgG, 20.5(+); and measles IgM, 0.06(−). Urinalysis showed no pyuria or nitrite urine, but Gram staining of the urine showed Gram-negative rods. Chest radiograph was normal. Renal ultrasound showed an enlarged right kidney with a hyperechoic mass and reduced blood flow at the upper pole, which was suspected to be AFBN. Contrastenhanced computed tomography (CT) demonstrated bilateral lesions with heterogeneously decreased nephrographic density and a dilated left ureter (Figure 1a-c). The right kidney was enlarged to 95 mm (mean + 2.0 standard deviation), and the left kidney was atrophied to 70 mm (mean − 2.5 standard deviation). 2 We therefore diagnosed the patient with AFBN with congenital anomalies of the kidney and urinary tract (CAKUT). Intravenous ampicillin/sulbactam (150 mg/kg/day) was then administered. The patient's fever persisted, so the