Ultrasound accelerated gelation of novel l-lysine based hydrogelators

Shangfeng, Pan; Luo, Song; Li, Sai; Lai, Yusi; Geng, Yanyan; He, Bin; Gu, Zhongwei

doi:10.1039/c3cc44767g

Cited by 38 publications

(27 citation statements)

References 31 publications

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“…3(b)). It implied that the red shift was due to the interaction between MTX and polyrotaxane nanoparticles [20,21]. The FT-IR spectra of MTX, polyrotaxane nanoparticles and MTX loaded polyrotaxane nanoparticles were presented in Fig.…”

Section: Resultsmentioning

confidence: 97%

Self-assembly Polyrotaxanes Nanoparticles as Carriers for Anticancer Drug Methotrexate Delivery

Zhang

et al. 2014

Nano-Micro Lett.

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α-Cyclodextrin/poly(ethylene glycol) (α-CD/PEG) polyrotaxane nanoparticles were prepared via a self-assembly method. Anticancer drug methotrexate (MTX) was loaded in the nanoparticles. The interaction between MTX and polyrotaxane was investigated. The formation, morphology, drug release and in vitro anticancer activity of the MTX loaded polyrotaxane nanoparticles were studied. The results show that the MTX could be efficiently absorbed on the nanoparticles, and hydrogen bonds were formed between MTX and α-CDs. The typical channel-type stacking assembly style of polyrotaxane nanoparticles was changed after MTX was loaded. The mean diameter of drug loaded polyrotaxane nanoparticles were around 200 nm and the drug loading content was as high as about 20%. Drug release profiles show that most of the loaded MTX was released within 8 hours and the cumulated release rate was as high as 98%. The blank polyrotaxane nanoparticles were nontoxicity to cells. The in vitro anticancer activity of the MTX loaded polyrotaxane nanoparticles was higher than that of free MTX.

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Section: Resultsmentioning

confidence: 97%

Self-assembly Polyrotaxanes Nanoparticles as Carriers for Anticancer Drug Methotrexate Delivery

Zhang

et al. 2014

Nano-Micro Lett.

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“…Hydrogels are three-dimensional polymeric networks made of highly hydrophilic monomers (Hoare and Kohane, 2008;Pan et al, 2013;Wei et al, 2016;Glass et al, 2018). In the past few decades, numerous hydrogels have been developed based on natural and synthetic molecules such as cellulose, chitosan, polypeptide and poly(acrylic acid), poly(ethylene glycol), and poly(ethylene glycol) diacrylate (PEGDA).…”

Section: Introductionmentioning

confidence: 99%

A PEGDA/DNA Hybrid Hydrogel for Cell-Free Protein Synthesis

Cui

Sun

et al. 2020

Front. Chem.

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Cell-free protein synthesis (CFPS) has the advantage of rapid expression of proteins and has been widely implemented in synthetic biology and protein engineering. However, the critical problem limiting CFPS industrial application is its relatively high cost, which partly attributes to the overexpense of single-use DNA templates. Hydrogels provide a possible solution because they can preserve and reutilize the DNA templates in CFPS and have great potential in elevating the protein production yield of the CFPS. Here, we presented a low-cost hybrid hydrogel simply prepared with polyethylene glycol diacrylate (PEGDA) and DNA, which is capable of high-efficient and repeated protein synthesis in CFPS. Parameters governing protein production specific to hybrid hydrogels were optimized. Structures and physical properties of the hybrid hydrogel were characterized. Transcription and expression kinetics of solution phase system and gel phased systems were investigated. The results showed that PEGDA/DNA hydrogel can enhance the protein expression of the CFPS system and enable a repeated protein production for tens of times. This PEGDA/DNA hybrid hydrogel can serve as a recyclable gene carrier for either batch or continuous protein expression, and paves a path toward more powerful, scalable protein production and cell-free synthetic biology.

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“…Recently, ultrasound is successfully utilized to induce the gelation of organic liquids to form new products and novel architectures [13,14]. Sonication also can induce aggregation of protein similar to gelation [15].…”

Section: Introductionmentioning

confidence: 99%