Ultrasound‐assisted aqueous two‐phase extraction of phenylethanoid glycosides from <i>Cistanche deserticola</i> Y. C. Ma stems

Dong, Bo; Yuan, Xiaofan; Zhao, Qingsheng; Feng, Qingming; Liu, Boyan; Guo, Yongbo; Zhao, Bing

doi:10.1002/jssc.201401410

Cited by 37 publications

(30 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, high temperature can cause inactivation or degradation of active compounds. Thus, the extraction should be performed at mild temperature . To investigate the effect of temperature on the SOE of sinomenine, the temperature range 20–60 ° C was chosen.…”

Section: Resultsmentioning

confidence: 99%

Salting‐out extraction of sinomenine from Sinomenium acutum by an alcohol/salt aqueous two‐phase system using ionic liquids as additives

Qiao

et al. 2017

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: The aqueous two-phase extraction (ATPE) based on organic solvent and inorganic salt was considered a promising method in the extraction, enrichment, and purification field. Ionic liquids (ILs) used as additives to the aqueous two-phase system (ATPS) can improve the extraction yield. RESULTS: In this study, sevenILs were added to the ethanol/ammonium sulfate ATPS and used for the salting-out extraction (SOE) of sinomenine from Sinomenium acutum. The IL [C 2 OHmim]FeCl 4 was screened as the optimal additive, then the influencing factors IL amount, ethanol concentration, salt concentration, and temperature were investigated. The maximum extraction efficiency of 96.4% was obtained under the following conditions: 23.06 wt% ethanol concentration, 27.28 wt% (NH 4 ) 2 SO 4 concentration, 4.0 wt% [C 2 OHmim]FeCl 4 and 40 ∘ C temperature. Moreover, the components in the SOE system can be recycled after extraction. CONCLUSION: Thus, this ATPS had lower cost and higher extraction ability, and it is feasible to scale up using a small amount of ILs. This system can also be used in the extraction of some other important active compounds from natural plant resources.

show abstract

Section: Resultsmentioning

confidence: 99%

Salting‐out extraction of sinomenine from Sinomenium acutum by an alcohol/salt aqueous two‐phase system using ionic liquids as additives

Qiao

et al. 2017

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…The synergistic effect of UAATPS through ultrasonic cell wall disintegration and diffusion of the solvent into the cell has a positive impact on the polysaccharide extract thus, significantly increasing mass transfers of the active nutrients (Chemat & Khan, 2011;Ji, Peng, Yuan, Liu, & Wang, 2018). However, this technique has been associated with initiating the degradation of polysaccharides due to prolonged ultrasonic treatment (Dong et al, 2015). In recent times, alternative modes to mono frequency irradiation such as dual-frequency (DFu) have emerged and reported to have a better cavitation and product yield through a simultaneous effect of low and high frequencies (Muatasim, Ma, & Yang, 2018).…”

Section: Introductionmentioning

confidence: 99%

Dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase extraction and purification of Astragalus polysaccharides

Chikari

Han

Wang

et al. 2020

J Food Process Engineering

View full text Add to dashboard Cite

The study investigated the effect of dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase extraction and desalination on the yield, microstructure and antioxidant properties of Astragalus polysaccharides (APS). Extracts were desalinated for 10 (APS10), 20 (APS20), and 30 (APS30) minutes. A 28.40% experimental yield, well correlated to the response surface methodology predicted model was achieved. Thermodynamic parameters were non‐spontaneous, irreversible and endothermic. Moreover, ultrasound enhanced the desalination rate and did not alter the chemical structure in the APS. Fourier Transform Infrared Spectroscopy showed a similar spectrum in all the APS samples. High‐performance gel chromatography and scanning electron microscopy, respectively presented reduced molecular weights and altered microstructures in the samples. Monosaccharide analysis revealed the existence of xylose, mannose, galactose, glucose, arabinose, rhamnose, and ribose in the APS samples. Moreover, the APS20 showed strong hydroxyl and 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging activities, thus showing its potency in reducing oxidative stress. Hence, this study demonstrates that coupled ultrasound and alcohol/salt ATPS is a sustainable technique, which generates high yields of simultaneously extracted products. Practical applications Astragalus root has been used as an effective complementary treatment to conventional medicine owing to its rich nutraceutical composition and its numerous health attributes. Commercialization of Astragalus supplements into functional foods and nutraceuticals requires continuous exploration of novel, low cost and reproducible methods that retain their bioactivity. In this work, a synergized and bio‐compatible dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase system was used to extract and purify Astragalus polysaccharides. Application of this technique in the nutraceutical industry reduces treatment time, lowers bulk energy consumption hence reducing energy costs. It promotes the use of low‐cost, non‐hazardous bio‐based solvents in treatment processes thus considerably reducing extraction costs and environmental degradation. Findings from this study exhibit the practicability of this technique in industrial application. It equips biotechnologists with information on improved, sustainable and safer ways of extracting and enriching the quality of nutraceutical compounds.

show abstract

“…At present, the research of C. tubulosa mainly focuses on its pharmacological components. The separation and purification methods mainly include macroporous adsorption resin, highspeed countercurrent chromatography, membrane separation, and molecular imprinting (Han et al, 2012;Dong et al, 2015;Zhang et al, 2018a;Pei et al, 2019;Si et al, 2019). Macroporous adsorption resin is a mature technology, but there are some shortcomings such as a large amount of solvent, long time, low yield, and complex process.…”

Section: Introductionmentioning

confidence: 99%

Selective Adsorption and Purification of the Acteoside in Cistanche tubulosa by Molecularly Imprinted Polymers

2020

View full text Add to dashboard Cite

Acteoside (ACT) is the main component of phenylethanoid glycosides in Cistanche tubulosa, and it is extremely desirable for obtaining high purification of ACT by molecularly imprinted polymers (MIPs) from their extracts. In this study, MIPs were designed and synthetized to adsorb selectively the ACT in C. tubulosa. The effects of different functional monomers, cross-linkers, and solvents of MIPs were investigated. MIPs were studied in terms of static adsorption experiments, dynamic adsorption experiments, and selectivity experiments. The optimal functional monomer, cross-linking agent, and solvent are 4-vinylpyridine, ethylene glycol dimethylacrylate, and the mixed solvent (acetonitrile and N,N-dimethylformamide, 1:1.5, v/v), respectively. Under the optimal conditions, the synthesized MIP1 has a high adsorption performance for ACT. The adsorption capacity of MIP1 to ACT reached 112.60 mg/g, and the separation factor of ACT/echinacoside was 4.68. Because the molecularly imprinted cavities of MIP1 resulted from template molecules of ACT, it enables MIP1 to recognize selectively ACT. Moreover, the N-H groups on MIP1 can form hydrogen bonds with the hydroxyl groups on the ACT; this improves the separation factor of MIP1. The dynamic adsorption of ACT accorded with the quasi-second-order kinetics; it indicated that the adsorption process of MIP1 is the process of chemical adsorption to ACT. MIPs can be applied as a potential adsorption material to purify the active ingredients of herbal medicines.

show abstract

Ultrasound‐assisted aqueous two‐phase extraction of phenylethanoid glycosides from Cistanche deserticola Y. C. Ma stems

Cited by 37 publications

References 22 publications

Salting‐out extraction of sinomenine from Sinomenium acutum by an alcohol/salt aqueous two‐phase system using ionic liquids as additives

Salting‐out extraction of sinomenine from Sinomenium acutum by an alcohol/salt aqueous two‐phase system using ionic liquids as additives

Dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase extraction and purification of Astragalus polysaccharides

Selective Adsorption and Purification of the Acteoside in Cistanche tubulosa by Molecularly Imprinted Polymers

Contact Info

Product

Resources

About